Kinetochore-dependent microtubule rescue ensures their efficient and sustained interactions in early mitosis.
Bottom Line: Meanwhile, microtubule rescue distal to the kinetochore is also promoted by Stu2, which is transported by a kinesin-8 motor Kip3 along the microtubule from the kinetochore.Microtubule extension following rescue facilitates interaction with other widely scattered kinetochores, diminishing long delays in collecting the complete set of kinetochores by microtubules.Thus, kinetochore-dependent microtubule rescue ensures efficient and sustained kinetochore-microtubule interactions in early mitosis.
Affiliation: Wellcome Trust Centre for Gene Regulation & Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.Show MeSH
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Mentions: Lateral and end-on KT-MT attachments (see Introduction) found in physiological conditions (Kitamura et al., 2007) can be recapitulated in an engineered assay system, which allows more detailed observation of individual KT-MT interactions (Tanaka et al., 2005, 2007). We used this assay, in which KT assembly was delayed on a particular centromere (CEN3) by transcription from an adjacently inserted promoter (Figure 1A). This procedure increased the distance between CEN3 and the mitotic spindle. While cells were arrested in metaphase, we reactivated CEN3, leading to KT reassembly and interaction with the lateral surface of a single spindle-pole MT (Tanaka et al., 2005).
Affiliation: Wellcome Trust Centre for Gene Regulation & Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.