Limits...
Maturation-resistant dendritic cells ameliorate experimental autoimmune uveoretinitis.

Oh K, Kim YS, Lee DS - Immune Netw (2011)

Bottom Line: Dendritic cells were differentiated from bone marrow in the presence of recombinant GM-CSF.In this study, we used paraformaldehyde-fixed bone marrow-derived DCs to maintain them in an immature state.Pretreatment with fixed immature DCs, but not fixed mature DCs, ameliorated the disease progression of EAU by inhibiting uveitogenic CD4(+) T cell activation and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Immunology, Transplantation Research Institute, Seoul National University College of Medicine, Seoul 110-799, Korea.

ABSTRACT

Background: Endogenous uveitis is a chronic inflammatory eye disease of human, which frequently leads to blindness. Experimental autoimmune uveoretinitis (EAU) is an animal disease model of human endogenous uveitis and can be induced in susceptible animals by immunization with retinal antigens. EAU resembles the key immunological characteristics of human disease in that both are CD4(+) T-cell mediated diseases. Dendritic cells (DCs) are specialized antigen-presenting cells that are uniquely capable of activating naïve T cells. Regulation of immune responses through modulation of DCs has thus been tried extensively. Recently our group reported that donor strain-derived immature DC pretreatment successfully controlled the adverse immune response during allogeneic transplantation.

Methods: EAU was induced by immunization with human interphotoreceptor retinoid-binding protein (IRBP) peptide(1-20). Dendritic cells were differentiated from bone marrow in the presence of recombinant GM-CSF.

Results: In this study, we used paraformaldehyde-fixed bone marrow-derived DCs to maintain them in an immature state. Pretreatment with fixed immature DCs, but not fixed mature DCs, ameliorated the disease progression of EAU by inhibiting uveitogenic CD4(+) T cell activation and differentiation.

Conclusion: Application of iBMDC prepared according to the protocol of this study would provide an important treatment modality for the autoimmune diseases and transplantation rejection.

No MeSH data available.


Related in: MedlinePlus

Intracellular cytokine expression of uveitogenic CD4+ T cells. Draining lymph node cells were harvested from the mice received mBMDC, iBMDC, or media on the 7th day after immunization with IRBP peptide and were restimulated in vitro with PMA and ionomycin as described in Materials and Methods. Cells were analyzed using flowcytometer after staining with anti-CD4 and anti-IFN-γ antibodies. The data are representative of two independent determinations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3275710&req=5

Figure 3: Intracellular cytokine expression of uveitogenic CD4+ T cells. Draining lymph node cells were harvested from the mice received mBMDC, iBMDC, or media on the 7th day after immunization with IRBP peptide and were restimulated in vitro with PMA and ionomycin as described in Materials and Methods. Cells were analyzed using flowcytometer after staining with anti-CD4 and anti-IFN-γ antibodies. The data are representative of two independent determinations.

Mentions: Uveitogenic CD4+ T cell differentiation in the draining lymph nodes were also analyzed on the 7th day after immunization. IFN-γ-expressing CD4+ T cells among uveitogenic T cells were decreased in the iBMDC-pretreated mice and increased in the mBMDC-pretreated mice compared with media-pretreated control mice (Fig. 3).


Maturation-resistant dendritic cells ameliorate experimental autoimmune uveoretinitis.

Oh K, Kim YS, Lee DS - Immune Netw (2011)

Intracellular cytokine expression of uveitogenic CD4+ T cells. Draining lymph node cells were harvested from the mice received mBMDC, iBMDC, or media on the 7th day after immunization with IRBP peptide and were restimulated in vitro with PMA and ionomycin as described in Materials and Methods. Cells were analyzed using flowcytometer after staining with anti-CD4 and anti-IFN-γ antibodies. The data are representative of two independent determinations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3275710&req=5

Figure 3: Intracellular cytokine expression of uveitogenic CD4+ T cells. Draining lymph node cells were harvested from the mice received mBMDC, iBMDC, or media on the 7th day after immunization with IRBP peptide and were restimulated in vitro with PMA and ionomycin as described in Materials and Methods. Cells were analyzed using flowcytometer after staining with anti-CD4 and anti-IFN-γ antibodies. The data are representative of two independent determinations.
Mentions: Uveitogenic CD4+ T cell differentiation in the draining lymph nodes were also analyzed on the 7th day after immunization. IFN-γ-expressing CD4+ T cells among uveitogenic T cells were decreased in the iBMDC-pretreated mice and increased in the mBMDC-pretreated mice compared with media-pretreated control mice (Fig. 3).

Bottom Line: Dendritic cells were differentiated from bone marrow in the presence of recombinant GM-CSF.In this study, we used paraformaldehyde-fixed bone marrow-derived DCs to maintain them in an immature state.Pretreatment with fixed immature DCs, but not fixed mature DCs, ameliorated the disease progression of EAU by inhibiting uveitogenic CD4(+) T cell activation and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Immunology, Transplantation Research Institute, Seoul National University College of Medicine, Seoul 110-799, Korea.

ABSTRACT

Background: Endogenous uveitis is a chronic inflammatory eye disease of human, which frequently leads to blindness. Experimental autoimmune uveoretinitis (EAU) is an animal disease model of human endogenous uveitis and can be induced in susceptible animals by immunization with retinal antigens. EAU resembles the key immunological characteristics of human disease in that both are CD4(+) T-cell mediated diseases. Dendritic cells (DCs) are specialized antigen-presenting cells that are uniquely capable of activating naïve T cells. Regulation of immune responses through modulation of DCs has thus been tried extensively. Recently our group reported that donor strain-derived immature DC pretreatment successfully controlled the adverse immune response during allogeneic transplantation.

Methods: EAU was induced by immunization with human interphotoreceptor retinoid-binding protein (IRBP) peptide(1-20). Dendritic cells were differentiated from bone marrow in the presence of recombinant GM-CSF.

Results: In this study, we used paraformaldehyde-fixed bone marrow-derived DCs to maintain them in an immature state. Pretreatment with fixed immature DCs, but not fixed mature DCs, ameliorated the disease progression of EAU by inhibiting uveitogenic CD4(+) T cell activation and differentiation.

Conclusion: Application of iBMDC prepared according to the protocol of this study would provide an important treatment modality for the autoimmune diseases and transplantation rejection.

No MeSH data available.


Related in: MedlinePlus