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Fermentative production of isobutene.

van Leeuwen BN, van der Wulp AM, Duijnstee I, van Maris AJ, Straathof AJ - Appl. Microbiol. Biotechnol. (2012)

Bottom Line: The low aqueous solubility of isobutene might also minimize product toxicity to the microorganisms.The production costs estimate is 0.9 Euro kg(-1), which is reasonably competitive.About 70% of the production costs will be due to the costs of lignocellulose hydrolysate, which seems to be a preferred feedstock.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.

ABSTRACT
Isobutene (2-methylpropene) is one of those chemicals for which bio-based production might replace the petrochemical production in the future. Currently, more than 10 million metric tons of isobutene are produced on a yearly basis. Even though bio-based production might also be achieved through chemocatalytic or thermochemical methods, this review focuses on fermentative routes from sugars. Although biological isobutene formation is known since the 1970s, extensive metabolic engineering is required to achieve economically viable yields and productivities. Two recent metabolic engineering developments may enable anaerobic production close to the theoretical stoichiometry of 1isobutene + 2CO(2) + 2H(2)O per mol of glucose. One relies on the conversion of 3-hydroxyisovalerate to isobutene as a side activity of mevalonate diphosphate decarboxylase and the other on isobutanol dehydration as a side activity of engineered oleate hydratase. The latter resembles the fermentative production of isobutanol followed by isobutanol recovery and chemocatalytic dehydration. The advantage of a completely biological route is that not isobutanol, but instead gaseous isobutene is recovered from the fermenter together with CO(2). The low aqueous solubility of isobutene might also minimize product toxicity to the microorganisms. Although developments are at their infancy, the potential of a large scale fermentative isobutene production process is assessed. The production costs estimate is 0.9 Euro kg(-1), which is reasonably competitive. About 70% of the production costs will be due to the costs of lignocellulose hydrolysate, which seems to be a preferred feedstock.

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Industrial applications of isobutene
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getmorefigures.php?uid=PMC3275743&req=5

Fig1: Industrial applications of isobutene

Mentions: Nowadays, the need for renewable resources is constantly brought under discussion, providing interesting alternatives for sustainable production of chemicals and fuels, compared to the use of fossil feedstocks. With finite fossil feedstocks, it might be interesting to switch to fermentative processes in order to produce chemicals. Isobutene (2-methylpropene, see Fig. 1), which is currently produced at large scale by petrochemically cracking crude oil, could be one of those chemicals.Fig. 1


Fermentative production of isobutene.

van Leeuwen BN, van der Wulp AM, Duijnstee I, van Maris AJ, Straathof AJ - Appl. Microbiol. Biotechnol. (2012)

Industrial applications of isobutene
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3275743&req=5

Fig1: Industrial applications of isobutene
Mentions: Nowadays, the need for renewable resources is constantly brought under discussion, providing interesting alternatives for sustainable production of chemicals and fuels, compared to the use of fossil feedstocks. With finite fossil feedstocks, it might be interesting to switch to fermentative processes in order to produce chemicals. Isobutene (2-methylpropene, see Fig. 1), which is currently produced at large scale by petrochemically cracking crude oil, could be one of those chemicals.Fig. 1

Bottom Line: The low aqueous solubility of isobutene might also minimize product toxicity to the microorganisms.The production costs estimate is 0.9 Euro kg(-1), which is reasonably competitive.About 70% of the production costs will be due to the costs of lignocellulose hydrolysate, which seems to be a preferred feedstock.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.

ABSTRACT
Isobutene (2-methylpropene) is one of those chemicals for which bio-based production might replace the petrochemical production in the future. Currently, more than 10 million metric tons of isobutene are produced on a yearly basis. Even though bio-based production might also be achieved through chemocatalytic or thermochemical methods, this review focuses on fermentative routes from sugars. Although biological isobutene formation is known since the 1970s, extensive metabolic engineering is required to achieve economically viable yields and productivities. Two recent metabolic engineering developments may enable anaerobic production close to the theoretical stoichiometry of 1isobutene + 2CO(2) + 2H(2)O per mol of glucose. One relies on the conversion of 3-hydroxyisovalerate to isobutene as a side activity of mevalonate diphosphate decarboxylase and the other on isobutanol dehydration as a side activity of engineered oleate hydratase. The latter resembles the fermentative production of isobutanol followed by isobutanol recovery and chemocatalytic dehydration. The advantage of a completely biological route is that not isobutanol, but instead gaseous isobutene is recovered from the fermenter together with CO(2). The low aqueous solubility of isobutene might also minimize product toxicity to the microorganisms. Although developments are at their infancy, the potential of a large scale fermentative isobutene production process is assessed. The production costs estimate is 0.9 Euro kg(-1), which is reasonably competitive. About 70% of the production costs will be due to the costs of lignocellulose hydrolysate, which seems to be a preferred feedstock.

Show MeSH
Related in: MedlinePlus