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SNPs in DNA repair or oxidative stress genes and late subcutaneous fibrosis in patients following single shot partial breast irradiation.

Falvo E, Strigari L, Citro G, Giordano C, Boboc G, Fabretti F, Bruzzaniti V, Bellesi L, Muti P, Blandino G, Pinnarò P - J. Exp. Clin. Cancer Res. (2012)

Bottom Line: A total of 57 breast cancer patients who underwent SSPBI were genotyped for SNPs (single nucleotide polymorphisms) in XRCC1, XRCC3, GST and RAD51 by Pyrosequencing technology.Univariate analysis (ORs and 95% CI) was performed to correlate SNPs with the risk of developing ≥ G2 fibrosis or fat necrosis.A higher significant risk of developing ≥ G2 fibrosis or fat necrosis in patients with: polymorphic variant GSTP1 (Ile105Val) (OR = 2.9; 95%CI, 0.88-10.14, p = 0.047).

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Pharmacokinetic/Pharmacogenomic, Regina Elena National Cancer Institute, Rome, Italy. falvo@ifo.it

ABSTRACT

Background: The aim of this study was to evaluate the potential association between single nucleotide polymorphisms related response to radiotherapy injury, such as genes related to DNA repair or enzymes involved in anti-oxidative activities. The paper aims to identify marker genes able to predict an increased risk of late toxicity studying our group of patients who underwent a Single Shot 3D-CRT PBI (SSPBI) after BCS (breast conserving surgery).

Methods: A total of 57 breast cancer patients who underwent SSPBI were genotyped for SNPs (single nucleotide polymorphisms) in XRCC1, XRCC3, GST and RAD51 by Pyrosequencing technology. Univariate analysis (ORs and 95% CI) was performed to correlate SNPs with the risk of developing ≥ G2 fibrosis or fat necrosis.

Results: A higher significant risk of developing ≥ G2 fibrosis or fat necrosis in patients with: polymorphic variant GSTP1 (Ile105Val) (OR = 2.9; 95%CI, 0.88-10.14, p = 0.047).

Conclusions: The presence of some SNPs involved in DNA repair or response to oxidative stress seem to be able to predict late toxicity.

Trial registration: ClinicalTrials.gov: NCT01316328.

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Related in: MedlinePlus

Polymorphism distribution.
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Figure 1: Polymorphism distribution.

Mentions: To these study purposes, i.e. determining polymorphisms predicting late toxicity, we recruited 57 patients treated with SSPBI from March 2006 to January 2008. Out of 57 patients, 15 (26%) were also treated with adjuvant non-concomitant chemotherapy. The adjuvant chemotherapy had been completed 3-4 weeks before RT with the exception of one patient (underwent chemotherapy one-week after SSAPBI). Adjuvant hormonotherapy, as indicated, was given simultaneously with SSPBI. Patient, tumour and treatment related characteristics are listed in Table 1, respectively. In Table 2, we reported the abbreviations for the polymorphic sites. The genotyping procedure was successful in 57 patients. The observed allele frequencies of the polymorphic genes analyzed were comparable to those reported for European populations in the dbSNP database and are shown in Figure 1.


SNPs in DNA repair or oxidative stress genes and late subcutaneous fibrosis in patients following single shot partial breast irradiation.

Falvo E, Strigari L, Citro G, Giordano C, Boboc G, Fabretti F, Bruzzaniti V, Bellesi L, Muti P, Blandino G, Pinnarò P - J. Exp. Clin. Cancer Res. (2012)

Polymorphism distribution.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3285050&req=5

Figure 1: Polymorphism distribution.
Mentions: To these study purposes, i.e. determining polymorphisms predicting late toxicity, we recruited 57 patients treated with SSPBI from March 2006 to January 2008. Out of 57 patients, 15 (26%) were also treated with adjuvant non-concomitant chemotherapy. The adjuvant chemotherapy had been completed 3-4 weeks before RT with the exception of one patient (underwent chemotherapy one-week after SSAPBI). Adjuvant hormonotherapy, as indicated, was given simultaneously with SSPBI. Patient, tumour and treatment related characteristics are listed in Table 1, respectively. In Table 2, we reported the abbreviations for the polymorphic sites. The genotyping procedure was successful in 57 patients. The observed allele frequencies of the polymorphic genes analyzed were comparable to those reported for European populations in the dbSNP database and are shown in Figure 1.

Bottom Line: A total of 57 breast cancer patients who underwent SSPBI were genotyped for SNPs (single nucleotide polymorphisms) in XRCC1, XRCC3, GST and RAD51 by Pyrosequencing technology.Univariate analysis (ORs and 95% CI) was performed to correlate SNPs with the risk of developing ≥ G2 fibrosis or fat necrosis.A higher significant risk of developing ≥ G2 fibrosis or fat necrosis in patients with: polymorphic variant GSTP1 (Ile105Val) (OR = 2.9; 95%CI, 0.88-10.14, p = 0.047).

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Pharmacokinetic/Pharmacogenomic, Regina Elena National Cancer Institute, Rome, Italy. falvo@ifo.it

ABSTRACT

Background: The aim of this study was to evaluate the potential association between single nucleotide polymorphisms related response to radiotherapy injury, such as genes related to DNA repair or enzymes involved in anti-oxidative activities. The paper aims to identify marker genes able to predict an increased risk of late toxicity studying our group of patients who underwent a Single Shot 3D-CRT PBI (SSPBI) after BCS (breast conserving surgery).

Methods: A total of 57 breast cancer patients who underwent SSPBI were genotyped for SNPs (single nucleotide polymorphisms) in XRCC1, XRCC3, GST and RAD51 by Pyrosequencing technology. Univariate analysis (ORs and 95% CI) was performed to correlate SNPs with the risk of developing ≥ G2 fibrosis or fat necrosis.

Results: A higher significant risk of developing ≥ G2 fibrosis or fat necrosis in patients with: polymorphic variant GSTP1 (Ile105Val) (OR = 2.9; 95%CI, 0.88-10.14, p = 0.047).

Conclusions: The presence of some SNPs involved in DNA repair or response to oxidative stress seem to be able to predict late toxicity.

Trial registration: ClinicalTrials.gov: NCT01316328.

Show MeSH
Related in: MedlinePlus