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Identification of genome-wide variations among three elite restorer lines for hybrid-rice.

Li S, Wang S, Deng Q, Zheng A, Zhu J, Liu H, Wang L, Gao F, Zou T, Huang B, Cao X, Xu L, Yu C, Ai P, Li P - PLoS ONE (2012)

Bottom Line: With the genomic sequence of the Indica cultivar 9311 as the reference, the following genetic features were identified: 267,383 single-nucleotide polymorphisms (SNPs), 52,847 insertion/deletion polymorphisms (InDels), and 3,286 structural variations (SVs) in the genome of IR24; 288,764 SNPs, 59,658 InDels, and 3,226 SVs in MH63; and 259,862 SNPs, 55,500 InDels, and 3,127 SVs in SH527.Furthermore, variations in several important genes were also surveyed by alignment analysis in these lines.Our results suggest that genetic variations among these lines, although far lower than those reported in the landrace population, are greater than expected, indicating a complicated genetic basis for the phenotypic diversity of the restorer lines.

View Article: PubMed Central - PubMed

Affiliation: Rice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, China.

ABSTRACT
Rice restorer lines play an important role in three-line hybrid rice production. Previous research based on molecular tagging has suggested that the restorer lines used widely today have narrow genetic backgrounds. However, patterns of genetic variation at a genome-wide scale in these restorer lines remain largely unknown. The present study performed re-sequencing and genome-wide variation analysis of three important representative restorer lines, namely, IR24, MH63, and SH527, using the Solexa sequencing technology. With the genomic sequence of the Indica cultivar 9311 as the reference, the following genetic features were identified: 267,383 single-nucleotide polymorphisms (SNPs), 52,847 insertion/deletion polymorphisms (InDels), and 3,286 structural variations (SVs) in the genome of IR24; 288,764 SNPs, 59,658 InDels, and 3,226 SVs in MH63; and 259,862 SNPs, 55,500 InDels, and 3,127 SVs in SH527. Variations between samples were also determined by comparative analysis of authentic collections of SNPs, InDels, and SVs, and were functionally annotated. Furthermore, variations in several important genes were also surveyed by alignment analysis in these lines. Our results suggest that genetic variations among these lines, although far lower than those reported in the landrace population, are greater than expected, indicating a complicated genetic basis for the phenotypic diversity of the restorer lines. Identification of genome-wide variation and pattern analysis among the restorer lines will facilitate future genetic studies and the molecular improvement of hybrid rice.

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Top 10 GOs of InDels detected between samples.
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pone-0030952-g006: Top 10 GOs of InDels detected between samples.

Mentions: GO and PFAM analyses were further carried out for the shared and different SNPs (InDels) in genes between samples to explore gene functions. In both the shared and different SNPs (InDels), the top GOs were protein kinase activity, nucleic acid binding, protein binding, DNA binding, and catalytic activity (Fig. 5 and 6). Genes coding for leucine-rich repeats and NB-ARC domains were found to have a significantly higher ratio of nonsynonymous-to-synonymous SNPs than average. As these domains are common in proteins that mediate disease resistance in plants, our finding is consistent with these proteins being particularly diverse due to pathogen pressure.


Identification of genome-wide variations among three elite restorer lines for hybrid-rice.

Li S, Wang S, Deng Q, Zheng A, Zhu J, Liu H, Wang L, Gao F, Zou T, Huang B, Cao X, Xu L, Yu C, Ai P, Li P - PLoS ONE (2012)

Top 10 GOs of InDels detected between samples.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3285608&req=5

pone-0030952-g006: Top 10 GOs of InDels detected between samples.
Mentions: GO and PFAM analyses were further carried out for the shared and different SNPs (InDels) in genes between samples to explore gene functions. In both the shared and different SNPs (InDels), the top GOs were protein kinase activity, nucleic acid binding, protein binding, DNA binding, and catalytic activity (Fig. 5 and 6). Genes coding for leucine-rich repeats and NB-ARC domains were found to have a significantly higher ratio of nonsynonymous-to-synonymous SNPs than average. As these domains are common in proteins that mediate disease resistance in plants, our finding is consistent with these proteins being particularly diverse due to pathogen pressure.

Bottom Line: With the genomic sequence of the Indica cultivar 9311 as the reference, the following genetic features were identified: 267,383 single-nucleotide polymorphisms (SNPs), 52,847 insertion/deletion polymorphisms (InDels), and 3,286 structural variations (SVs) in the genome of IR24; 288,764 SNPs, 59,658 InDels, and 3,226 SVs in MH63; and 259,862 SNPs, 55,500 InDels, and 3,127 SVs in SH527.Furthermore, variations in several important genes were also surveyed by alignment analysis in these lines.Our results suggest that genetic variations among these lines, although far lower than those reported in the landrace population, are greater than expected, indicating a complicated genetic basis for the phenotypic diversity of the restorer lines.

View Article: PubMed Central - PubMed

Affiliation: Rice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, China.

ABSTRACT
Rice restorer lines play an important role in three-line hybrid rice production. Previous research based on molecular tagging has suggested that the restorer lines used widely today have narrow genetic backgrounds. However, patterns of genetic variation at a genome-wide scale in these restorer lines remain largely unknown. The present study performed re-sequencing and genome-wide variation analysis of three important representative restorer lines, namely, IR24, MH63, and SH527, using the Solexa sequencing technology. With the genomic sequence of the Indica cultivar 9311 as the reference, the following genetic features were identified: 267,383 single-nucleotide polymorphisms (SNPs), 52,847 insertion/deletion polymorphisms (InDels), and 3,286 structural variations (SVs) in the genome of IR24; 288,764 SNPs, 59,658 InDels, and 3,226 SVs in MH63; and 259,862 SNPs, 55,500 InDels, and 3,127 SVs in SH527. Variations between samples were also determined by comparative analysis of authentic collections of SNPs, InDels, and SVs, and were functionally annotated. Furthermore, variations in several important genes were also surveyed by alignment analysis in these lines. Our results suggest that genetic variations among these lines, although far lower than those reported in the landrace population, are greater than expected, indicating a complicated genetic basis for the phenotypic diversity of the restorer lines. Identification of genome-wide variation and pattern analysis among the restorer lines will facilitate future genetic studies and the molecular improvement of hybrid rice.

Show MeSH