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Human cytomegalovirus tegument proteins (pp65, pp71, pp150, pp28).

Tomtishen JP - Virol. J. (2012)

Bottom Line: In individuals whose immune systems are immature or weakened, HCMV is a significant pathogen causing morbidity and mortality.A possible target for novel antiviral treatments is the HCMV proteins that localize to the tegument of the virion, since they play important roles in all stages of the viral life cycle, including, viral entry, gene expression, immune evasion, assembly, and egress.The most likely tegument protein candidates are pp65 (immune evasion), pp71 (gene expression), and pp150 and pp28 (assembly and egress).

View Article: PubMed Central - HTML - PubMed

Affiliation: Bucknell University, Cell Biology/Biochemistry Program, Lewisburg, PA 17837, USA. jpt015@bucknell.edu

ABSTRACT
Human cytomegalovirus (HCMV), a member of the Betaherpesvirinae sub-family of Herpesviridae family, is a widespread pathogen that infects a majority of the world's population by early adulthood. In individuals whose immune systems are immature or weakened, HCMV is a significant pathogen causing morbidity and mortality. There is no effective vaccine and only limited antiviral treatments against HCMV infection to date. A possible target for novel antiviral treatments is the HCMV proteins that localize to the tegument of the virion, since they play important roles in all stages of the viral life cycle, including, viral entry, gene expression, immune evasion, assembly, and egress. The most likely tegument protein candidates are pp65 (immune evasion), pp71 (gene expression), and pp150 and pp28 (assembly and egress). Although the subcellular localization of these proteins has been identified during HCMV infections in vitro, their localization patterns have not been determined when each protein is expressed individually in living cells. Thus, the objective of this review is elucidate the HCMV tegument as well as present current research findings concerning the subcellular localization of the tegument proteins pp65, pp71, pp150, and pp28 as fusions to one of several fluorescent proteins.

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A cartoon depicting the structure of the HCMV virion. (Image obtained from (http://www.virology.net/big_virology/bvdnaherpes.html) courtesy of Dr. Marko Reschke in Marburg, Germany).
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Figure 1: A cartoon depicting the structure of the HCMV virion. (Image obtained from (http://www.virology.net/big_virology/bvdnaherpes.html) courtesy of Dr. Marko Reschke in Marburg, Germany).

Mentions: HCMV has the prototypical herpesvirus virion structure (Figure 1) and the replication cycle has a well controlled cascade of gene expression [9]. The virion has an icosahedral protein capsid that contains the 235-kb double-stranded DNA. The capsid is surrounded by a proteinaceous tegument and an outer lipid envelope [1]. Virions gain entry into a cell through a membrane fusion event involving the outer membrane of the cell and glycoproteins on the lipid envelope of virions. Once the fusion of these two membranes occurs, the DNA-containing protein capsid and the tegument proteins are released into the cell [11].


Human cytomegalovirus tegument proteins (pp65, pp71, pp150, pp28).

Tomtishen JP - Virol. J. (2012)

A cartoon depicting the structure of the HCMV virion. (Image obtained from (http://www.virology.net/big_virology/bvdnaherpes.html) courtesy of Dr. Marko Reschke in Marburg, Germany).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3278345&req=5

Figure 1: A cartoon depicting the structure of the HCMV virion. (Image obtained from (http://www.virology.net/big_virology/bvdnaherpes.html) courtesy of Dr. Marko Reschke in Marburg, Germany).
Mentions: HCMV has the prototypical herpesvirus virion structure (Figure 1) and the replication cycle has a well controlled cascade of gene expression [9]. The virion has an icosahedral protein capsid that contains the 235-kb double-stranded DNA. The capsid is surrounded by a proteinaceous tegument and an outer lipid envelope [1]. Virions gain entry into a cell through a membrane fusion event involving the outer membrane of the cell and glycoproteins on the lipid envelope of virions. Once the fusion of these two membranes occurs, the DNA-containing protein capsid and the tegument proteins are released into the cell [11].

Bottom Line: In individuals whose immune systems are immature or weakened, HCMV is a significant pathogen causing morbidity and mortality.A possible target for novel antiviral treatments is the HCMV proteins that localize to the tegument of the virion, since they play important roles in all stages of the viral life cycle, including, viral entry, gene expression, immune evasion, assembly, and egress.The most likely tegument protein candidates are pp65 (immune evasion), pp71 (gene expression), and pp150 and pp28 (assembly and egress).

View Article: PubMed Central - HTML - PubMed

Affiliation: Bucknell University, Cell Biology/Biochemistry Program, Lewisburg, PA 17837, USA. jpt015@bucknell.edu

ABSTRACT
Human cytomegalovirus (HCMV), a member of the Betaherpesvirinae sub-family of Herpesviridae family, is a widespread pathogen that infects a majority of the world's population by early adulthood. In individuals whose immune systems are immature or weakened, HCMV is a significant pathogen causing morbidity and mortality. There is no effective vaccine and only limited antiviral treatments against HCMV infection to date. A possible target for novel antiviral treatments is the HCMV proteins that localize to the tegument of the virion, since they play important roles in all stages of the viral life cycle, including, viral entry, gene expression, immune evasion, assembly, and egress. The most likely tegument protein candidates are pp65 (immune evasion), pp71 (gene expression), and pp150 and pp28 (assembly and egress). Although the subcellular localization of these proteins has been identified during HCMV infections in vitro, their localization patterns have not been determined when each protein is expressed individually in living cells. Thus, the objective of this review is elucidate the HCMV tegument as well as present current research findings concerning the subcellular localization of the tegument proteins pp65, pp71, pp150, and pp28 as fusions to one of several fluorescent proteins.

Show MeSH
Related in: MedlinePlus