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Molecular epidemiology of livestock rabies viruses isolated in the northeastern Brazilian states of Paraíba and Pernambuco from 2003 - 2009.

Mochizuki N, Kawasaki H, Silva ML, Afonso JA, Itou T, Ito FH, Sakai T - BMC Res Notes (2012)

Bottom Line: Phylogenetic analysis based on 890 nt of the nucleoprotein (N) gene revealed that the 52 livestock-derived RABV isolates characterized here belonged to a single lineage.These isolates clustered with a vampire bat-related RABV lineage previously identified in other states in Brazil; within PB and PE, this lineage was divided between the previously characterized main lineage and a novel sub-lineage.This distribution pattern may correlate to that of a vampire bat population isolated by geographic barriers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Nihon University Veterinary Research Center, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. itou.takuya@nihon-u.ac.jp.

ABSTRACT

Background: Limited or no epidemiological information has been reported for rabies viruses (RABVs) isolated from livestock in the northeastern Brazilian states of Paraíba (PB) and Pernambuco (PE). The aim of this study was to clarify the molecular epidemiology of RABVs circulating in livestock, especially cattle, in these areas between 2003 and 2009.

Findings: Phylogenetic analysis based on 890 nt of the nucleoprotein (N) gene revealed that the 52 livestock-derived RABV isolates characterized here belonged to a single lineage. These isolates clustered with a vampire bat-related RABV lineage previously identified in other states in Brazil; within PB and PE, this lineage was divided between the previously characterized main lineage and a novel sub-lineage.

Conclusions: The occurrences of livestock rabies in PB and PE originated from vampire bat RABVs, and the causative RABV lineage has been circulating in this area of northeastern Brazil for at least 7 years. This distribution pattern may correlate to that of a vampire bat population isolated by geographic barriers.

No MeSH data available.


Related in: MedlinePlus

Geographic distribution of rabies virus isolates in the Brazilian states of Paraíba and Pernambuco. (a) Map of Brazil indicating location of states pertinent to this study. State abbreviations are as follows: PB, Paraíba; PE Pernambuco; GO, Goiás; SP, São Paulo; RJ, Rio de Janeiro; MT, Mato Grosso; TO, Tocantins; MA, Maranhão; PA, Pará; MS, Mato Grosso do Sul. (b, c) Detailed geographic distribution of livestock isolates classified as genetic variants in the states of PB (b) and PE (c). The two symbols (triangle and star) correspond to the new sub-lineage and previously reported lineage, respectively, as used in Figure 1. Samples for which the geographic origin and the genetic variant are identical are illustrated using the same symbol. Brazilian maps were obtained from Brasil em Relevo - Embrapa Monitoramento por Satélite http://www.relevobr.cnpm.embrapa.br/.
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Figure 2: Geographic distribution of rabies virus isolates in the Brazilian states of Paraíba and Pernambuco. (a) Map of Brazil indicating location of states pertinent to this study. State abbreviations are as follows: PB, Paraíba; PE Pernambuco; GO, Goiás; SP, São Paulo; RJ, Rio de Janeiro; MT, Mato Grosso; TO, Tocantins; MA, Maranhão; PA, Pará; MS, Mato Grosso do Sul. (b, c) Detailed geographic distribution of livestock isolates classified as genetic variants in the states of PB (b) and PE (c). The two symbols (triangle and star) correspond to the new sub-lineage and previously reported lineage, respectively, as used in Figure 1. Samples for which the geographic origin and the genetic variant are identical are illustrated using the same symbol. Brazilian maps were obtained from Brasil em Relevo - Embrapa Monitoramento por Satélite http://www.relevobr.cnpm.embrapa.br/.

Mentions: Phylogenetic analysis based on the sequences of 890 nt of the N gene revealed that the 52 RABV isolates included in this study clustered with a vampire bat-related RABV lineage; these isolates did not cluster with the dog-, fox-, or insectivorous bat-related RABVs (Figure 1). Comparison with RABVs isolated from other states in Brazil indicated that these 52 RABV isolates belonged to a single lineage; furthermore, this lineage was divided between a previously characterized main lineage (B) and a novel sub-lineage (A) consisting of several isolates located in PE. Geographical plotting showed that RABV isolates of the novel sub-lineage were derived from neighbouring areas (Figure 2). The topographical distributions of isolates from lineages A and B could not be distinguished in the areas covered by this study.


Molecular epidemiology of livestock rabies viruses isolated in the northeastern Brazilian states of Paraíba and Pernambuco from 2003 - 2009.

Mochizuki N, Kawasaki H, Silva ML, Afonso JA, Itou T, Ito FH, Sakai T - BMC Res Notes (2012)

Geographic distribution of rabies virus isolates in the Brazilian states of Paraíba and Pernambuco. (a) Map of Brazil indicating location of states pertinent to this study. State abbreviations are as follows: PB, Paraíba; PE Pernambuco; GO, Goiás; SP, São Paulo; RJ, Rio de Janeiro; MT, Mato Grosso; TO, Tocantins; MA, Maranhão; PA, Pará; MS, Mato Grosso do Sul. (b, c) Detailed geographic distribution of livestock isolates classified as genetic variants in the states of PB (b) and PE (c). The two symbols (triangle and star) correspond to the new sub-lineage and previously reported lineage, respectively, as used in Figure 1. Samples for which the geographic origin and the genetic variant are identical are illustrated using the same symbol. Brazilian maps were obtained from Brasil em Relevo - Embrapa Monitoramento por Satélite http://www.relevobr.cnpm.embrapa.br/.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3285087&req=5

Figure 2: Geographic distribution of rabies virus isolates in the Brazilian states of Paraíba and Pernambuco. (a) Map of Brazil indicating location of states pertinent to this study. State abbreviations are as follows: PB, Paraíba; PE Pernambuco; GO, Goiás; SP, São Paulo; RJ, Rio de Janeiro; MT, Mato Grosso; TO, Tocantins; MA, Maranhão; PA, Pará; MS, Mato Grosso do Sul. (b, c) Detailed geographic distribution of livestock isolates classified as genetic variants in the states of PB (b) and PE (c). The two symbols (triangle and star) correspond to the new sub-lineage and previously reported lineage, respectively, as used in Figure 1. Samples for which the geographic origin and the genetic variant are identical are illustrated using the same symbol. Brazilian maps were obtained from Brasil em Relevo - Embrapa Monitoramento por Satélite http://www.relevobr.cnpm.embrapa.br/.
Mentions: Phylogenetic analysis based on the sequences of 890 nt of the N gene revealed that the 52 RABV isolates included in this study clustered with a vampire bat-related RABV lineage; these isolates did not cluster with the dog-, fox-, or insectivorous bat-related RABVs (Figure 1). Comparison with RABVs isolated from other states in Brazil indicated that these 52 RABV isolates belonged to a single lineage; furthermore, this lineage was divided between a previously characterized main lineage (B) and a novel sub-lineage (A) consisting of several isolates located in PE. Geographical plotting showed that RABV isolates of the novel sub-lineage were derived from neighbouring areas (Figure 2). The topographical distributions of isolates from lineages A and B could not be distinguished in the areas covered by this study.

Bottom Line: Phylogenetic analysis based on 890 nt of the nucleoprotein (N) gene revealed that the 52 livestock-derived RABV isolates characterized here belonged to a single lineage.These isolates clustered with a vampire bat-related RABV lineage previously identified in other states in Brazil; within PB and PE, this lineage was divided between the previously characterized main lineage and a novel sub-lineage.This distribution pattern may correlate to that of a vampire bat population isolated by geographic barriers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Nihon University Veterinary Research Center, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. itou.takuya@nihon-u.ac.jp.

ABSTRACT

Background: Limited or no epidemiological information has been reported for rabies viruses (RABVs) isolated from livestock in the northeastern Brazilian states of Paraíba (PB) and Pernambuco (PE). The aim of this study was to clarify the molecular epidemiology of RABVs circulating in livestock, especially cattle, in these areas between 2003 and 2009.

Findings: Phylogenetic analysis based on 890 nt of the nucleoprotein (N) gene revealed that the 52 livestock-derived RABV isolates characterized here belonged to a single lineage. These isolates clustered with a vampire bat-related RABV lineage previously identified in other states in Brazil; within PB and PE, this lineage was divided between the previously characterized main lineage and a novel sub-lineage.

Conclusions: The occurrences of livestock rabies in PB and PE originated from vampire bat RABVs, and the causative RABV lineage has been circulating in this area of northeastern Brazil for at least 7 years. This distribution pattern may correlate to that of a vampire bat population isolated by geographic barriers.

No MeSH data available.


Related in: MedlinePlus