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The effect of contrast medium SonoVue® on the electric charge density of blood cells.

Petelska AD, Janica JR, Kotynska J, Łebkowska U, Figaszewski ZA - J. Membr. Biol. (2011)

Bottom Line: The effect of contrast medium SonoVue® on the electric charge density of blood cells (erythrocytes and thrombocytes) was measured using a microelectrophoretic method.Surface charge density values were determined from electrophoretic mobility measurements of blood cells performed at various pH levels.The deviation observed at a higher pH may be caused by disregarding interactions between the functional groups of blood cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University in Bialystok, Al. J. Pilsudskiego 11/4, 15-443 Bialystok, Poland. aneta@uwb.edu.pl

ABSTRACT
The effect of contrast medium SonoVue® on the electric charge density of blood cells (erythrocytes and thrombocytes) was measured using a microelectrophoretic method. We examined the effect of adsorbed H⁺ and OH⁻ ions on the surface charge of erythrocytes or thrombocytes. Surface charge density values were determined from electrophoretic mobility measurements of blood cells performed at various pH levels. The interaction between solution ions and the erythrocyte's or thrombocyte's surface was described by a four-component equilibrium model. The agreement between the experimental and theoretical charge variation curves of the erythrocytes and thrombocytes was good at pH 2-9. The deviation observed at a higher pH may be caused by disregarding interactions between the functional groups of blood cells.

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pH dependence of surface charge density of erythrocytes: filled circle control; open square SonoVue effect (experimental values are indicated by points and theoretical values, by the curve)
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Fig2: pH dependence of surface charge density of erythrocytes: filled circle control; open square SonoVue effect (experimental values are indicated by points and theoretical values, by the curve)

Mentions: The electrophoretic mobility measurements of the blood cells containing SonoVue formed the basis for investigations of ion/membrane association phenomena. Experiments were performed at several pH values using 0.155 M NaCl as a supporting electrolyte. The electrophoretic mobility values were converted to surface charge density using Eq. 17. The theoretical values of the surface charge density were determined by applying Eq. 12 to the experimental data. The association constants of blood cell surface groups with Na+ and Cl− [ = 0.230 (m3/mol),  = 0.076 (m3/mol)] have been previously reported (Dobrzynska et al. 2007). The calculated association constants were substituted into Eq. 12 to produce a theoretical curve. The surface charge densities of the control and SonoVue erythrocytes are plotted as a function of pH in Fig. 2. The points denote the experimental values, and the continuous lines represent the theoretical values obtained from Eq. 12. The theoretical and experimental surface charge density values agree between pH 2 and 9 but diverge slightly in the high pH range. Deviations from the theoretical curve may be caused by interactions between the functional groups of the blood cell components.Fig. 2


The effect of contrast medium SonoVue® on the electric charge density of blood cells.

Petelska AD, Janica JR, Kotynska J, Łebkowska U, Figaszewski ZA - J. Membr. Biol. (2011)

pH dependence of surface charge density of erythrocytes: filled circle control; open square SonoVue effect (experimental values are indicated by points and theoretical values, by the curve)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3285753&req=5

Fig2: pH dependence of surface charge density of erythrocytes: filled circle control; open square SonoVue effect (experimental values are indicated by points and theoretical values, by the curve)
Mentions: The electrophoretic mobility measurements of the blood cells containing SonoVue formed the basis for investigations of ion/membrane association phenomena. Experiments were performed at several pH values using 0.155 M NaCl as a supporting electrolyte. The electrophoretic mobility values were converted to surface charge density using Eq. 17. The theoretical values of the surface charge density were determined by applying Eq. 12 to the experimental data. The association constants of blood cell surface groups with Na+ and Cl− [ = 0.230 (m3/mol),  = 0.076 (m3/mol)] have been previously reported (Dobrzynska et al. 2007). The calculated association constants were substituted into Eq. 12 to produce a theoretical curve. The surface charge densities of the control and SonoVue erythrocytes are plotted as a function of pH in Fig. 2. The points denote the experimental values, and the continuous lines represent the theoretical values obtained from Eq. 12. The theoretical and experimental surface charge density values agree between pH 2 and 9 but diverge slightly in the high pH range. Deviations from the theoretical curve may be caused by interactions between the functional groups of the blood cell components.Fig. 2

Bottom Line: The effect of contrast medium SonoVue® on the electric charge density of blood cells (erythrocytes and thrombocytes) was measured using a microelectrophoretic method.Surface charge density values were determined from electrophoretic mobility measurements of blood cells performed at various pH levels.The deviation observed at a higher pH may be caused by disregarding interactions between the functional groups of blood cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University in Bialystok, Al. J. Pilsudskiego 11/4, 15-443 Bialystok, Poland. aneta@uwb.edu.pl

ABSTRACT
The effect of contrast medium SonoVue® on the electric charge density of blood cells (erythrocytes and thrombocytes) was measured using a microelectrophoretic method. We examined the effect of adsorbed H⁺ and OH⁻ ions on the surface charge of erythrocytes or thrombocytes. Surface charge density values were determined from electrophoretic mobility measurements of blood cells performed at various pH levels. The interaction between solution ions and the erythrocyte's or thrombocyte's surface was described by a four-component equilibrium model. The agreement between the experimental and theoretical charge variation curves of the erythrocytes and thrombocytes was good at pH 2-9. The deviation observed at a higher pH may be caused by disregarding interactions between the functional groups of blood cells.

Show MeSH
Related in: MedlinePlus