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Characterizing the role of rice NRAMP5 in Manganese, Iron and Cadmium Transport.

Ishimaru Y, Takahashi R, Bashir K, Shimo H, Senoura T, Sugimoto K, Ono K, Yano M, Ishikawa S, Arao T, Nakanishi H, Nishizawa NK - Sci Rep (2012)

Bottom Line: OsNRAMP5 expression was restricted to roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem.The suppression of OsNRAMP5 promoted Cd translocation to shoots, highlighting the importance of this gene for Cd phytoremediation.These data reveal that OsNRAMP5 contributes to Mn, Cd, and Fe transport in rice and is important for plant growth and development.

View Article: PubMed Central - PubMed

ABSTRACT
Metals like manganese (Mn) and iron (Fe) are essential for metabolism, while cadmium (Cd) is toxic for virtually all living organisms. Understanding the transport of these metals is important for breeding better crops. We have identified that OsNRAMP5 contributes to Mn, Fe and Cd transport in rice. OsNRAMP5 expression was restricted to roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem. OsNRAMP5 localized to the plasma membrane, and complemented the growth of yeast strains defective in Mn, Fe, and Cd transport. OsNRAMP5 RNAi (OsNRAMP5i) plants accumulated less Mn in the roots, and less Mn and Fe in shoots, and xylem sap. The suppression of OsNRAMP5 promoted Cd translocation to shoots, highlighting the importance of this gene for Cd phytoremediation. These data reveal that OsNRAMP5 contributes to Mn, Cd, and Fe transport in rice and is important for plant growth and development.

No MeSH data available.


Related in: MedlinePlus

Xylem sap metal concentrations in OsNRAMP5i plants.Xylem Mn (a), Fe (b), and Ca (c) concentrations in T5i plants grown under control or Mn-deficient (-Mn) conditions. Error bars represent the SD. Columns bars followed by different letters are statistically different according to analysis of variance followed by SNK test (a, p = 0.0001 (Ctrl.) & p = 0.0010 (-Mn); b, p = 0.0000 (Ctrl.) & p = 0.0001 (-Mn); n = 3.
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f5: Xylem sap metal concentrations in OsNRAMP5i plants.Xylem Mn (a), Fe (b), and Ca (c) concentrations in T5i plants grown under control or Mn-deficient (-Mn) conditions. Error bars represent the SD. Columns bars followed by different letters are statistically different according to analysis of variance followed by SNK test (a, p = 0.0001 (Ctrl.) & p = 0.0010 (-Mn); b, p = 0.0000 (Ctrl.) & p = 0.0001 (-Mn); n = 3.

Mentions: In plants grown under Mn-deficient conditions, the root and shoot length and SPAD value of the T5i lines were significantly decreased (Fig. 4d–g). The growth of the OX lines was also affected in terms of shoot length and SPAD value (Fig. 4f and g). The T5i lines accumulated less Mn than wild type plants both in roots and shoots (Fig. 4h and i), while the Mn concentration in the OX plants was similar to that in wild type roots (Fig. 4h). The Mn and Fe concentrations in xylem sap from the T5i lines were also significantly reduced compared to those in wild type plants (Fig. 5a and b), whereas no difference was observed in the Ca concentration (Fig. 5c). Under Fe-deficient conditions, there were no significant differences in plant growth, SPAD value, and metal concentrations (Supplementary Fig. 4).


Characterizing the role of rice NRAMP5 in Manganese, Iron and Cadmium Transport.

Ishimaru Y, Takahashi R, Bashir K, Shimo H, Senoura T, Sugimoto K, Ono K, Yano M, Ishikawa S, Arao T, Nakanishi H, Nishizawa NK - Sci Rep (2012)

Xylem sap metal concentrations in OsNRAMP5i plants.Xylem Mn (a), Fe (b), and Ca (c) concentrations in T5i plants grown under control or Mn-deficient (-Mn) conditions. Error bars represent the SD. Columns bars followed by different letters are statistically different according to analysis of variance followed by SNK test (a, p = 0.0001 (Ctrl.) & p = 0.0010 (-Mn); b, p = 0.0000 (Ctrl.) & p = 0.0001 (-Mn); n = 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3285952&req=5

f5: Xylem sap metal concentrations in OsNRAMP5i plants.Xylem Mn (a), Fe (b), and Ca (c) concentrations in T5i plants grown under control or Mn-deficient (-Mn) conditions. Error bars represent the SD. Columns bars followed by different letters are statistically different according to analysis of variance followed by SNK test (a, p = 0.0001 (Ctrl.) & p = 0.0010 (-Mn); b, p = 0.0000 (Ctrl.) & p = 0.0001 (-Mn); n = 3.
Mentions: In plants grown under Mn-deficient conditions, the root and shoot length and SPAD value of the T5i lines were significantly decreased (Fig. 4d–g). The growth of the OX lines was also affected in terms of shoot length and SPAD value (Fig. 4f and g). The T5i lines accumulated less Mn than wild type plants both in roots and shoots (Fig. 4h and i), while the Mn concentration in the OX plants was similar to that in wild type roots (Fig. 4h). The Mn and Fe concentrations in xylem sap from the T5i lines were also significantly reduced compared to those in wild type plants (Fig. 5a and b), whereas no difference was observed in the Ca concentration (Fig. 5c). Under Fe-deficient conditions, there were no significant differences in plant growth, SPAD value, and metal concentrations (Supplementary Fig. 4).

Bottom Line: OsNRAMP5 expression was restricted to roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem.The suppression of OsNRAMP5 promoted Cd translocation to shoots, highlighting the importance of this gene for Cd phytoremediation.These data reveal that OsNRAMP5 contributes to Mn, Cd, and Fe transport in rice and is important for plant growth and development.

View Article: PubMed Central - PubMed

ABSTRACT
Metals like manganese (Mn) and iron (Fe) are essential for metabolism, while cadmium (Cd) is toxic for virtually all living organisms. Understanding the transport of these metals is important for breeding better crops. We have identified that OsNRAMP5 contributes to Mn, Fe and Cd transport in rice. OsNRAMP5 expression was restricted to roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem. OsNRAMP5 localized to the plasma membrane, and complemented the growth of yeast strains defective in Mn, Fe, and Cd transport. OsNRAMP5 RNAi (OsNRAMP5i) plants accumulated less Mn in the roots, and less Mn and Fe in shoots, and xylem sap. The suppression of OsNRAMP5 promoted Cd translocation to shoots, highlighting the importance of this gene for Cd phytoremediation. These data reveal that OsNRAMP5 contributes to Mn, Cd, and Fe transport in rice and is important for plant growth and development.

No MeSH data available.


Related in: MedlinePlus