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Phenotype characterization of embryoid body structures generated by a crystal comet effect tail in an intercellular cancer collision scenario.

Diaz JA, Murillo MF - Cancer Manag Res (2012)

Bottom Line: The structures are located in amniotic-like cavities and show characteristic somite-like embryologic segmentation.Immunophenotypic study has demonstrated exclusion factor positional identity in relation to enolase-immunopositive expression of embryoid body and human chorionic gonadotropin immunopositivity exclusion factor expression in the surrounding tissues.Reversal mechanisms in biology are intimately linked with DNA repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Hospital Departmental Villavicencio, Hospital Departmental Granada, Medicine School, University Cooperative of Colombia, Villavicencio, Meta, Colombia.

ABSTRACT
Cancer is, by definition, the uncontrolled growth of autonomous cells that eventually destroy adjacent tissues and generate architectural disorder. However, this concept cannot be totally true. In three well documented studies, we have demonstrated that cancer tissues produce order zones that evolve over time and generate embryoid body structures in a space-time interval. The authors decided to revise the macroscopic and microscopic material in well-developed malignant tumors in which embryoid bodies were identified to determine the phenotype characterization that serves as a guideline for easy recognition. The factors responsible for this morphogenesis are physical, bioelectric, and magnetic susceptibilities produced by crystals that act as molecular designers for the topographic gradients that guide the surrounding silhouette and establish tissue head-tail positional identities. The structures are located in amniotic-like cavities and show characteristic somite-like embryologic segmentation. Immunophenotypic study has demonstrated exclusion factor positional identity in relation to enolase-immunopositive expression of embryoid body and human chorionic gonadotropin immunopositivity exclusion factor expression in the surrounding tissues. The significance of these observations is that they can also be predicted by experimental image data collected by the Large Hadron Collider (LHC) accelerator at the European Organization for Nuclear Research, in which two-beam subatomic collision particles in the resulting debris show hyperorder domains similar to those identified by us in intercellular cancer collisions. Our findings suggest that we are dealing with true reverse biologic system information in an activated collective cancer stem cell memory, in which physics participates in the elaboration of geometric complexes and chiral biomolecules that serve to build bodies with embryoid print as it develops during gestation. Reversal mechanisms in biology are intimately linked with DNA repair. Further genotype studies must be carried out to determine whether the subproducts of these structures can be used in novel strategies to treat cancer.

No MeSH data available.


Related in: MedlinePlus

The genesis of embryoid bodies is intimately linked to displacement and migration of biological crystals derived from intercellular collision of tumor cell membranes. (A, B, C, and D) Migration of biological crystals in a case of lung adenocarcinoma, pleural fluid; case of cervical carcinoma in situ, vaginal smear; case of endometrial adenocarcinoma, endocervical smear and carcinoma of ovary and ascitic fluid, respectively. Papanicolaou staining (40×). (E and F) Embryoid bodies in a case of cervical carcinoma in situ, vaginal smear Papanicolaou staining (40×). (G) Crystal comet effect tail in a case of breast adenocarcinoma, fine needle aspiration, Papanicolaou staining (20×). (H) Detachment subimage of (I) image, which illustrates partial image taken from the projectile near the beginning of its trajectory as it falls through a tube filled with granular matter.Note: Reprinted figure with permission from supplemental video of Pacheco-Vázquez et al. Phys Rev Lett. 106, 218001 (2011). Copyright 2011 by the American Physical Society. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or part, without prior written permission from the American Physical Society. Available from: http://link.aps.org/doi/10.1103/PhysRevLett.106.218001.
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f1-cmar-4-009: The genesis of embryoid bodies is intimately linked to displacement and migration of biological crystals derived from intercellular collision of tumor cell membranes. (A, B, C, and D) Migration of biological crystals in a case of lung adenocarcinoma, pleural fluid; case of cervical carcinoma in situ, vaginal smear; case of endometrial adenocarcinoma, endocervical smear and carcinoma of ovary and ascitic fluid, respectively. Papanicolaou staining (40×). (E and F) Embryoid bodies in a case of cervical carcinoma in situ, vaginal smear Papanicolaou staining (40×). (G) Crystal comet effect tail in a case of breast adenocarcinoma, fine needle aspiration, Papanicolaou staining (20×). (H) Detachment subimage of (I) image, which illustrates partial image taken from the projectile near the beginning of its trajectory as it falls through a tube filled with granular matter.Note: Reprinted figure with permission from supplemental video of Pacheco-Vázquez et al. Phys Rev Lett. 106, 218001 (2011). Copyright 2011 by the American Physical Society. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or part, without prior written permission from the American Physical Society. Available from: http://link.aps.org/doi/10.1103/PhysRevLett.106.218001.

Mentions: By detailed morphologic observation of these structures, we were able to find and generalize a series of constant morphologic features in different types of tumors. Embryoid bodies appear in all types of malignant lesions. These structures are intimately linked to displacement and migration of biological crystals in the extracellular matrix derived from intercellular collision of tumor cell membranes, and generate crystalejected comet tail effects with strange helicity states that arise in the form of spin domains (Figure 1A and B).


Phenotype characterization of embryoid body structures generated by a crystal comet effect tail in an intercellular cancer collision scenario.

Diaz JA, Murillo MF - Cancer Manag Res (2012)

The genesis of embryoid bodies is intimately linked to displacement and migration of biological crystals derived from intercellular collision of tumor cell membranes. (A, B, C, and D) Migration of biological crystals in a case of lung adenocarcinoma, pleural fluid; case of cervical carcinoma in situ, vaginal smear; case of endometrial adenocarcinoma, endocervical smear and carcinoma of ovary and ascitic fluid, respectively. Papanicolaou staining (40×). (E and F) Embryoid bodies in a case of cervical carcinoma in situ, vaginal smear Papanicolaou staining (40×). (G) Crystal comet effect tail in a case of breast adenocarcinoma, fine needle aspiration, Papanicolaou staining (20×). (H) Detachment subimage of (I) image, which illustrates partial image taken from the projectile near the beginning of its trajectory as it falls through a tube filled with granular matter.Note: Reprinted figure with permission from supplemental video of Pacheco-Vázquez et al. Phys Rev Lett. 106, 218001 (2011). Copyright 2011 by the American Physical Society. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or part, without prior written permission from the American Physical Society. Available from: http://link.aps.org/doi/10.1103/PhysRevLett.106.218001.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3278205&req=5

f1-cmar-4-009: The genesis of embryoid bodies is intimately linked to displacement and migration of biological crystals derived from intercellular collision of tumor cell membranes. (A, B, C, and D) Migration of biological crystals in a case of lung adenocarcinoma, pleural fluid; case of cervical carcinoma in situ, vaginal smear; case of endometrial adenocarcinoma, endocervical smear and carcinoma of ovary and ascitic fluid, respectively. Papanicolaou staining (40×). (E and F) Embryoid bodies in a case of cervical carcinoma in situ, vaginal smear Papanicolaou staining (40×). (G) Crystal comet effect tail in a case of breast adenocarcinoma, fine needle aspiration, Papanicolaou staining (20×). (H) Detachment subimage of (I) image, which illustrates partial image taken from the projectile near the beginning of its trajectory as it falls through a tube filled with granular matter.Note: Reprinted figure with permission from supplemental video of Pacheco-Vázquez et al. Phys Rev Lett. 106, 218001 (2011). Copyright 2011 by the American Physical Society. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or part, without prior written permission from the American Physical Society. Available from: http://link.aps.org/doi/10.1103/PhysRevLett.106.218001.
Mentions: By detailed morphologic observation of these structures, we were able to find and generalize a series of constant morphologic features in different types of tumors. Embryoid bodies appear in all types of malignant lesions. These structures are intimately linked to displacement and migration of biological crystals in the extracellular matrix derived from intercellular collision of tumor cell membranes, and generate crystalejected comet tail effects with strange helicity states that arise in the form of spin domains (Figure 1A and B).

Bottom Line: The structures are located in amniotic-like cavities and show characteristic somite-like embryologic segmentation.Immunophenotypic study has demonstrated exclusion factor positional identity in relation to enolase-immunopositive expression of embryoid body and human chorionic gonadotropin immunopositivity exclusion factor expression in the surrounding tissues.Reversal mechanisms in biology are intimately linked with DNA repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Hospital Departmental Villavicencio, Hospital Departmental Granada, Medicine School, University Cooperative of Colombia, Villavicencio, Meta, Colombia.

ABSTRACT
Cancer is, by definition, the uncontrolled growth of autonomous cells that eventually destroy adjacent tissues and generate architectural disorder. However, this concept cannot be totally true. In three well documented studies, we have demonstrated that cancer tissues produce order zones that evolve over time and generate embryoid body structures in a space-time interval. The authors decided to revise the macroscopic and microscopic material in well-developed malignant tumors in which embryoid bodies were identified to determine the phenotype characterization that serves as a guideline for easy recognition. The factors responsible for this morphogenesis are physical, bioelectric, and magnetic susceptibilities produced by crystals that act as molecular designers for the topographic gradients that guide the surrounding silhouette and establish tissue head-tail positional identities. The structures are located in amniotic-like cavities and show characteristic somite-like embryologic segmentation. Immunophenotypic study has demonstrated exclusion factor positional identity in relation to enolase-immunopositive expression of embryoid body and human chorionic gonadotropin immunopositivity exclusion factor expression in the surrounding tissues. The significance of these observations is that they can also be predicted by experimental image data collected by the Large Hadron Collider (LHC) accelerator at the European Organization for Nuclear Research, in which two-beam subatomic collision particles in the resulting debris show hyperorder domains similar to those identified by us in intercellular cancer collisions. Our findings suggest that we are dealing with true reverse biologic system information in an activated collective cancer stem cell memory, in which physics participates in the elaboration of geometric complexes and chiral biomolecules that serve to build bodies with embryoid print as it develops during gestation. Reversal mechanisms in biology are intimately linked with DNA repair. Further genotype studies must be carried out to determine whether the subproducts of these structures can be used in novel strategies to treat cancer.

No MeSH data available.


Related in: MedlinePlus