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Indoor air bacterial load and antibiotic susceptibility pattern of isolates in operating rooms and surgical wards at jimma university specialized hospital, southwest ethiopia.

Genet C, Kibru G, Tsegaye W - Ethiop J Health Sci (2011)

Bottom Line: Similarly the highest mean aerobic colony counts of 465cfu/hr and 461cfu/hr were observed in Female room-1 and room-2 respectively when compared to the acceptable range of 250-450cfu/hr.Higher degree of aerobic bacterial load was measured from operating rooms' and surgical wards' indoor air.Reducing foot trafficking, improving the ventilation system and routine cleaning has to be made to maintain the aerobic bacteria load with in optimal level.

View Article: PubMed Central - PubMed

Affiliation: School of Health Sciences, Samara University.

ABSTRACT

Background: Surgical site infection is the second most common health care associated infection. One of the risk factors for such infection is bacterial contamination of operating rooms' and surgical wards' indoor air. In view of that, the microbiological quality of air can be considered as a mirror of the hygienic condition of these rooms. Thus, the objective of this study was to determine the bacterial load and antibiotic susceptibility pattern of isolates in operating rooms' and surgical wards' indoor air of Jimma University Specialized Hospital.

Methods: A cross sectional study was conducted to measure indoor air microbial quality of operating rooms and surgical wards from October to January 2009/2010 on 108 indoor air samples collected in twelve rounds using purposive sampling technique by Settle Plate Method (Passive Air Sampling following 1/1/1 Schedule). Sample processing and antimicrobial susceptibility testing were done following standard bacteriological techniques. The data was analyzed using SPSS version 16 and interpreted according to scientifically determined baseline values initially suggested by Fisher.

Results: The mean aerobic colony counts obtained in OR-1(46cfu/hr) and OR-2(28cfu/hr) was far beyond the set 5-8cfu/hr acceptable standards for passive room. Similarly the highest mean aerobic colony counts of 465cfu/hr and 461cfu/hr were observed in Female room-1 and room-2 respectively when compared to the acceptable range of 250-450cfu/hr. In this study only 3 isolates of S. pyogenes and 48 isolates of S. aureus were identified. Over 66% of S. aureus was identified in Critical Zone of Operating rooms. All isolates of S. aureus showed 100% and 82.8% resistance to methicillin and ampicillin respectively.

Conclusion: Higher degree of aerobic bacterial load was measured from operating rooms' and surgical wards' indoor air. Reducing foot trafficking, improving the ventilation system and routine cleaning has to be made to maintain the aerobic bacteria load with in optimal level.

No MeSH data available.


Related in: MedlinePlus

Isolation rates of potential pathogenic bacteria from indoor air in SWs and ORs at JUSH; October–January 2009/2010: % of isolation (No. of isolates)FR-1: Female room-1, FR-2: Female room-2, OR-C: OR-critical zone, MR-1: Male room-1 MR-2 Male room-2, OR-I: OR-intermediate zone, OR-NC: OR-non critical zone
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Figure 2: Isolation rates of potential pathogenic bacteria from indoor air in SWs and ORs at JUSH; October–January 2009/2010: % of isolation (No. of isolates)FR-1: Female room-1, FR-2: Female room-2, OR-C: OR-critical zone, MR-1: Male room-1 MR-2 Male room-2, OR-I: OR-intermediate zone, OR-NC: OR-non critical zone

Mentions: Out of 108 air samples collected, only two bacterial species which are 3 isolates of Streptococcus pyogenes from SWs and 48 isolates of Staphylococcus aureus (70.8 % from SWs and 29.2 % from ORs) were identified. The isolation rate (66.7%) of S. aureus was significantly higher in Critical Zone than Intermediate and non Critical Zone of ORs (p<0.001). In addition, S. aureus was frequently isolated in FR-2 and MR-2 of SWs (Figure-2).


Indoor air bacterial load and antibiotic susceptibility pattern of isolates in operating rooms and surgical wards at jimma university specialized hospital, southwest ethiopia.

Genet C, Kibru G, Tsegaye W - Ethiop J Health Sci (2011)

Isolation rates of potential pathogenic bacteria from indoor air in SWs and ORs at JUSH; October–January 2009/2010: % of isolation (No. of isolates)FR-1: Female room-1, FR-2: Female room-2, OR-C: OR-critical zone, MR-1: Male room-1 MR-2 Male room-2, OR-I: OR-intermediate zone, OR-NC: OR-non critical zone
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3275854&req=5

Figure 2: Isolation rates of potential pathogenic bacteria from indoor air in SWs and ORs at JUSH; October–January 2009/2010: % of isolation (No. of isolates)FR-1: Female room-1, FR-2: Female room-2, OR-C: OR-critical zone, MR-1: Male room-1 MR-2 Male room-2, OR-I: OR-intermediate zone, OR-NC: OR-non critical zone
Mentions: Out of 108 air samples collected, only two bacterial species which are 3 isolates of Streptococcus pyogenes from SWs and 48 isolates of Staphylococcus aureus (70.8 % from SWs and 29.2 % from ORs) were identified. The isolation rate (66.7%) of S. aureus was significantly higher in Critical Zone than Intermediate and non Critical Zone of ORs (p<0.001). In addition, S. aureus was frequently isolated in FR-2 and MR-2 of SWs (Figure-2).

Bottom Line: Similarly the highest mean aerobic colony counts of 465cfu/hr and 461cfu/hr were observed in Female room-1 and room-2 respectively when compared to the acceptable range of 250-450cfu/hr.Higher degree of aerobic bacterial load was measured from operating rooms' and surgical wards' indoor air.Reducing foot trafficking, improving the ventilation system and routine cleaning has to be made to maintain the aerobic bacteria load with in optimal level.

View Article: PubMed Central - PubMed

Affiliation: School of Health Sciences, Samara University.

ABSTRACT

Background: Surgical site infection is the second most common health care associated infection. One of the risk factors for such infection is bacterial contamination of operating rooms' and surgical wards' indoor air. In view of that, the microbiological quality of air can be considered as a mirror of the hygienic condition of these rooms. Thus, the objective of this study was to determine the bacterial load and antibiotic susceptibility pattern of isolates in operating rooms' and surgical wards' indoor air of Jimma University Specialized Hospital.

Methods: A cross sectional study was conducted to measure indoor air microbial quality of operating rooms and surgical wards from October to January 2009/2010 on 108 indoor air samples collected in twelve rounds using purposive sampling technique by Settle Plate Method (Passive Air Sampling following 1/1/1 Schedule). Sample processing and antimicrobial susceptibility testing were done following standard bacteriological techniques. The data was analyzed using SPSS version 16 and interpreted according to scientifically determined baseline values initially suggested by Fisher.

Results: The mean aerobic colony counts obtained in OR-1(46cfu/hr) and OR-2(28cfu/hr) was far beyond the set 5-8cfu/hr acceptable standards for passive room. Similarly the highest mean aerobic colony counts of 465cfu/hr and 461cfu/hr were observed in Female room-1 and room-2 respectively when compared to the acceptable range of 250-450cfu/hr. In this study only 3 isolates of S. pyogenes and 48 isolates of S. aureus were identified. Over 66% of S. aureus was identified in Critical Zone of Operating rooms. All isolates of S. aureus showed 100% and 82.8% resistance to methicillin and ampicillin respectively.

Conclusion: Higher degree of aerobic bacterial load was measured from operating rooms' and surgical wards' indoor air. Reducing foot trafficking, improving the ventilation system and routine cleaning has to be made to maintain the aerobic bacteria load with in optimal level.

No MeSH data available.


Related in: MedlinePlus