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Phenotype characterization of embryoid body structures generated by a crystal comet effect tail in an intercellular cancer collision scenario.

Diaz JA, Murillo MF - Cancer Manag Res (2012)

Bottom Line: The structures are located in amniotic-like cavities and show characteristic somite-like embryologic segmentation.Immunophenotypic study has demonstrated exclusion factor positional identity in relation to enolase-immunopositive expression of embryoid body and human chorionic gonadotropin immunopositivity exclusion factor expression in the surrounding tissues.Reversal mechanisms in biology are intimately linked with DNA repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Hospital Departmental Villavicencio, Hospital Departmental Granada, Medicine School, University Cooperative of Colombia, Villavicencio, Meta, Colombia.

ABSTRACT
Cancer is, by definition, the uncontrolled growth of autonomous cells that eventually destroy adjacent tissues and generate architectural disorder. However, this concept cannot be totally true. In three well documented studies, we have demonstrated that cancer tissues produce order zones that evolve over time and generate embryoid body structures in a space-time interval. The authors decided to revise the macroscopic and microscopic material in well-developed malignant tumors in which embryoid bodies were identified to determine the phenotype characterization that serves as a guideline for easy recognition. The factors responsible for this morphogenesis are physical, bioelectric, and magnetic susceptibilities produced by crystals that act as molecular designers for the topographic gradients that guide the surrounding silhouette and establish tissue head-tail positional identities. The structures are located in amniotic-like cavities and show characteristic somite-like embryologic segmentation. Immunophenotypic study has demonstrated exclusion factor positional identity in relation to enolase-immunopositive expression of embryoid body and human chorionic gonadotropin immunopositivity exclusion factor expression in the surrounding tissues. The significance of these observations is that they can also be predicted by experimental image data collected by the Large Hadron Collider (LHC) accelerator at the European Organization for Nuclear Research, in which two-beam subatomic collision particles in the resulting debris show hyperorder domains similar to those identified by us in intercellular cancer collisions. Our findings suggest that we are dealing with true reverse biologic system information in an activated collective cancer stem cell memory, in which physics participates in the elaboration of geometric complexes and chiral biomolecules that serve to build bodies with embryoid print as it develops during gestation. Reversal mechanisms in biology are intimately linked with DNA repair. Further genotype studies must be carried out to determine whether the subproducts of these structures can be used in novel strategies to treat cancer.

No MeSH data available.


Related in: MedlinePlus

HCG immunopositivity localization related to embryoid body surrounding tissues and the walls of the triangular and hexagonal template platform where the structures are located. (A) H CG immunopositivity localization around inmmunonegativity embryoid body in a case of colon adenocarcinoma. (B) HCG-selective immunopositivity of the hexagonal template walls on which embryoid body is assembled in a case of undifferentiated sarcoma tumor. (C) HCG immunopositivity of surrounding tissue with inmmunonegativity of embryoid body structured inside hexagonal template platform in a case of renal cell carcinoma. (D) HCG selective spot walls immunopositivity of hexagonal geometric pattern in a case of papillary thyroid carcinoma. (E) Detachment subimage of (F) showing HCG selective immunopositivity of malignant giant cell in which two negative embryoid bodies are structured in their interior. Neighboring giant cell showing HCG immunonegativity, in a case of malignant fibrous histiocytoma. In Macroscopic fully developed malignant tumors is possible to identify spatial temporal organization that clearly reminds tissues fetus-placenta positional identities. (G) Detachment subimage of (H) a case of leiomyosarcoma. (I) Case of colon adenocarcinoma.Abbreviation: HCG, human chorionic gonadotropin.
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f8-cmar-4-009: HCG immunopositivity localization related to embryoid body surrounding tissues and the walls of the triangular and hexagonal template platform where the structures are located. (A) H CG immunopositivity localization around inmmunonegativity embryoid body in a case of colon adenocarcinoma. (B) HCG-selective immunopositivity of the hexagonal template walls on which embryoid body is assembled in a case of undifferentiated sarcoma tumor. (C) HCG immunopositivity of surrounding tissue with inmmunonegativity of embryoid body structured inside hexagonal template platform in a case of renal cell carcinoma. (D) HCG selective spot walls immunopositivity of hexagonal geometric pattern in a case of papillary thyroid carcinoma. (E) Detachment subimage of (F) showing HCG selective immunopositivity of malignant giant cell in which two negative embryoid bodies are structured in their interior. Neighboring giant cell showing HCG immunonegativity, in a case of malignant fibrous histiocytoma. In Macroscopic fully developed malignant tumors is possible to identify spatial temporal organization that clearly reminds tissues fetus-placenta positional identities. (G) Detachment subimage of (H) a case of leiomyosarcoma. (I) Case of colon adenocarcinoma.Abbreviation: HCG, human chorionic gonadotropin.

Mentions: Statistical analysis determined that 90 embryoid patterns had high immunopositivity for neuron-specific enolase in 67 cases (75%, χ2 = 17.16, P = 0.000034), which was negative in 23 cases (25%, Table 1).3 All 67 tissue-labeled sections were positive in relation to exclusion factor. Among the 67 tissue-labeled sections in which embryoid bodies showed immunopositivity exclusion factor in relation to enolase, 50 showed human chorionic gonadotropin immunopositivity and 17 were negative, indicating that, in labeled tissues, expression of enolase is limited to the structure and excludes the surrounding tissue. Similarly, human chorionic gonadotropin expression was negative with respect to the structure and positive for the surrounding tissue (Figure 7A–I). Localization of human chorionic gonadotropin immunopositivity is related to the tissues of the surrounding embryoid body and the walls of the triangular and hexagonal template platform where the structures are located (Figure 8A–F). According to the hypothesis, the variables are independent. Based on the chi-square test which yielded a chi value = 38.61, df = 1, P < 0.0001, and an alpha of 0.05, the hypothesis was rejected (Table 2).


Phenotype characterization of embryoid body structures generated by a crystal comet effect tail in an intercellular cancer collision scenario.

Diaz JA, Murillo MF - Cancer Manag Res (2012)

HCG immunopositivity localization related to embryoid body surrounding tissues and the walls of the triangular and hexagonal template platform where the structures are located. (A) H CG immunopositivity localization around inmmunonegativity embryoid body in a case of colon adenocarcinoma. (B) HCG-selective immunopositivity of the hexagonal template walls on which embryoid body is assembled in a case of undifferentiated sarcoma tumor. (C) HCG immunopositivity of surrounding tissue with inmmunonegativity of embryoid body structured inside hexagonal template platform in a case of renal cell carcinoma. (D) HCG selective spot walls immunopositivity of hexagonal geometric pattern in a case of papillary thyroid carcinoma. (E) Detachment subimage of (F) showing HCG selective immunopositivity of malignant giant cell in which two negative embryoid bodies are structured in their interior. Neighboring giant cell showing HCG immunonegativity, in a case of malignant fibrous histiocytoma. In Macroscopic fully developed malignant tumors is possible to identify spatial temporal organization that clearly reminds tissues fetus-placenta positional identities. (G) Detachment subimage of (H) a case of leiomyosarcoma. (I) Case of colon adenocarcinoma.Abbreviation: HCG, human chorionic gonadotropin.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3278205&req=5

f8-cmar-4-009: HCG immunopositivity localization related to embryoid body surrounding tissues and the walls of the triangular and hexagonal template platform where the structures are located. (A) H CG immunopositivity localization around inmmunonegativity embryoid body in a case of colon adenocarcinoma. (B) HCG-selective immunopositivity of the hexagonal template walls on which embryoid body is assembled in a case of undifferentiated sarcoma tumor. (C) HCG immunopositivity of surrounding tissue with inmmunonegativity of embryoid body structured inside hexagonal template platform in a case of renal cell carcinoma. (D) HCG selective spot walls immunopositivity of hexagonal geometric pattern in a case of papillary thyroid carcinoma. (E) Detachment subimage of (F) showing HCG selective immunopositivity of malignant giant cell in which two negative embryoid bodies are structured in their interior. Neighboring giant cell showing HCG immunonegativity, in a case of malignant fibrous histiocytoma. In Macroscopic fully developed malignant tumors is possible to identify spatial temporal organization that clearly reminds tissues fetus-placenta positional identities. (G) Detachment subimage of (H) a case of leiomyosarcoma. (I) Case of colon adenocarcinoma.Abbreviation: HCG, human chorionic gonadotropin.
Mentions: Statistical analysis determined that 90 embryoid patterns had high immunopositivity for neuron-specific enolase in 67 cases (75%, χ2 = 17.16, P = 0.000034), which was negative in 23 cases (25%, Table 1).3 All 67 tissue-labeled sections were positive in relation to exclusion factor. Among the 67 tissue-labeled sections in which embryoid bodies showed immunopositivity exclusion factor in relation to enolase, 50 showed human chorionic gonadotropin immunopositivity and 17 were negative, indicating that, in labeled tissues, expression of enolase is limited to the structure and excludes the surrounding tissue. Similarly, human chorionic gonadotropin expression was negative with respect to the structure and positive for the surrounding tissue (Figure 7A–I). Localization of human chorionic gonadotropin immunopositivity is related to the tissues of the surrounding embryoid body and the walls of the triangular and hexagonal template platform where the structures are located (Figure 8A–F). According to the hypothesis, the variables are independent. Based on the chi-square test which yielded a chi value = 38.61, df = 1, P < 0.0001, and an alpha of 0.05, the hypothesis was rejected (Table 2).

Bottom Line: The structures are located in amniotic-like cavities and show characteristic somite-like embryologic segmentation.Immunophenotypic study has demonstrated exclusion factor positional identity in relation to enolase-immunopositive expression of embryoid body and human chorionic gonadotropin immunopositivity exclusion factor expression in the surrounding tissues.Reversal mechanisms in biology are intimately linked with DNA repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Hospital Departmental Villavicencio, Hospital Departmental Granada, Medicine School, University Cooperative of Colombia, Villavicencio, Meta, Colombia.

ABSTRACT
Cancer is, by definition, the uncontrolled growth of autonomous cells that eventually destroy adjacent tissues and generate architectural disorder. However, this concept cannot be totally true. In three well documented studies, we have demonstrated that cancer tissues produce order zones that evolve over time and generate embryoid body structures in a space-time interval. The authors decided to revise the macroscopic and microscopic material in well-developed malignant tumors in which embryoid bodies were identified to determine the phenotype characterization that serves as a guideline for easy recognition. The factors responsible for this morphogenesis are physical, bioelectric, and magnetic susceptibilities produced by crystals that act as molecular designers for the topographic gradients that guide the surrounding silhouette and establish tissue head-tail positional identities. The structures are located in amniotic-like cavities and show characteristic somite-like embryologic segmentation. Immunophenotypic study has demonstrated exclusion factor positional identity in relation to enolase-immunopositive expression of embryoid body and human chorionic gonadotropin immunopositivity exclusion factor expression in the surrounding tissues. The significance of these observations is that they can also be predicted by experimental image data collected by the Large Hadron Collider (LHC) accelerator at the European Organization for Nuclear Research, in which two-beam subatomic collision particles in the resulting debris show hyperorder domains similar to those identified by us in intercellular cancer collisions. Our findings suggest that we are dealing with true reverse biologic system information in an activated collective cancer stem cell memory, in which physics participates in the elaboration of geometric complexes and chiral biomolecules that serve to build bodies with embryoid print as it develops during gestation. Reversal mechanisms in biology are intimately linked with DNA repair. Further genotype studies must be carried out to determine whether the subproducts of these structures can be used in novel strategies to treat cancer.

No MeSH data available.


Related in: MedlinePlus