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Dietary supplementation with probiotics improves hematopoiesis in malnourished mice.

Salva S, Merino MC, Agüero G, Gruppi A, Alvarez S - PLoS ONE (2012)

Bottom Line: This effect is associated with an increase in the number and functionality of immune cells, indicating that Lr could have some influence on myeloid and lymphoid cell production and maturation.Protein malnutrition significantly alters B cell development in BM.This work highlights the possibility of using immunobiotics to accelerate the recovery of lymphopoyesis in immunocompromised-malnourished hosts.

View Article: PubMed Central - PubMed

Affiliation: Reference Centre for Lactobacilli, CERELA-CONICET, Tucuman, Argentina.

ABSTRACT

Background: Lactobacillus rhamnosus CRL1505 (Lr) administered during the repletion of immunocompromised-malnourished mice improves the resistance against intestinal and respiratory infections. This effect is associated with an increase in the number and functionality of immune cells, indicating that Lr could have some influence on myeloid and lymphoid cell production and maturation.

Objective: This study analyzed the extent of the damage caused by malnutrition on myeloid and lymphoid cell development in the spleen and bone marrow (BM). We also evaluated the impact of immunobiotics on the recovery of hematopoiesis affected in malnourished mice.

Methods: Protein malnourished mice were fed on a balanced conventional diet for 7 or 14 consecutive d with or without supplemental Lr or fermented goat's milk (FGM). Malnourished mice and well-nourished mice were used as controls. Histological and flow cytometry studies were carried out in BM and spleen to study myeloid and lymphoid cells.

Results: Malnutrition induced quantitative alterations in spleen B and T cells; however, no alteration was observed in the ability of splenic B cells to produce immunoglobulins after challenge with LPS or CpG. The analysis of BM B cell subsets based on B220, CD24, IgM and IgD expression showed that malnutrition affected B cell development. In addition, BM myeloid cells decreased in malnourished mice. On the contrary, protein deprivation increased BM T cell number. These alterations were reverted with Lr or FGM repletion treatments since normal numbers of BM myeloid, T and B cells were observed in these groups.

Conclusions: Protein malnutrition significantly alters B cell development in BM. The treatment of malnourished mice with L. rhamnosus CRL1505 was able to induce a recovery of B cells that would explain its ability to increase immunity against infections. This work highlights the possibility of using immunobiotics to accelerate the recovery of lymphopoyesis in immunocompromised-malnourished hosts.

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A. Spleens photography obtained from well-nourished (WNC) and malnourished (MNC) mice.B. Total spleen cell number from WNC and MNC mice. The weight of the spleens of WNC and MNC was 178±5 mg and 84±6 mg respectively.
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pone-0031171-g001: A. Spleens photography obtained from well-nourished (WNC) and malnourished (MNC) mice.B. Total spleen cell number from WNC and MNC mice. The weight of the spleens of WNC and MNC was 178±5 mg and 84±6 mg respectively.

Mentions: Based on the fact that nutritional deprivation affects a specific antibody response [22]–[24], induces atrophy of lymphoid tissues and decreases the number of circulating T and B cells [7], [25], [26], we first studied splenic B cell number in our experimental model. As previously reported [7], [25], [26], we observed that a protein-free diet for 21 days significantly reduced the size of the spleen and the number of total splenic cells (Figure 1A, B). Additionally, the absolute number of mature (B220+IgM+) and immature (B220lowCD24high) splenic B cells as well as T cell (CD3+CD4+ and CD3+CD4−) number were significantly decreased in MN control (MNC) mice in comparison to well-nourished control (WNC) mice (Table 1). Despite the strong reduction in the total B cell number, the proportion of B cells in the spleen of MNC and WNC mice was similar (28,22±0,49% and 26,40±3,67%, respectively). Accordingly, IgM and IgG production by splenic B cells from MNC and WNC mice stimulated with LPS or CpG was similar, suggesting that protein deprivation affects B cell number but not their ability to produce Igs (Figure 2A, B).


Dietary supplementation with probiotics improves hematopoiesis in malnourished mice.

Salva S, Merino MC, Agüero G, Gruppi A, Alvarez S - PLoS ONE (2012)

A. Spleens photography obtained from well-nourished (WNC) and malnourished (MNC) mice.B. Total spleen cell number from WNC and MNC mice. The weight of the spleens of WNC and MNC was 178±5 mg and 84±6 mg respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3275617&req=5

pone-0031171-g001: A. Spleens photography obtained from well-nourished (WNC) and malnourished (MNC) mice.B. Total spleen cell number from WNC and MNC mice. The weight of the spleens of WNC and MNC was 178±5 mg and 84±6 mg respectively.
Mentions: Based on the fact that nutritional deprivation affects a specific antibody response [22]–[24], induces atrophy of lymphoid tissues and decreases the number of circulating T and B cells [7], [25], [26], we first studied splenic B cell number in our experimental model. As previously reported [7], [25], [26], we observed that a protein-free diet for 21 days significantly reduced the size of the spleen and the number of total splenic cells (Figure 1A, B). Additionally, the absolute number of mature (B220+IgM+) and immature (B220lowCD24high) splenic B cells as well as T cell (CD3+CD4+ and CD3+CD4−) number were significantly decreased in MN control (MNC) mice in comparison to well-nourished control (WNC) mice (Table 1). Despite the strong reduction in the total B cell number, the proportion of B cells in the spleen of MNC and WNC mice was similar (28,22±0,49% and 26,40±3,67%, respectively). Accordingly, IgM and IgG production by splenic B cells from MNC and WNC mice stimulated with LPS or CpG was similar, suggesting that protein deprivation affects B cell number but not their ability to produce Igs (Figure 2A, B).

Bottom Line: This effect is associated with an increase in the number and functionality of immune cells, indicating that Lr could have some influence on myeloid and lymphoid cell production and maturation.Protein malnutrition significantly alters B cell development in BM.This work highlights the possibility of using immunobiotics to accelerate the recovery of lymphopoyesis in immunocompromised-malnourished hosts.

View Article: PubMed Central - PubMed

Affiliation: Reference Centre for Lactobacilli, CERELA-CONICET, Tucuman, Argentina.

ABSTRACT

Background: Lactobacillus rhamnosus CRL1505 (Lr) administered during the repletion of immunocompromised-malnourished mice improves the resistance against intestinal and respiratory infections. This effect is associated with an increase in the number and functionality of immune cells, indicating that Lr could have some influence on myeloid and lymphoid cell production and maturation.

Objective: This study analyzed the extent of the damage caused by malnutrition on myeloid and lymphoid cell development in the spleen and bone marrow (BM). We also evaluated the impact of immunobiotics on the recovery of hematopoiesis affected in malnourished mice.

Methods: Protein malnourished mice were fed on a balanced conventional diet for 7 or 14 consecutive d with or without supplemental Lr or fermented goat's milk (FGM). Malnourished mice and well-nourished mice were used as controls. Histological and flow cytometry studies were carried out in BM and spleen to study myeloid and lymphoid cells.

Results: Malnutrition induced quantitative alterations in spleen B and T cells; however, no alteration was observed in the ability of splenic B cells to produce immunoglobulins after challenge with LPS or CpG. The analysis of BM B cell subsets based on B220, CD24, IgM and IgD expression showed that malnutrition affected B cell development. In addition, BM myeloid cells decreased in malnourished mice. On the contrary, protein deprivation increased BM T cell number. These alterations were reverted with Lr or FGM repletion treatments since normal numbers of BM myeloid, T and B cells were observed in these groups.

Conclusions: Protein malnutrition significantly alters B cell development in BM. The treatment of malnourished mice with L. rhamnosus CRL1505 was able to induce a recovery of B cells that would explain its ability to increase immunity against infections. This work highlights the possibility of using immunobiotics to accelerate the recovery of lymphopoyesis in immunocompromised-malnourished hosts.

Show MeSH
Related in: MedlinePlus