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The use of nanoscale visible light-responsive photocatalyst TiO2-Pt for the elimination of soil-borne pathogens.

Chen YL, Chen YS, Chan H, Tseng YH, Yang SR, Tsai HY, Liu HY, Sun DS, Chang HH - PLoS ONE (2012)

Bottom Line: Unexpectedly, water containing up to 10% w/v dissolved soil particles did not reduce the antibacterial potency of TiO(2)-Pt, suggesting that the TiO(2)-Pt photocatalyst is suitable for use in soil-contaminated environments.The TiO(2)-Pt photocatalyst exerted superior antibacterial activity against a broad spectrum of human pathogens, including B. pseudomallei and B. cenocepacia.Soil particles (<10% w/v) did not significantly reduce the antibacterial activity of TiO(2)-Pt in water.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, Taiwan.

ABSTRACT
Exposure to the soil-borne pathogens Burkholderia pseudomallei and Burkholderia cenocepacia can lead to severe infections and even mortality. These pathogens exhibit a high resistance to antibiotic treatments. In addition, no licensed vaccine is currently available. A nanoscale platinum-containing titania photocatalyst (TiO(2)-Pt) has been shown to have a superior visible light-responsive photocatalytic ability to degrade chemical contaminants like nitrogen oxides. The antibacterial activity of the catalyst and its potential use in soil pathogen control were evaluated. Using the plating method, we found that TiO(2)-Pt exerts superior antibacterial performance against Escherichia coli compared to other commercially available and laboratory prepared ultraviolet/visible light-responsive titania photocatalysts. TiO(2)-Pt-mediated photocatalysis also affectively eliminates the soil-borne bacteria B. pseudomallei and B. cenocepacia. An air pouch infection mouse model further revealed that TiO(2)-Pt-mediated photocatalysis could reduce the pathogenicity of both strains of bacteria. Unexpectedly, water containing up to 10% w/v dissolved soil particles did not reduce the antibacterial potency of TiO(2)-Pt, suggesting that the TiO(2)-Pt photocatalyst is suitable for use in soil-contaminated environments. The TiO(2)-Pt photocatalyst exerted superior antibacterial activity against a broad spectrum of human pathogens, including B. pseudomallei and B. cenocepacia. Soil particles (<10% w/v) did not significantly reduce the antibacterial activity of TiO(2)-Pt in water. These findings suggest that the TiO(2)-Pt photocatalyst may have potential applications in the development of bactericides for soil-borne pathogens.

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Antibacterial activity of nanoscale TiO2-Pt.The antibacterial activity of TiO2-Pt-mediated photocatalysis against E. coli is compared with other UV and visible light-responsive photocatalysts. The bacterial number (CFU) in the untreated groups was normalized to 100%. * P<0.05, ** P<0.01 and *** P<0.001, compared to the respective groups without light. n = 6 (3 experiments with 2 replicates). The data are presented as mean ± SD.
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pone-0031212-g001: Antibacterial activity of nanoscale TiO2-Pt.The antibacterial activity of TiO2-Pt-mediated photocatalysis against E. coli is compared with other UV and visible light-responsive photocatalysts. The bacterial number (CFU) in the untreated groups was normalized to 100%. * P<0.05, ** P<0.01 and *** P<0.001, compared to the respective groups without light. n = 6 (3 experiments with 2 replicates). The data are presented as mean ± SD.

Mentions: Under visible light illumination, the nanoscale TiO2-Pt samples exerted superior killing of E. coli[5], [10] compared to the commercially available BA-PW25 [28], [29] and carbon-containing TiO2 (C150, C200) [7], [9], [30] (Fig. 1, * P<0.5, ** P<0.01 and *** P<0.001, compared to the respective without light groups). The ultraviolet (UV) light-responsive photocatalyst (ST01) was used as a negative control because it does not respond to visible light illumination (Fig. 1, ST01 groups) [7], [27]. To further investigate the bactericidal spectrum of TiO2-Pt nanoparticles, various human pathogens, including different strains of the soil-borne bacteria B. pseudomallei and B. cenocepacia, were analyzed (Table 1). TiO2-Pt-mediated photocatalysis eliminated a wide spectrum of human pathogens. Among these pathogens, B. pseudomallei strains were the most susceptible to TiO2-Pt-mediated photocatalysis (Table 1, B. pseudomallei vgh07, vgh19, vgh21; 15–18% survival rates).


The use of nanoscale visible light-responsive photocatalyst TiO2-Pt for the elimination of soil-borne pathogens.

Chen YL, Chen YS, Chan H, Tseng YH, Yang SR, Tsai HY, Liu HY, Sun DS, Chang HH - PLoS ONE (2012)

Antibacterial activity of nanoscale TiO2-Pt.The antibacterial activity of TiO2-Pt-mediated photocatalysis against E. coli is compared with other UV and visible light-responsive photocatalysts. The bacterial number (CFU) in the untreated groups was normalized to 100%. * P<0.05, ** P<0.01 and *** P<0.001, compared to the respective groups without light. n = 6 (3 experiments with 2 replicates). The data are presented as mean ± SD.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3285157&req=5

pone-0031212-g001: Antibacterial activity of nanoscale TiO2-Pt.The antibacterial activity of TiO2-Pt-mediated photocatalysis against E. coli is compared with other UV and visible light-responsive photocatalysts. The bacterial number (CFU) in the untreated groups was normalized to 100%. * P<0.05, ** P<0.01 and *** P<0.001, compared to the respective groups without light. n = 6 (3 experiments with 2 replicates). The data are presented as mean ± SD.
Mentions: Under visible light illumination, the nanoscale TiO2-Pt samples exerted superior killing of E. coli[5], [10] compared to the commercially available BA-PW25 [28], [29] and carbon-containing TiO2 (C150, C200) [7], [9], [30] (Fig. 1, * P<0.5, ** P<0.01 and *** P<0.001, compared to the respective without light groups). The ultraviolet (UV) light-responsive photocatalyst (ST01) was used as a negative control because it does not respond to visible light illumination (Fig. 1, ST01 groups) [7], [27]. To further investigate the bactericidal spectrum of TiO2-Pt nanoparticles, various human pathogens, including different strains of the soil-borne bacteria B. pseudomallei and B. cenocepacia, were analyzed (Table 1). TiO2-Pt-mediated photocatalysis eliminated a wide spectrum of human pathogens. Among these pathogens, B. pseudomallei strains were the most susceptible to TiO2-Pt-mediated photocatalysis (Table 1, B. pseudomallei vgh07, vgh19, vgh21; 15–18% survival rates).

Bottom Line: Unexpectedly, water containing up to 10% w/v dissolved soil particles did not reduce the antibacterial potency of TiO(2)-Pt, suggesting that the TiO(2)-Pt photocatalyst is suitable for use in soil-contaminated environments.The TiO(2)-Pt photocatalyst exerted superior antibacterial activity against a broad spectrum of human pathogens, including B. pseudomallei and B. cenocepacia.Soil particles (<10% w/v) did not significantly reduce the antibacterial activity of TiO(2)-Pt in water.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, Taiwan.

ABSTRACT
Exposure to the soil-borne pathogens Burkholderia pseudomallei and Burkholderia cenocepacia can lead to severe infections and even mortality. These pathogens exhibit a high resistance to antibiotic treatments. In addition, no licensed vaccine is currently available. A nanoscale platinum-containing titania photocatalyst (TiO(2)-Pt) has been shown to have a superior visible light-responsive photocatalytic ability to degrade chemical contaminants like nitrogen oxides. The antibacterial activity of the catalyst and its potential use in soil pathogen control were evaluated. Using the plating method, we found that TiO(2)-Pt exerts superior antibacterial performance against Escherichia coli compared to other commercially available and laboratory prepared ultraviolet/visible light-responsive titania photocatalysts. TiO(2)-Pt-mediated photocatalysis also affectively eliminates the soil-borne bacteria B. pseudomallei and B. cenocepacia. An air pouch infection mouse model further revealed that TiO(2)-Pt-mediated photocatalysis could reduce the pathogenicity of both strains of bacteria. Unexpectedly, water containing up to 10% w/v dissolved soil particles did not reduce the antibacterial potency of TiO(2)-Pt, suggesting that the TiO(2)-Pt photocatalyst is suitable for use in soil-contaminated environments. The TiO(2)-Pt photocatalyst exerted superior antibacterial activity against a broad spectrum of human pathogens, including B. pseudomallei and B. cenocepacia. Soil particles (<10% w/v) did not significantly reduce the antibacterial activity of TiO(2)-Pt in water. These findings suggest that the TiO(2)-Pt photocatalyst may have potential applications in the development of bactericides for soil-borne pathogens.

Show MeSH
Related in: MedlinePlus