Limits...
Human cytomegalovirus tegument proteins (pp65, pp71, pp150, pp28).

Tomtishen JP - Virol. J. (2012)

Bottom Line: In individuals whose immune systems are immature or weakened, HCMV is a significant pathogen causing morbidity and mortality.A possible target for novel antiviral treatments is the HCMV proteins that localize to the tegument of the virion, since they play important roles in all stages of the viral life cycle, including, viral entry, gene expression, immune evasion, assembly, and egress.The most likely tegument protein candidates are pp65 (immune evasion), pp71 (gene expression), and pp150 and pp28 (assembly and egress).

View Article: PubMed Central - HTML - PubMed

Affiliation: Bucknell University, Cell Biology/Biochemistry Program, Lewisburg, PA 17837, USA. jpt015@bucknell.edu

ABSTRACT
Human cytomegalovirus (HCMV), a member of the Betaherpesvirinae sub-family of Herpesviridae family, is a widespread pathogen that infects a majority of the world's population by early adulthood. In individuals whose immune systems are immature or weakened, HCMV is a significant pathogen causing morbidity and mortality. There is no effective vaccine and only limited antiviral treatments against HCMV infection to date. A possible target for novel antiviral treatments is the HCMV proteins that localize to the tegument of the virion, since they play important roles in all stages of the viral life cycle, including, viral entry, gene expression, immune evasion, assembly, and egress. The most likely tegument protein candidates are pp65 (immune evasion), pp71 (gene expression), and pp150 and pp28 (assembly and egress). Although the subcellular localization of these proteins has been identified during HCMV infections in vitro, their localization patterns have not been determined when each protein is expressed individually in living cells. Thus, the objective of this review is elucidate the HCMV tegument as well as present current research findings concerning the subcellular localization of the tegument proteins pp65, pp71, pp150, and pp28 as fusions to one of several fluorescent proteins.

Show MeSH

Related in: MedlinePlus

Fluorescently labeled HCMV tegument protein constructs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3278345&req=5

Figure 2: Fluorescently labeled HCMV tegument protein constructs.

Mentions: In total, three variant fluorescent proteins (Cyan-blue, RFP (DsRed2)-red, GFP-green) were used to tag and label the tegument proteins. Pp65 was tagged with Cyan, pp71 with RFP, and pp150 and pp28 with GFP. The characterization of each fluorescent protein molecule can be seen in Table 1. Additionally, the location of the fluorescent tags on each protein can be seen in Figure 2.


Human cytomegalovirus tegument proteins (pp65, pp71, pp150, pp28).

Tomtishen JP - Virol. J. (2012)

Fluorescently labeled HCMV tegument protein constructs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3278345&req=5

Figure 2: Fluorescently labeled HCMV tegument protein constructs.
Mentions: In total, three variant fluorescent proteins (Cyan-blue, RFP (DsRed2)-red, GFP-green) were used to tag and label the tegument proteins. Pp65 was tagged with Cyan, pp71 with RFP, and pp150 and pp28 with GFP. The characterization of each fluorescent protein molecule can be seen in Table 1. Additionally, the location of the fluorescent tags on each protein can be seen in Figure 2.

Bottom Line: In individuals whose immune systems are immature or weakened, HCMV is a significant pathogen causing morbidity and mortality.A possible target for novel antiviral treatments is the HCMV proteins that localize to the tegument of the virion, since they play important roles in all stages of the viral life cycle, including, viral entry, gene expression, immune evasion, assembly, and egress.The most likely tegument protein candidates are pp65 (immune evasion), pp71 (gene expression), and pp150 and pp28 (assembly and egress).

View Article: PubMed Central - HTML - PubMed

Affiliation: Bucknell University, Cell Biology/Biochemistry Program, Lewisburg, PA 17837, USA. jpt015@bucknell.edu

ABSTRACT
Human cytomegalovirus (HCMV), a member of the Betaherpesvirinae sub-family of Herpesviridae family, is a widespread pathogen that infects a majority of the world's population by early adulthood. In individuals whose immune systems are immature or weakened, HCMV is a significant pathogen causing morbidity and mortality. There is no effective vaccine and only limited antiviral treatments against HCMV infection to date. A possible target for novel antiviral treatments is the HCMV proteins that localize to the tegument of the virion, since they play important roles in all stages of the viral life cycle, including, viral entry, gene expression, immune evasion, assembly, and egress. The most likely tegument protein candidates are pp65 (immune evasion), pp71 (gene expression), and pp150 and pp28 (assembly and egress). Although the subcellular localization of these proteins has been identified during HCMV infections in vitro, their localization patterns have not been determined when each protein is expressed individually in living cells. Thus, the objective of this review is elucidate the HCMV tegument as well as present current research findings concerning the subcellular localization of the tegument proteins pp65, pp71, pp150, and pp28 as fusions to one of several fluorescent proteins.

Show MeSH
Related in: MedlinePlus