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Characterizing the role of rice NRAMP5 in Manganese, Iron and Cadmium Transport.

Ishimaru Y, Takahashi R, Bashir K, Shimo H, Senoura T, Sugimoto K, Ono K, Yano M, Ishikawa S, Arao T, Nakanishi H, Nishizawa NK - Sci Rep (2012)

Bottom Line: OsNRAMP5 expression was restricted to roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem.The suppression of OsNRAMP5 promoted Cd translocation to shoots, highlighting the importance of this gene for Cd phytoremediation.These data reveal that OsNRAMP5 contributes to Mn, Cd, and Fe transport in rice and is important for plant growth and development.

View Article: PubMed Central - PubMed

ABSTRACT
Metals like manganese (Mn) and iron (Fe) are essential for metabolism, while cadmium (Cd) is toxic for virtually all living organisms. Understanding the transport of these metals is important for breeding better crops. We have identified that OsNRAMP5 contributes to Mn, Fe and Cd transport in rice. OsNRAMP5 expression was restricted to roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem. OsNRAMP5 localized to the plasma membrane, and complemented the growth of yeast strains defective in Mn, Fe, and Cd transport. OsNRAMP5 RNAi (OsNRAMP5i) plants accumulated less Mn in the roots, and less Mn and Fe in shoots, and xylem sap. The suppression of OsNRAMP5 promoted Cd translocation to shoots, highlighting the importance of this gene for Cd phytoremediation. These data reveal that OsNRAMP5 contributes to Mn, Cd, and Fe transport in rice and is important for plant growth and development.

No MeSH data available.


Related in: MedlinePlus

OsNRAMP5i Anjana Dhan for Cd phytoremediation.(a) Phenotypes of four-week-old wild type and OsNRAMP5i (A5i-2) Anjana Dhan plants transferred to a nutrient solution containing 10 µM CdCl2 and cultivated for two weeks. Scale bars = 10 cm. (b) OsNRAMP5 transcripts in the roots of wild type and OsNRAMP5i (A5i-1, A5i-2, A5i-3, A5i-4) Anjana Dhan plants grown in normal nutrient hydroponic culture. Shoot Mn (c), Fe (d) concentration, shoot dry weight (e), shoot Cd concentration (f) and Shoot Cd content (g) of wild type and A5i plants grown under 10 μM Cd condition. Error bars represent the SD. Columns bars followed by different letters are statistically different according to analysis of variance followed by SNK test (b, p0.0000; e, p = 0.0113; f, p = 0.0000; g, p = 0.0000); n = 3.
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f7: OsNRAMP5i Anjana Dhan for Cd phytoremediation.(a) Phenotypes of four-week-old wild type and OsNRAMP5i (A5i-2) Anjana Dhan plants transferred to a nutrient solution containing 10 µM CdCl2 and cultivated for two weeks. Scale bars = 10 cm. (b) OsNRAMP5 transcripts in the roots of wild type and OsNRAMP5i (A5i-1, A5i-2, A5i-3, A5i-4) Anjana Dhan plants grown in normal nutrient hydroponic culture. Shoot Mn (c), Fe (d) concentration, shoot dry weight (e), shoot Cd concentration (f) and Shoot Cd content (g) of wild type and A5i plants grown under 10 μM Cd condition. Error bars represent the SD. Columns bars followed by different letters are statistically different according to analysis of variance followed by SNK test (b, p0.0000; e, p = 0.0113; f, p = 0.0000; g, p = 0.0000); n = 3.

Mentions: As the OsNRAMP5i (T5i) shoots accumulated more Cd than did wild type shoots, we developed OsNRAMP5i plants (A5i) using a high Cd accumulating cultivar, Anjana Dhan38. There was no significant difference in shoot length between the Anjana Dhan OsNRAMP5i (A5i) and wild type plants when plants were grown in the presence of 10 µM Cd (Fig. 7a). OsNRAMP5 expression in the A5i roots was 33–66% less than that in wild type when grown under normal nutrient conditions (Fig. 7b). At 10 µM Cd conditions, slightly less Mn accumulated in the A5i shoots and there was no difference in Fe concentration (Fig. 7c and d). Moreover, there was no significant difference in shoot weight; however, the shoot Cd concentration in A5i was up to 4.9 times that in wild type (Fig. 7e and f) and the shoot Cd content in A5i was up to 4.3 times greater than that in wild type (Fig. 7g).


Characterizing the role of rice NRAMP5 in Manganese, Iron and Cadmium Transport.

Ishimaru Y, Takahashi R, Bashir K, Shimo H, Senoura T, Sugimoto K, Ono K, Yano M, Ishikawa S, Arao T, Nakanishi H, Nishizawa NK - Sci Rep (2012)

OsNRAMP5i Anjana Dhan for Cd phytoremediation.(a) Phenotypes of four-week-old wild type and OsNRAMP5i (A5i-2) Anjana Dhan plants transferred to a nutrient solution containing 10 µM CdCl2 and cultivated for two weeks. Scale bars = 10 cm. (b) OsNRAMP5 transcripts in the roots of wild type and OsNRAMP5i (A5i-1, A5i-2, A5i-3, A5i-4) Anjana Dhan plants grown in normal nutrient hydroponic culture. Shoot Mn (c), Fe (d) concentration, shoot dry weight (e), shoot Cd concentration (f) and Shoot Cd content (g) of wild type and A5i plants grown under 10 μM Cd condition. Error bars represent the SD. Columns bars followed by different letters are statistically different according to analysis of variance followed by SNK test (b, p0.0000; e, p = 0.0113; f, p = 0.0000; g, p = 0.0000); n = 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3285952&req=5

f7: OsNRAMP5i Anjana Dhan for Cd phytoremediation.(a) Phenotypes of four-week-old wild type and OsNRAMP5i (A5i-2) Anjana Dhan plants transferred to a nutrient solution containing 10 µM CdCl2 and cultivated for two weeks. Scale bars = 10 cm. (b) OsNRAMP5 transcripts in the roots of wild type and OsNRAMP5i (A5i-1, A5i-2, A5i-3, A5i-4) Anjana Dhan plants grown in normal nutrient hydroponic culture. Shoot Mn (c), Fe (d) concentration, shoot dry weight (e), shoot Cd concentration (f) and Shoot Cd content (g) of wild type and A5i plants grown under 10 μM Cd condition. Error bars represent the SD. Columns bars followed by different letters are statistically different according to analysis of variance followed by SNK test (b, p0.0000; e, p = 0.0113; f, p = 0.0000; g, p = 0.0000); n = 3.
Mentions: As the OsNRAMP5i (T5i) shoots accumulated more Cd than did wild type shoots, we developed OsNRAMP5i plants (A5i) using a high Cd accumulating cultivar, Anjana Dhan38. There was no significant difference in shoot length between the Anjana Dhan OsNRAMP5i (A5i) and wild type plants when plants were grown in the presence of 10 µM Cd (Fig. 7a). OsNRAMP5 expression in the A5i roots was 33–66% less than that in wild type when grown under normal nutrient conditions (Fig. 7b). At 10 µM Cd conditions, slightly less Mn accumulated in the A5i shoots and there was no difference in Fe concentration (Fig. 7c and d). Moreover, there was no significant difference in shoot weight; however, the shoot Cd concentration in A5i was up to 4.9 times that in wild type (Fig. 7e and f) and the shoot Cd content in A5i was up to 4.3 times greater than that in wild type (Fig. 7g).

Bottom Line: OsNRAMP5 expression was restricted to roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem.The suppression of OsNRAMP5 promoted Cd translocation to shoots, highlighting the importance of this gene for Cd phytoremediation.These data reveal that OsNRAMP5 contributes to Mn, Cd, and Fe transport in rice and is important for plant growth and development.

View Article: PubMed Central - PubMed

ABSTRACT
Metals like manganese (Mn) and iron (Fe) are essential for metabolism, while cadmium (Cd) is toxic for virtually all living organisms. Understanding the transport of these metals is important for breeding better crops. We have identified that OsNRAMP5 contributes to Mn, Fe and Cd transport in rice. OsNRAMP5 expression was restricted to roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem. OsNRAMP5 localized to the plasma membrane, and complemented the growth of yeast strains defective in Mn, Fe, and Cd transport. OsNRAMP5 RNAi (OsNRAMP5i) plants accumulated less Mn in the roots, and less Mn and Fe in shoots, and xylem sap. The suppression of OsNRAMP5 promoted Cd translocation to shoots, highlighting the importance of this gene for Cd phytoremediation. These data reveal that OsNRAMP5 contributes to Mn, Cd, and Fe transport in rice and is important for plant growth and development.

No MeSH data available.


Related in: MedlinePlus