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Cardiac and Metabolic Physiology of Early Larval Zebrafish (Danio rerio) Reflects Parental Swimming Stamina.

Gore M, Burggren WW - Front Physiol (2012)

Bottom Line: However, significant (P < 0.05) differences existed between the L(LSD) and L(HSD) populations at 5 and 14 dpf in f(H),r and at days 10 and 15 dpf in f(H),a. [Formula: see text] was 0.04-0.32 μmol mg(-1) h(-1), while [Formula: see text] was 0.2-1.2 μmol mg(-1) h(-1).Gross cost of transport was ∼6-10 μmol O(2)·μg(-1) m(-1) at 5 dpf, peaking at 14-19 μmol O(2) μg(-1) m(-1) at 7-10 dpf, before falling again to 5-6 μmol O(2) μg(-1) m(-1) at 21 dpf, with gross cost of transport significantly higher in the L(LSD) population at 7 dpf.Collectively, these data indicate that inherited physiological differences known to contribute to enhanced stamina in adult parents also appear in their larval offspring well before attainment of juvenile or adult features.

View Article: PubMed Central - PubMed

Affiliation: Developmental Integrative Biology Cluster, Department of Biological Sciences, University of North Texas Denton, TX, USA.

ABSTRACT
Swimming stamina in adult fish is heritable, it is unknown if inherited traits that support enhanced swimming stamina in offspring appear only in juveniles and/or adults, or if these traits actually appear earlier in the morphologically quite different larvae. To answer this question, mature adult zebrafish (Danio rerio) were subjected to a swimming performance test that allowed separation into low swimming stamina or high swimming stamina groups. Adults were then bred within their own performance groups. Larval offspring from each of the two groups, designated high (L(HSD)) and low stamina-derived larvae (L(LSD)), were then reared at 27°C in aerated water (21% O(2)). Routine (f(H),r) and active (f(H),a) heart rate, and routine [Formula: see text] and active [Formula: see text] mass-specific oxygen consumption were recorded from 5 days post fertilization (dpf) through 21 dpf, and gross cost of transport and factorial aerobic metabolic scope were derived from [Formula: see text] measurements. Heart rate generally ranged between 150 and 225 bpm in both L(HSD) and L(LSD) populations. However, significant (P < 0.05) differences existed between the L(LSD) and L(HSD) populations at 5 and 14 dpf in f(H),r and at days 10 and 15 dpf in f(H),a. [Formula: see text] was 0.04-0.32 μmol mg(-1) h(-1), while [Formula: see text] was 0.2-1.2 μmol mg(-1) h(-1). Significant (P < 0.05) differences between the L(LSD) and L(HSD) populations in [Formula: see text] occurred at 7, 10, and 21 dpf and in [Formula: see text] at 7 dpf. Gross cost of transport was ∼6-10 μmol O(2)·μg(-1) m(-1) at 5 dpf, peaking at 14-19 μmol O(2) μg(-1) m(-1) at 7-10 dpf, before falling again to 5-6 μmol O(2) μg(-1) m(-1) at 21 dpf, with gross cost of transport significantly higher in the L(LSD) population at 7 dpf. Collectively, these data indicate that inherited physiological differences known to contribute to enhanced stamina in adult parents also appear in their larval offspring well before attainment of juvenile or adult features.

No MeSH data available.


Related in: MedlinePlus

Routine (fH,r) and active (fH,a) heart rate of high stamina-derived larvae (LHSD) and low stamina-derived larvae (LLSD) as a function of development. Values plotted are means ± 1 SE; n values in parentheses. Boxes enclose statistically identical values.
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Figure 2: Routine (fH,r) and active (fH,a) heart rate of high stamina-derived larvae (LHSD) and low stamina-derived larvae (LLSD) as a function of development. Values plotted are means ± 1 SE; n values in parentheses. Boxes enclose statistically identical values.

Mentions: Heart rate differed significantly between larvae from low and high stamina parents (two-way ANOVA on ranks, P < 0.05; Figure 2). LLSD had significantly higher routine heart rates at 7, and 14 and 21 dpf (P < 0.001). fH,a recorded from LHSD and LLSD (Figure 2) were also significantly different (two-way ANOVA on ranks, P < 0.05), with the LLSD showing significantly higher fH,a at 10 and 14 dpf (P < 0.05 and P < 0.001). There were also significant differences between fH,r and fH,a within LHSD (P < 0.001) and LLSD (P < 0.001), with a higher fH,a for all developmental stages.


Cardiac and Metabolic Physiology of Early Larval Zebrafish (Danio rerio) Reflects Parental Swimming Stamina.

Gore M, Burggren WW - Front Physiol (2012)

Routine (fH,r) and active (fH,a) heart rate of high stamina-derived larvae (LHSD) and low stamina-derived larvae (LLSD) as a function of development. Values plotted are means ± 1 SE; n values in parentheses. Boxes enclose statistically identical values.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3285806&req=5

Figure 2: Routine (fH,r) and active (fH,a) heart rate of high stamina-derived larvae (LHSD) and low stamina-derived larvae (LLSD) as a function of development. Values plotted are means ± 1 SE; n values in parentheses. Boxes enclose statistically identical values.
Mentions: Heart rate differed significantly between larvae from low and high stamina parents (two-way ANOVA on ranks, P < 0.05; Figure 2). LLSD had significantly higher routine heart rates at 7, and 14 and 21 dpf (P < 0.001). fH,a recorded from LHSD and LLSD (Figure 2) were also significantly different (two-way ANOVA on ranks, P < 0.05), with the LLSD showing significantly higher fH,a at 10 and 14 dpf (P < 0.05 and P < 0.001). There were also significant differences between fH,r and fH,a within LHSD (P < 0.001) and LLSD (P < 0.001), with a higher fH,a for all developmental stages.

Bottom Line: However, significant (P < 0.05) differences existed between the L(LSD) and L(HSD) populations at 5 and 14 dpf in f(H),r and at days 10 and 15 dpf in f(H),a. [Formula: see text] was 0.04-0.32 μmol mg(-1) h(-1), while [Formula: see text] was 0.2-1.2 μmol mg(-1) h(-1).Gross cost of transport was ∼6-10 μmol O(2)·μg(-1) m(-1) at 5 dpf, peaking at 14-19 μmol O(2) μg(-1) m(-1) at 7-10 dpf, before falling again to 5-6 μmol O(2) μg(-1) m(-1) at 21 dpf, with gross cost of transport significantly higher in the L(LSD) population at 7 dpf.Collectively, these data indicate that inherited physiological differences known to contribute to enhanced stamina in adult parents also appear in their larval offspring well before attainment of juvenile or adult features.

View Article: PubMed Central - PubMed

Affiliation: Developmental Integrative Biology Cluster, Department of Biological Sciences, University of North Texas Denton, TX, USA.

ABSTRACT
Swimming stamina in adult fish is heritable, it is unknown if inherited traits that support enhanced swimming stamina in offspring appear only in juveniles and/or adults, or if these traits actually appear earlier in the morphologically quite different larvae. To answer this question, mature adult zebrafish (Danio rerio) were subjected to a swimming performance test that allowed separation into low swimming stamina or high swimming stamina groups. Adults were then bred within their own performance groups. Larval offspring from each of the two groups, designated high (L(HSD)) and low stamina-derived larvae (L(LSD)), were then reared at 27°C in aerated water (21% O(2)). Routine (f(H),r) and active (f(H),a) heart rate, and routine [Formula: see text] and active [Formula: see text] mass-specific oxygen consumption were recorded from 5 days post fertilization (dpf) through 21 dpf, and gross cost of transport and factorial aerobic metabolic scope were derived from [Formula: see text] measurements. Heart rate generally ranged between 150 and 225 bpm in both L(HSD) and L(LSD) populations. However, significant (P < 0.05) differences existed between the L(LSD) and L(HSD) populations at 5 and 14 dpf in f(H),r and at days 10 and 15 dpf in f(H),a. [Formula: see text] was 0.04-0.32 μmol mg(-1) h(-1), while [Formula: see text] was 0.2-1.2 μmol mg(-1) h(-1). Significant (P < 0.05) differences between the L(LSD) and L(HSD) populations in [Formula: see text] occurred at 7, 10, and 21 dpf and in [Formula: see text] at 7 dpf. Gross cost of transport was ∼6-10 μmol O(2)·μg(-1) m(-1) at 5 dpf, peaking at 14-19 μmol O(2) μg(-1) m(-1) at 7-10 dpf, before falling again to 5-6 μmol O(2) μg(-1) m(-1) at 21 dpf, with gross cost of transport significantly higher in the L(LSD) population at 7 dpf. Collectively, these data indicate that inherited physiological differences known to contribute to enhanced stamina in adult parents also appear in their larval offspring well before attainment of juvenile or adult features.

No MeSH data available.


Related in: MedlinePlus