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Level of 8-OHdG in drained dialysate appears to be a marker of peritoneal damage in peritoneal dialysis.

Morishita Y, Watanabe M, Hirahara I, Akimoto T, Muto S, Kusano E - Int J Nephrol Renovasc Dis (2011)

Bottom Line: Peritoneal dialysis (PD) is a successful renal replacement therapy; however, long-term PD leads to structural and functional peritoneal damage.The 8-OHdG level was significantly correlated with dialysate:plasma creatine ratio (r = 0.463, P < 0.05) and significantly inversely correlated with D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection) (r = -0.474, P < 0.05).The 8-OHdG level was also significantly correlated with MMP-2 level (r = 0.551, P < 0.05), but it was not correlated with the age of subjects, the duration of PD, or blood pressure.

View Article: PubMed Central - PubMed

Affiliation: Division of Nephrology, Department of Medicine, Jichi Medical University, Tochigi, Japan.

ABSTRACT

Purpose: Peritoneal dialysis (PD) is a successful renal replacement therapy; however, long-term PD leads to structural and functional peritoneal damage. Therefore, the monitoring and estimation of peritoneal function are important in PD patients. Oxidative stress has been implicated as one possible mechanism of peritoneal membrane damage. The aim of this study was to evaluate the association between an oxidative stress marker, 8-hydroxydeoxyguanosine (8-OHdG), and peritoneal damage in PD patients.

Methods: The authors evaluated 8-OHdG in drained dialysate by enzyme immunoassay to investigate the association between 8-OHdG and solute transport rate estimated by peritoneal equilibration test and matrix metalloproteinase-2 (MMP-2) level in 45 samples from 28 PD patients.

Results: The 8-OHdG level was significantly correlated with dialysate:plasma creatine ratio (r = 0.463, P < 0.05) and significantly inversely correlated with D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection) (r = -0.474, P < 0.05). The 8-OHdG level was also significantly correlated with MMP-2 level (r = 0.551, P < 0.05), but it was not correlated with the age of subjects, the duration of PD, or blood pressure.

Conclusion: The level of 8-OHdG in drained dialysate may be a useful novel marker of peritoneal damage in PD.

No MeSH data available.


Related in: MedlinePlus

Correlation between the 8-hydroxydeoxyguanosine (8-OHdG) level in drained dialysate and the peritoneal solute transport rate, determined by peritoneal equilibration test and matrix metalloproteinase-2 (MMP-2) level in drained dialysate: 8-OHdG level and (A) dialysate:plasma creatine ratio (D/P Cr), D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection), and MMP-2 level in peritoneal dialysis (PD) patients and (B) D/P Cr, D/D0 glucose, and MMP-2 level in those PD patients who had a past history of peritonitis and in those PD patients who did not.
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f2-ijnrd-5-009: Correlation between the 8-hydroxydeoxyguanosine (8-OHdG) level in drained dialysate and the peritoneal solute transport rate, determined by peritoneal equilibration test and matrix metalloproteinase-2 (MMP-2) level in drained dialysate: 8-OHdG level and (A) dialysate:plasma creatine ratio (D/P Cr), D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection), and MMP-2 level in peritoneal dialysis (PD) patients and (B) D/P Cr, D/D0 glucose, and MMP-2 level in those PD patients who had a past history of peritonitis and in those PD patients who did not.

Mentions: The 8-OHdG level in the drained dialysate was significantly correlated with D/P Cr (r = 0.463, P < 0.05) and significantly inversely correlated with D/D0 glucose (r = −0.474, P < 0.05) in PD patients (Figure 2A). The 8-OHdG level in the drained dialysate was also significantly correlated with MMP-2 level in the drained dialysate (r = 0.551, P < 0.05) (Figure 2A). In the subanalysis based on past history of peritonitis, 8-OHdG level in the drained dialysate correlated with D/P Cr and inversely correlated with D/D0 glucose in both the PD patients who had a past history of peritonitis (D/P Cr: r = 0.474, P < 0.05; D/D0 glucose: r = −0.646, P < 0.05) and the PD patients who did not have a past history of peritonitis (D/P Cr: r = 0.420, P < 0.05; D/D0 glucose: r = −0.376, P < 0.05) (Figure 2B). On the other hand, 8-OHdG level in the drained dialysate was significantly correlated with MMP-2 level in drained dialysate in the PD patients who had a past history of peritonitis (r = 0.730, P < 0.05); however, it was not correlated with MMP-2 level in drained dialysate in those PD patients who did not have past history of peritonitis (Figure 2B).


Level of 8-OHdG in drained dialysate appears to be a marker of peritoneal damage in peritoneal dialysis.

Morishita Y, Watanabe M, Hirahara I, Akimoto T, Muto S, Kusano E - Int J Nephrol Renovasc Dis (2011)

Correlation between the 8-hydroxydeoxyguanosine (8-OHdG) level in drained dialysate and the peritoneal solute transport rate, determined by peritoneal equilibration test and matrix metalloproteinase-2 (MMP-2) level in drained dialysate: 8-OHdG level and (A) dialysate:plasma creatine ratio (D/P Cr), D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection), and MMP-2 level in peritoneal dialysis (PD) patients and (B) D/P Cr, D/D0 glucose, and MMP-2 level in those PD patients who had a past history of peritonitis and in those PD patients who did not.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3278254&req=5

f2-ijnrd-5-009: Correlation between the 8-hydroxydeoxyguanosine (8-OHdG) level in drained dialysate and the peritoneal solute transport rate, determined by peritoneal equilibration test and matrix metalloproteinase-2 (MMP-2) level in drained dialysate: 8-OHdG level and (A) dialysate:plasma creatine ratio (D/P Cr), D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection), and MMP-2 level in peritoneal dialysis (PD) patients and (B) D/P Cr, D/D0 glucose, and MMP-2 level in those PD patients who had a past history of peritonitis and in those PD patients who did not.
Mentions: The 8-OHdG level in the drained dialysate was significantly correlated with D/P Cr (r = 0.463, P < 0.05) and significantly inversely correlated with D/D0 glucose (r = −0.474, P < 0.05) in PD patients (Figure 2A). The 8-OHdG level in the drained dialysate was also significantly correlated with MMP-2 level in the drained dialysate (r = 0.551, P < 0.05) (Figure 2A). In the subanalysis based on past history of peritonitis, 8-OHdG level in the drained dialysate correlated with D/P Cr and inversely correlated with D/D0 glucose in both the PD patients who had a past history of peritonitis (D/P Cr: r = 0.474, P < 0.05; D/D0 glucose: r = −0.646, P < 0.05) and the PD patients who did not have a past history of peritonitis (D/P Cr: r = 0.420, P < 0.05; D/D0 glucose: r = −0.376, P < 0.05) (Figure 2B). On the other hand, 8-OHdG level in the drained dialysate was significantly correlated with MMP-2 level in drained dialysate in the PD patients who had a past history of peritonitis (r = 0.730, P < 0.05); however, it was not correlated with MMP-2 level in drained dialysate in those PD patients who did not have past history of peritonitis (Figure 2B).

Bottom Line: Peritoneal dialysis (PD) is a successful renal replacement therapy; however, long-term PD leads to structural and functional peritoneal damage.The 8-OHdG level was significantly correlated with dialysate:plasma creatine ratio (r = 0.463, P < 0.05) and significantly inversely correlated with D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection) (r = -0.474, P < 0.05).The 8-OHdG level was also significantly correlated with MMP-2 level (r = 0.551, P < 0.05), but it was not correlated with the age of subjects, the duration of PD, or blood pressure.

View Article: PubMed Central - PubMed

Affiliation: Division of Nephrology, Department of Medicine, Jichi Medical University, Tochigi, Japan.

ABSTRACT

Purpose: Peritoneal dialysis (PD) is a successful renal replacement therapy; however, long-term PD leads to structural and functional peritoneal damage. Therefore, the monitoring and estimation of peritoneal function are important in PD patients. Oxidative stress has been implicated as one possible mechanism of peritoneal membrane damage. The aim of this study was to evaluate the association between an oxidative stress marker, 8-hydroxydeoxyguanosine (8-OHdG), and peritoneal damage in PD patients.

Methods: The authors evaluated 8-OHdG in drained dialysate by enzyme immunoassay to investigate the association between 8-OHdG and solute transport rate estimated by peritoneal equilibration test and matrix metalloproteinase-2 (MMP-2) level in 45 samples from 28 PD patients.

Results: The 8-OHdG level was significantly correlated with dialysate:plasma creatine ratio (r = 0.463, P < 0.05) and significantly inversely correlated with D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection) (r = -0.474, P < 0.05). The 8-OHdG level was also significantly correlated with MMP-2 level (r = 0.551, P < 0.05), but it was not correlated with the age of subjects, the duration of PD, or blood pressure.

Conclusion: The level of 8-OHdG in drained dialysate may be a useful novel marker of peritoneal damage in PD.

No MeSH data available.


Related in: MedlinePlus