Limits...
Trace glucose and lipid metabolism in high androgen and high-fat diet induced polycystic ovary syndrome rats.

Zhai HL, Wu H, Xu H, Weng P, Xia FZ, Chen Y, Lu YL - Reprod. Biol. Endocrinol. (2012)

Bottom Line: Ra of glucose and GNG increased significantly in the andronate+HFD rats.However, the Ra of glycerol was similar in the three groups.Andronate with HFD rat model showed ovarian and metabolic features of PCOS, significant increase in glucose Ra, GNG, and lipid profiles, as well as normal blood glucose levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Endocrinology and Metabolism Research Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.

ABSTRACT

Background: There is a high prevalence of diabetes mellitus (DM) and dyslipidemia in women with polycystic ovary syndrome (PCOS). The purpose of this study was to investigate the role of different metabolic pathways in the development of diabetes mellitus in high-androgen female mice fed with a high-fat diet.

Methods: Female Sprague-Dawley rats were divided into 3 groups: the control group(C), n = 10; the andronate-treated group (Andronate), n = 10 (treated with andronate, 1 mg/100 g body weight/day for 8 weeks); and the andronate-treated and high-fat diet group (Andronate+HFD), n = 10. The rate of glucose appearance (Ra of glucose), gluconeogenesis (GNG), and the rate of glycerol appearance (Ra of glycerol) were assessed with a stable isotope tracer. The serum sex hormone levels, insulin levels, glucose concentration, and the lipid profile were also measured.

Results: Compared with control group, both andronate-treated groups exhibited obesity with higher insulin concentrations (P < 0.05) but similar blood glucose concentrations. Of the two andronate-treated groups, the andronate+HFD group had the most serious insulin resistance (IR). Estrus cycles were completely acyclic, with polycystic ovaries and elevated serum lipid profiles in the andronate+HFD group (P < 0.05). Ra of glucose and GNG increased significantly in the andronate+HFD rats. However, the Ra of glycerol was similar in the three groups.

Conclusions: Andronate with HFD rat model showed ovarian and metabolic features of PCOS, significant increase in glucose Ra, GNG, and lipid profiles, as well as normal blood glucose levels. Therefore, aberrant IR, increased glucose Ra, GNG, and lipid metabolism may represent the early-stage of glucose and lipid kinetics disorder, thereby might be used as potential early-stage treatment targets for PCOS.

Show MeSH

Related in: MedlinePlus

Protocol of in vivo infusions of stable isotopes. This is the protocol of steady-state isotope infusion. The arrows indicate the times for blood draws and tissue sampling.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3278365&req=5

Figure 3: Protocol of in vivo infusions of stable isotopes. This is the protocol of steady-state isotope infusion. The arrows indicate the times for blood draws and tissue sampling.

Mentions: In this study, appearance of glucose and glycerol were traced by [6,6-2D]-glucose and [U-13 C]-glycerol, while gluconeogenesis (GNG) were accessed by [1,2,3-13 C]-glucose and [U-13 C]-glycerol, [6,6-2D]-glucose (2 μmol/kg/min) and [U-13 C]-glycerol (0.84 μmol/kg/min) were infused intravenously constantly, without priming, through the tail infusion line driven by an infusion pump (Harvard Apparatus, Holliston, MA, USA)for 90 min. This is defined as the basal period. During the last 10 min (80-90 min), arterial blood samples (0.5 ml each) were collected 5 min intervals from the tail arterial catheter. These samples were used for the quantitation of steady state glucose and glycerol metabolism. The rats were then euthanized by heart-opening under anesthesia with pentobarbital (50 mg/kg) in order to reduce blood elements in tissues. Ovaries were harvested swiftly. Plasma was prepared on site and saved at -80°C for later analysis. A flow chart of the study design is shown in Figure 3.


Trace glucose and lipid metabolism in high androgen and high-fat diet induced polycystic ovary syndrome rats.

Zhai HL, Wu H, Xu H, Weng P, Xia FZ, Chen Y, Lu YL - Reprod. Biol. Endocrinol. (2012)

Protocol of in vivo infusions of stable isotopes. This is the protocol of steady-state isotope infusion. The arrows indicate the times for blood draws and tissue sampling.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3278365&req=5

Figure 3: Protocol of in vivo infusions of stable isotopes. This is the protocol of steady-state isotope infusion. The arrows indicate the times for blood draws and tissue sampling.
Mentions: In this study, appearance of glucose and glycerol were traced by [6,6-2D]-glucose and [U-13 C]-glycerol, while gluconeogenesis (GNG) were accessed by [1,2,3-13 C]-glucose and [U-13 C]-glycerol, [6,6-2D]-glucose (2 μmol/kg/min) and [U-13 C]-glycerol (0.84 μmol/kg/min) were infused intravenously constantly, without priming, through the tail infusion line driven by an infusion pump (Harvard Apparatus, Holliston, MA, USA)for 90 min. This is defined as the basal period. During the last 10 min (80-90 min), arterial blood samples (0.5 ml each) were collected 5 min intervals from the tail arterial catheter. These samples were used for the quantitation of steady state glucose and glycerol metabolism. The rats were then euthanized by heart-opening under anesthesia with pentobarbital (50 mg/kg) in order to reduce blood elements in tissues. Ovaries were harvested swiftly. Plasma was prepared on site and saved at -80°C for later analysis. A flow chart of the study design is shown in Figure 3.

Bottom Line: Ra of glucose and GNG increased significantly in the andronate+HFD rats.However, the Ra of glycerol was similar in the three groups.Andronate with HFD rat model showed ovarian and metabolic features of PCOS, significant increase in glucose Ra, GNG, and lipid profiles, as well as normal blood glucose levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Endocrinology and Metabolism Research Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.

ABSTRACT

Background: There is a high prevalence of diabetes mellitus (DM) and dyslipidemia in women with polycystic ovary syndrome (PCOS). The purpose of this study was to investigate the role of different metabolic pathways in the development of diabetes mellitus in high-androgen female mice fed with a high-fat diet.

Methods: Female Sprague-Dawley rats were divided into 3 groups: the control group(C), n = 10; the andronate-treated group (Andronate), n = 10 (treated with andronate, 1 mg/100 g body weight/day for 8 weeks); and the andronate-treated and high-fat diet group (Andronate+HFD), n = 10. The rate of glucose appearance (Ra of glucose), gluconeogenesis (GNG), and the rate of glycerol appearance (Ra of glycerol) were assessed with a stable isotope tracer. The serum sex hormone levels, insulin levels, glucose concentration, and the lipid profile were also measured.

Results: Compared with control group, both andronate-treated groups exhibited obesity with higher insulin concentrations (P < 0.05) but similar blood glucose concentrations. Of the two andronate-treated groups, the andronate+HFD group had the most serious insulin resistance (IR). Estrus cycles were completely acyclic, with polycystic ovaries and elevated serum lipid profiles in the andronate+HFD group (P < 0.05). Ra of glucose and GNG increased significantly in the andronate+HFD rats. However, the Ra of glycerol was similar in the three groups.

Conclusions: Andronate with HFD rat model showed ovarian and metabolic features of PCOS, significant increase in glucose Ra, GNG, and lipid profiles, as well as normal blood glucose levels. Therefore, aberrant IR, increased glucose Ra, GNG, and lipid metabolism may represent the early-stage of glucose and lipid kinetics disorder, thereby might be used as potential early-stage treatment targets for PCOS.

Show MeSH
Related in: MedlinePlus