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Application of hydrocyclones for continuous cultivation of SP-2/0 cells in perfusion bioreactors: Effect of hydrocyclone operating pressure.

Elsayed EA, Wagner R - BMC Proc (2011)

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Affiliation: Advanced Chair for Proteomics & Cytomics Research, Zoology Department, Faculty of Science, King Saud University, 11451 Riyadh, Kingdom of Saudi Arabia ; Natural & Microbial Products Dept., National Research Centre, Dokki, Cairo, Egypt.

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Hydrocyclones (HCs) have been recently extensively evaluated for their application in the separation of mammalian cells in perfusion bioreactors... Cell were cultured on serum-free ZKT-1 medium supplemented with 5% foetal bovine serum... The results obtained (Figure 1) showed that cells required an adaptation phase to adapt themselves to the HC operating pressure... Moreover, the length of the adaptation phase depends on the HC pressure... Generally, cells were able to grow with high viability after the operation of hydrocyclone at both tested pressure values (0.85 and 1.30 bar)... Maximal cell concentrations of about 8.2 × 10 and 6.0 × 10 mL were achieved at an operating pressure of 0.85 and 1.30 bar with a viability range from 92 to 98%, respectively... This corresponds to a 5- and 3.5-fold increase than the batch cultivation, respectively... Results (Figure 2) showed that increasing operating pressure from 0.85 to 1.30 bar increased the average total separation efficiency from 89 to 95%... Additionally, results also showed that hydrocyclone preferably separates more viable cells in the underflow, and hence, back to the bioreactor system... Thus, more dead cells are separated in the overflow leaving the bioreactor system, which will improve system viability and product quality. - Hydrocyclone can be successfully used for cell separation in continuous mammalian perfusion bioreactors. - Cells adapt themselves to the shear forces inside the HC without being adversely affected by the pressure. - Higher separation efficiencies up to 96% can be achieved depending on the operating HC pressure. - HCs separate preferably more living cells in the underflow, thus more dead cells are leaving the system, which will finally lead to the improvement of system viability and product quality.

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Hydrocyclone separation efficiency
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Figure 2: Hydrocyclone separation efficiency

Mentions: Results (Figure 2) showed that increasing operating pressure from 0.85 to 1.30 bar increased the average total separation efficiency from 89 to 95%. Additionally, results also showed that hydrocyclone preferably separates more viable cells in the underflow, and hence, back to the bioreactor system. Thus, more dead cells are separated in the overflow leaving the bioreactor system, which will improve system viability and product quality.


Application of hydrocyclones for continuous cultivation of SP-2/0 cells in perfusion bioreactors: Effect of hydrocyclone operating pressure.

Elsayed EA, Wagner R - BMC Proc (2011)

Hydrocyclone separation efficiency
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3285022&req=5

Figure 2: Hydrocyclone separation efficiency
Mentions: Results (Figure 2) showed that increasing operating pressure from 0.85 to 1.30 bar increased the average total separation efficiency from 89 to 95%. Additionally, results also showed that hydrocyclone preferably separates more viable cells in the underflow, and hence, back to the bioreactor system. Thus, more dead cells are separated in the overflow leaving the bioreactor system, which will improve system viability and product quality.

View Article: PubMed Central - HTML - PubMed

Affiliation: Advanced Chair for Proteomics & Cytomics Research, Zoology Department, Faculty of Science, King Saud University, 11451 Riyadh, Kingdom of Saudi Arabia ; Natural & Microbial Products Dept., National Research Centre, Dokki, Cairo, Egypt.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Hydrocyclones (HCs) have been recently extensively evaluated for their application in the separation of mammalian cells in perfusion bioreactors... Cell were cultured on serum-free ZKT-1 medium supplemented with 5% foetal bovine serum... The results obtained (Figure 1) showed that cells required an adaptation phase to adapt themselves to the HC operating pressure... Moreover, the length of the adaptation phase depends on the HC pressure... Generally, cells were able to grow with high viability after the operation of hydrocyclone at both tested pressure values (0.85 and 1.30 bar)... Maximal cell concentrations of about 8.2 × 10 and 6.0 × 10 mL were achieved at an operating pressure of 0.85 and 1.30 bar with a viability range from 92 to 98%, respectively... This corresponds to a 5- and 3.5-fold increase than the batch cultivation, respectively... Results (Figure 2) showed that increasing operating pressure from 0.85 to 1.30 bar increased the average total separation efficiency from 89 to 95%... Additionally, results also showed that hydrocyclone preferably separates more viable cells in the underflow, and hence, back to the bioreactor system... Thus, more dead cells are separated in the overflow leaving the bioreactor system, which will improve system viability and product quality. - Hydrocyclone can be successfully used for cell separation in continuous mammalian perfusion bioreactors. - Cells adapt themselves to the shear forces inside the HC without being adversely affected by the pressure. - Higher separation efficiencies up to 96% can be achieved depending on the operating HC pressure. - HCs separate preferably more living cells in the underflow, thus more dead cells are leaving the system, which will finally lead to the improvement of system viability and product quality.

No MeSH data available.


Related in: MedlinePlus