Kinetochore-dependent microtubule rescue ensures their efficient and sustained interactions in early mitosis.
Bottom Line: Meanwhile, microtubule rescue distal to the kinetochore is also promoted by Stu2, which is transported by a kinesin-8 motor Kip3 along the microtubule from the kinetochore.Microtubule extension following rescue facilitates interaction with other widely scattered kinetochores, diminishing long delays in collecting the complete set of kinetochores by microtubules.Thus, kinetochore-dependent microtubule rescue ensures efficient and sustained kinetochore-microtubule interactions in early mitosis.
Affiliation: Wellcome Trust Centre for Gene Regulation & Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.Show MeSH
Related in: MedlinePlus
Mentions: Could Stu2 be a cargo that is transported by Kip3 along a MT? If so, we expect that Stu2 and Kip3 would move together from CEN3 along a MT. Colocalization of Stu2 and Kip3 signals was transiently observed at CEN3 (Figure 5Ai, 80 s). While Stu2 was transported from CEN3 along a MT toward its plus end (90–170 s), Stu2 signals continued to colocalize with a fraction of Kip3 signals. During 11 events of Stu2 transport from CEN3 toward the MT plus end, Stu2 signals colocalized continuously with Kip3 signals at most of the time points (20–130 s duration) (Figure 5Aii). In all 11 events of Stu2 transport, the relevant MTs showed rescue upon arrival of Stu2 at the MT plus end (e.g., Figure 5Ai, 170–180 s). While most Stu2 signals colocalized with a fraction of Kip3 signals during transport (Figure 5Ai, magenta arrowheads), other Kip3 signals showed movement along a MT without colocalization with Stu2 (white arrowheads).
Affiliation: Wellcome Trust Centre for Gene Regulation & Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.