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Effects of soil water and nitrogen on growth and photosynthetic response of Manchurian ash (Fraxinus mandshurica) seedlings in northeastern China.

Wang M, Shi S, Lin F, Hao Z, Jiang P, Dai G - PLoS ONE (2012)

Bottom Line: LW treatment significantly decreased biomass production and leaf N content, but they showed marked increases in N addition.The positive relationships were found between photosynthetic capacity, leaf N content, and SLA in response to water and N supply in the seedling.Our study has presented better understanding of the interactions between soil water and N on the growth and photosynthetic response in F. mandschurica seedlings, which may provide novel insights on the potential responses of the forest ecosystem to climate change associated with increasing N deposition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China. wangmiao@iae.ac.cn

ABSTRACT

Background: Soil water and nitrogen (N) are considered to be the main environmental factors limiting plant growth and photosynthetic capacity. However, less is known about the interactive effects of soil water and N on tree growth and photosynthetic response in the temperate ecosystem.

Methods/principal findings: We applied N and water, alone and in combination, and investigated the combined effect of different water and N regimes on growth and photosynthetic responses of Fraxinus mandshurica seedlings. The seedlings were exposed to three water regimes including natural precipitation (CK), higher precipitation (HW) (CK +30%) and lower precipitation (LW) (CK -30%), and both with and without N addition for two growing seasons. We demonstrated that water and N supply led to a significant increase in the growth and biomass production of the seedlings. LW treatment significantly decreased biomass production and leaf N content, but they showed marked increases in N addition. N addition could enhance the photosynthetic capability under HW and CK conditions. Leaf chlorophyll content and the initial activity of Rubisco were dramatically increased by N addition regardless of soil water condition. The positive relationships were found between photosynthetic capacity, leaf N content, and SLA in response to water and N supply in the seedling. Rubisco expression was up-regulated by N addition with decreasing soil water content. Immunofluorescent staining showed that the labeling for Rubisco was relatively low in leaves of the seedlings under LW condition. The accumulation of Rubisco was increased in leaf tissues of LW by N addition.

Conclusions/significance: Our study has presented better understanding of the interactions between soil water and N on the growth and photosynthetic response in F. mandschurica seedlings, which may provide novel insights on the potential responses of the forest ecosystem to climate change associated with increasing N deposition.

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Confocal microscopy to show in situ immunolocalization of Rubisco in leaves of F. mandshurica seedlings.Label appears as green particles and nuclei are stained with DAPI (blue). (A) HW; (B) CK; (C) LW; (D) +NHW; (E) +NCK; (F) +NLW.
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pone-0030754-g008: Confocal microscopy to show in situ immunolocalization of Rubisco in leaves of F. mandshurica seedlings.Label appears as green particles and nuclei are stained with DAPI (blue). (A) HW; (B) CK; (C) LW; (D) +NHW; (E) +NCK; (F) +NLW.

Mentions: We further detected the distribution of Rubisco in the leaves of F. mandshurica seedlings. Immunofluorescent staining showed that Rubisco was found in chloroplasts throughout the leaf chlorenchyma in the seedlings (Fig. 8). Labeling for Rubisco was abundant in chloroplasts of leaf tissues of HW and CK, while the labeling was relatively low in leaves of the seedlings under LW condition. We observed that labeling for Rubisco was also concentrated in the chloroplasts of leaf chlorenchyma after N addition. The accumulation of Rubisco has increased in leaf tissues of LW by N addition, which was similar to the result from the expression of Rubisco by Western blots.


Effects of soil water and nitrogen on growth and photosynthetic response of Manchurian ash (Fraxinus mandshurica) seedlings in northeastern China.

Wang M, Shi S, Lin F, Hao Z, Jiang P, Dai G - PLoS ONE (2012)

Confocal microscopy to show in situ immunolocalization of Rubisco in leaves of F. mandshurica seedlings.Label appears as green particles and nuclei are stained with DAPI (blue). (A) HW; (B) CK; (C) LW; (D) +NHW; (E) +NCK; (F) +NLW.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3275608&req=5

pone-0030754-g008: Confocal microscopy to show in situ immunolocalization of Rubisco in leaves of F. mandshurica seedlings.Label appears as green particles and nuclei are stained with DAPI (blue). (A) HW; (B) CK; (C) LW; (D) +NHW; (E) +NCK; (F) +NLW.
Mentions: We further detected the distribution of Rubisco in the leaves of F. mandshurica seedlings. Immunofluorescent staining showed that Rubisco was found in chloroplasts throughout the leaf chlorenchyma in the seedlings (Fig. 8). Labeling for Rubisco was abundant in chloroplasts of leaf tissues of HW and CK, while the labeling was relatively low in leaves of the seedlings under LW condition. We observed that labeling for Rubisco was also concentrated in the chloroplasts of leaf chlorenchyma after N addition. The accumulation of Rubisco has increased in leaf tissues of LW by N addition, which was similar to the result from the expression of Rubisco by Western blots.

Bottom Line: LW treatment significantly decreased biomass production and leaf N content, but they showed marked increases in N addition.The positive relationships were found between photosynthetic capacity, leaf N content, and SLA in response to water and N supply in the seedling.Our study has presented better understanding of the interactions between soil water and N on the growth and photosynthetic response in F. mandschurica seedlings, which may provide novel insights on the potential responses of the forest ecosystem to climate change associated with increasing N deposition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China. wangmiao@iae.ac.cn

ABSTRACT

Background: Soil water and nitrogen (N) are considered to be the main environmental factors limiting plant growth and photosynthetic capacity. However, less is known about the interactive effects of soil water and N on tree growth and photosynthetic response in the temperate ecosystem.

Methods/principal findings: We applied N and water, alone and in combination, and investigated the combined effect of different water and N regimes on growth and photosynthetic responses of Fraxinus mandshurica seedlings. The seedlings were exposed to three water regimes including natural precipitation (CK), higher precipitation (HW) (CK +30%) and lower precipitation (LW) (CK -30%), and both with and without N addition for two growing seasons. We demonstrated that water and N supply led to a significant increase in the growth and biomass production of the seedlings. LW treatment significantly decreased biomass production and leaf N content, but they showed marked increases in N addition. N addition could enhance the photosynthetic capability under HW and CK conditions. Leaf chlorophyll content and the initial activity of Rubisco were dramatically increased by N addition regardless of soil water condition. The positive relationships were found between photosynthetic capacity, leaf N content, and SLA in response to water and N supply in the seedling. Rubisco expression was up-regulated by N addition with decreasing soil water content. Immunofluorescent staining showed that the labeling for Rubisco was relatively low in leaves of the seedlings under LW condition. The accumulation of Rubisco was increased in leaf tissues of LW by N addition.

Conclusions/significance: Our study has presented better understanding of the interactions between soil water and N on the growth and photosynthetic response in F. mandschurica seedlings, which may provide novel insights on the potential responses of the forest ecosystem to climate change associated with increasing N deposition.

Show MeSH
Related in: MedlinePlus