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Apoptotic cell clearance in chronic inflammation of lateral neck cysts.

Dobros W, Burda K, Guzik K, Koziel J, Potempa J - Eur Arch Otorhinolaryngol (2011)

Bottom Line: Simultaneously, the expression of cytokines in macrophages exposed to cyst contents was measured.Macrophages co-cultured with cyst contents responded with variable expression of IL-6, TNF-α and IL-10.Together with local production of anti-inflammatory cytokines, this may fuel chronic inflammation in the cysts.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Regional Hospital, ul. Lwowska 178A, 33-100, Tarnow, Poland.

ABSTRACT
The mechanism driving accumulation of large numbers of apoptotic and necrotic neutrophils in inflamed lateral neck cysts (LNC), in the absence of infection, remains obscure. The cellular content of cysts obtained from 17 patients was co-cultured with human macrophages. Phagocytosis levels of cyst-derived neutrophils were determined and compared to the uptake of spontaneously apoptotic neutrophils. Simultaneously, the expression of cytokines in macrophages exposed to cyst contents was measured. In comparison to spontaneously apoptotic neutrophils, the phagocytosis of LNC-derived neutrophils by macrophages was inefficient. An inverse correlation between neutrophil content in LNC and their uptake was observed. Macrophages co-cultured with cyst contents responded with variable expression of IL-6, TNF-α and IL-10. The hindered clearance of apoptotic neutrophils in LNC may lead to secondary necrosis of these cells and stimulation of the inflammatory reaction. Together with local production of anti-inflammatory cytokines, this may fuel chronic inflammation in the cysts.

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A comparison of efficiency of phagocytosis of spontaneously apoptotic neutrophils and neutrophils derived from LNC content by human primary macrophages
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Fig1: A comparison of efficiency of phagocytosis of spontaneously apoptotic neutrophils and neutrophils derived from LNC content by human primary macrophages

Mentions: Based on previous findings [9] that LNC content is rich in apoptotic neutrophils, we designed experiments to test the susceptibility of these cells to engulfment by human macrophages. To this end, we first determined neutrophil elastase activity in all samples as a measure of neutrophil content; with significant activity found in 10 out of 17 samples. Next, using apoptotic neutrophils as a standard, we estimated that the cell content of our ten positive samples was in the range of 0.21 × 106–5.62 × 106 per mL of analyzed fluid (Table 2). To determine the phagocytosis of neutrophils in these ten samples by macrophages, the cellular content of cyst fluids were co-cultured with hMDMs, and the level of phagocytosis estimated as described above. In vitro prepared, spontaneously apoptotic neutrophils were taken up by hMDMs far more efficiently than the neutrophils present in LNC fluid. Interestingly, an inverse correlation between neutrophil content in LNC fluid samples was observed, since the uptake of neutrophils from samples with high content of these cells [sample 10, 11, 12, and 15, neutrophils content >2 × 106 per mL (Table 2)] was at very low levels (below 3%). In contrast, more than 10% of apoptotic neutrophils were phagocytosed by macrophages from four out of the six samples with a neutrophil content ranging from 0.21 × 106 to 0.86 × 106 per mL (Fig. 1). Regardless of these differences, it is clear that phagocytosis of neutrophils from cysts is very inefficient by comparison to clearance of apoptotic cells.Table 2


Apoptotic cell clearance in chronic inflammation of lateral neck cysts.

Dobros W, Burda K, Guzik K, Koziel J, Potempa J - Eur Arch Otorhinolaryngol (2011)

A comparison of efficiency of phagocytosis of spontaneously apoptotic neutrophils and neutrophils derived from LNC content by human primary macrophages
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3275742&req=5

Fig1: A comparison of efficiency of phagocytosis of spontaneously apoptotic neutrophils and neutrophils derived from LNC content by human primary macrophages
Mentions: Based on previous findings [9] that LNC content is rich in apoptotic neutrophils, we designed experiments to test the susceptibility of these cells to engulfment by human macrophages. To this end, we first determined neutrophil elastase activity in all samples as a measure of neutrophil content; with significant activity found in 10 out of 17 samples. Next, using apoptotic neutrophils as a standard, we estimated that the cell content of our ten positive samples was in the range of 0.21 × 106–5.62 × 106 per mL of analyzed fluid (Table 2). To determine the phagocytosis of neutrophils in these ten samples by macrophages, the cellular content of cyst fluids were co-cultured with hMDMs, and the level of phagocytosis estimated as described above. In vitro prepared, spontaneously apoptotic neutrophils were taken up by hMDMs far more efficiently than the neutrophils present in LNC fluid. Interestingly, an inverse correlation between neutrophil content in LNC fluid samples was observed, since the uptake of neutrophils from samples with high content of these cells [sample 10, 11, 12, and 15, neutrophils content >2 × 106 per mL (Table 2)] was at very low levels (below 3%). In contrast, more than 10% of apoptotic neutrophils were phagocytosed by macrophages from four out of the six samples with a neutrophil content ranging from 0.21 × 106 to 0.86 × 106 per mL (Fig. 1). Regardless of these differences, it is clear that phagocytosis of neutrophils from cysts is very inefficient by comparison to clearance of apoptotic cells.Table 2

Bottom Line: Simultaneously, the expression of cytokines in macrophages exposed to cyst contents was measured.Macrophages co-cultured with cyst contents responded with variable expression of IL-6, TNF-α and IL-10.Together with local production of anti-inflammatory cytokines, this may fuel chronic inflammation in the cysts.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Regional Hospital, ul. Lwowska 178A, 33-100, Tarnow, Poland.

ABSTRACT
The mechanism driving accumulation of large numbers of apoptotic and necrotic neutrophils in inflamed lateral neck cysts (LNC), in the absence of infection, remains obscure. The cellular content of cysts obtained from 17 patients was co-cultured with human macrophages. Phagocytosis levels of cyst-derived neutrophils were determined and compared to the uptake of spontaneously apoptotic neutrophils. Simultaneously, the expression of cytokines in macrophages exposed to cyst contents was measured. In comparison to spontaneously apoptotic neutrophils, the phagocytosis of LNC-derived neutrophils by macrophages was inefficient. An inverse correlation between neutrophil content in LNC and their uptake was observed. Macrophages co-cultured with cyst contents responded with variable expression of IL-6, TNF-α and IL-10. The hindered clearance of apoptotic neutrophils in LNC may lead to secondary necrosis of these cells and stimulation of the inflammatory reaction. Together with local production of anti-inflammatory cytokines, this may fuel chronic inflammation in the cysts.

Show MeSH
Related in: MedlinePlus