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The neuroprotective effects of electrolyzed reduced water and its model water containing molecular hydrogen and Pt nanoparticles.

Yan H, Kashiwaki T, Hamasaki T, Kinjo T, Teruya K, Kabayama S, Shirahata S - BMC Proc (2011)

View Article: PubMed Central - HTML - PubMed

Affiliation: Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

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Molecular hydrogen could scavenge ROS and protected brain from oxidative stress... In this research, we used TI-200S ERW derived from 2 mM NaOH solution produced by a batch type electrolysis device and model waters containing molecular hydrogen and synthetic Pt NPs of 2-3 nm sizes as research models of ERW to examine the anti-oxidant capabilities of ERW on several kinds of neural cells such as PC12, N1E115, and serum free mouse embryo (SFME) cells... We pretreated the ERW and 200 μM H2O2 and examined the neuroprotective effects of ERW on PC12, N1E115 and SFME cells, using WST-8 method... We also examined the ATP production of SFME cells after pretreated with ERW and H2O2 by Bioluminescence Assay Kit... Finally, we used dissolved hydrogen (DH) and Pt NPs as research models to examine their neuroprotective effects... ERW significantly reduced the cell death induced by H2O2 pretreatment (Figure 1)... ERW also scavenged the intracellular ROS and prevented the decrease of mitochondrial membrane potential and ATP production induced by ROS... We also examined the neuroprotective effects of molecular hydrogen and Pt NPs and showed that both molecular hydrogen and Pt NPs contributed to the neuroprotective effects of ERW... The results suggest that ERW is beneficial for the prevention and alleviation of oxidative stress-induced human neurodegenerative diseases.

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Protective effect of ERW on H2O2-induced neuroblastoma N1E115 cells death. Cells were treated with water samples (ERW and control ultrapure water with same pH with ERW) and 200 μM H2O2 for 24 h. Cell viabilities were assayed by WST-8 method. N=3, * p < 0.05.
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Figure 1: Protective effect of ERW on H2O2-induced neuroblastoma N1E115 cells death. Cells were treated with water samples (ERW and control ultrapure water with same pH with ERW) and 200 μM H2O2 for 24 h. Cell viabilities were assayed by WST-8 method. N=3, * p < 0.05.

Mentions: ERW significantly reduced the cell death induced by H2O2 pretreatment (Figure 1). ERW also scavenged the intracellular ROS and prevented the decrease of mitochondrial membrane potential and ATP production induced by ROS. We also examined the neuroprotective effects of molecular hydrogen and Pt NPs and showed that both molecular hydrogen and Pt NPs contributed to the neuroprotective effects of ERW.


The neuroprotective effects of electrolyzed reduced water and its model water containing molecular hydrogen and Pt nanoparticles.

Yan H, Kashiwaki T, Hamasaki T, Kinjo T, Teruya K, Kabayama S, Shirahata S - BMC Proc (2011)

Protective effect of ERW on H2O2-induced neuroblastoma N1E115 cells death. Cells were treated with water samples (ERW and control ultrapure water with same pH with ERW) and 200 μM H2O2 for 24 h. Cell viabilities were assayed by WST-8 method. N=3, * p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3285010&req=5

Figure 1: Protective effect of ERW on H2O2-induced neuroblastoma N1E115 cells death. Cells were treated with water samples (ERW and control ultrapure water with same pH with ERW) and 200 μM H2O2 for 24 h. Cell viabilities were assayed by WST-8 method. N=3, * p < 0.05.
Mentions: ERW significantly reduced the cell death induced by H2O2 pretreatment (Figure 1). ERW also scavenged the intracellular ROS and prevented the decrease of mitochondrial membrane potential and ATP production induced by ROS. We also examined the neuroprotective effects of molecular hydrogen and Pt NPs and showed that both molecular hydrogen and Pt NPs contributed to the neuroprotective effects of ERW.

View Article: PubMed Central - HTML - PubMed

Affiliation: Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Molecular hydrogen could scavenge ROS and protected brain from oxidative stress... In this research, we used TI-200S ERW derived from 2 mM NaOH solution produced by a batch type electrolysis device and model waters containing molecular hydrogen and synthetic Pt NPs of 2-3 nm sizes as research models of ERW to examine the anti-oxidant capabilities of ERW on several kinds of neural cells such as PC12, N1E115, and serum free mouse embryo (SFME) cells... We pretreated the ERW and 200 μM H2O2 and examined the neuroprotective effects of ERW on PC12, N1E115 and SFME cells, using WST-8 method... We also examined the ATP production of SFME cells after pretreated with ERW and H2O2 by Bioluminescence Assay Kit... Finally, we used dissolved hydrogen (DH) and Pt NPs as research models to examine their neuroprotective effects... ERW significantly reduced the cell death induced by H2O2 pretreatment (Figure 1)... ERW also scavenged the intracellular ROS and prevented the decrease of mitochondrial membrane potential and ATP production induced by ROS... We also examined the neuroprotective effects of molecular hydrogen and Pt NPs and showed that both molecular hydrogen and Pt NPs contributed to the neuroprotective effects of ERW... The results suggest that ERW is beneficial for the prevention and alleviation of oxidative stress-induced human neurodegenerative diseases.

No MeSH data available.


Related in: MedlinePlus