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Effects of soil water and nitrogen on growth and photosynthetic response of Manchurian ash (Fraxinus mandshurica) seedlings in northeastern China.

Wang M, Shi S, Lin F, Hao Z, Jiang P, Dai G - PLoS ONE (2012)

Bottom Line: LW treatment significantly decreased biomass production and leaf N content, but they showed marked increases in N addition.The positive relationships were found between photosynthetic capacity, leaf N content, and SLA in response to water and N supply in the seedling.Our study has presented better understanding of the interactions between soil water and N on the growth and photosynthetic response in F. mandschurica seedlings, which may provide novel insights on the potential responses of the forest ecosystem to climate change associated with increasing N deposition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China. wangmiao@iae.ac.cn

ABSTRACT

Background: Soil water and nitrogen (N) are considered to be the main environmental factors limiting plant growth and photosynthetic capacity. However, less is known about the interactive effects of soil water and N on tree growth and photosynthetic response in the temperate ecosystem.

Methods/principal findings: We applied N and water, alone and in combination, and investigated the combined effect of different water and N regimes on growth and photosynthetic responses of Fraxinus mandshurica seedlings. The seedlings were exposed to three water regimes including natural precipitation (CK), higher precipitation (HW) (CK +30%) and lower precipitation (LW) (CK -30%), and both with and without N addition for two growing seasons. We demonstrated that water and N supply led to a significant increase in the growth and biomass production of the seedlings. LW treatment significantly decreased biomass production and leaf N content, but they showed marked increases in N addition. N addition could enhance the photosynthetic capability under HW and CK conditions. Leaf chlorophyll content and the initial activity of Rubisco were dramatically increased by N addition regardless of soil water condition. The positive relationships were found between photosynthetic capacity, leaf N content, and SLA in response to water and N supply in the seedling. Rubisco expression was up-regulated by N addition with decreasing soil water content. Immunofluorescent staining showed that the labeling for Rubisco was relatively low in leaves of the seedlings under LW condition. The accumulation of Rubisco was increased in leaf tissues of LW by N addition.

Conclusions/significance: Our study has presented better understanding of the interactions between soil water and N on the growth and photosynthetic response in F. mandschurica seedlings, which may provide novel insights on the potential responses of the forest ecosystem to climate change associated with increasing N deposition.

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Related in: MedlinePlus

Western blot analysis of large subunits of Rubisco in leaves of F. mandshurica seedlings (panel A).The relative expression level is shown as the ratio of the band intensities between different treatments and CK with the analysis by Quantity One software (panel B).
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pone-0030754-g007: Western blot analysis of large subunits of Rubisco in leaves of F. mandshurica seedlings (panel A).The relative expression level is shown as the ratio of the band intensities between different treatments and CK with the analysis by Quantity One software (panel B).

Mentions: In addition to the activity and activation state of Rubisco, soil N and water might affect photosynthetic responses by regulating expression of Rubisco in the leaves of the seedlings. Therefore, we further analyzed the effects of N addition and water regimes on the expression of the large subunits in Rubisco in the leaves of F. mandshurica seedlings. The leaf protein of F. mandshurica seedlings was separated by means of SDS-PAGE, and a 55 kDa distinct band showed up by western blot analysis (Fig. 7A). The analysis of band intensity indicated that the expression of Rubisco was down-regulated by LW treatment, while the expression of the protein wasn't significantly influenced in HW treatment (Fig. 7B). The expression level of Rubisco was higher in N addition and LW treatment than that in LW condition, suggesting that N addition increased the protein expression of Rubisco under LW condition. However, under HW and CK conditions, N addition didn't induce a change in the expression of.


Effects of soil water and nitrogen on growth and photosynthetic response of Manchurian ash (Fraxinus mandshurica) seedlings in northeastern China.

Wang M, Shi S, Lin F, Hao Z, Jiang P, Dai G - PLoS ONE (2012)

Western blot analysis of large subunits of Rubisco in leaves of F. mandshurica seedlings (panel A).The relative expression level is shown as the ratio of the band intensities between different treatments and CK with the analysis by Quantity One software (panel B).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3275608&req=5

pone-0030754-g007: Western blot analysis of large subunits of Rubisco in leaves of F. mandshurica seedlings (panel A).The relative expression level is shown as the ratio of the band intensities between different treatments and CK with the analysis by Quantity One software (panel B).
Mentions: In addition to the activity and activation state of Rubisco, soil N and water might affect photosynthetic responses by regulating expression of Rubisco in the leaves of the seedlings. Therefore, we further analyzed the effects of N addition and water regimes on the expression of the large subunits in Rubisco in the leaves of F. mandshurica seedlings. The leaf protein of F. mandshurica seedlings was separated by means of SDS-PAGE, and a 55 kDa distinct band showed up by western blot analysis (Fig. 7A). The analysis of band intensity indicated that the expression of Rubisco was down-regulated by LW treatment, while the expression of the protein wasn't significantly influenced in HW treatment (Fig. 7B). The expression level of Rubisco was higher in N addition and LW treatment than that in LW condition, suggesting that N addition increased the protein expression of Rubisco under LW condition. However, under HW and CK conditions, N addition didn't induce a change in the expression of.

Bottom Line: LW treatment significantly decreased biomass production and leaf N content, but they showed marked increases in N addition.The positive relationships were found between photosynthetic capacity, leaf N content, and SLA in response to water and N supply in the seedling.Our study has presented better understanding of the interactions between soil water and N on the growth and photosynthetic response in F. mandschurica seedlings, which may provide novel insights on the potential responses of the forest ecosystem to climate change associated with increasing N deposition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China. wangmiao@iae.ac.cn

ABSTRACT

Background: Soil water and nitrogen (N) are considered to be the main environmental factors limiting plant growth and photosynthetic capacity. However, less is known about the interactive effects of soil water and N on tree growth and photosynthetic response in the temperate ecosystem.

Methods/principal findings: We applied N and water, alone and in combination, and investigated the combined effect of different water and N regimes on growth and photosynthetic responses of Fraxinus mandshurica seedlings. The seedlings were exposed to three water regimes including natural precipitation (CK), higher precipitation (HW) (CK +30%) and lower precipitation (LW) (CK -30%), and both with and without N addition for two growing seasons. We demonstrated that water and N supply led to a significant increase in the growth and biomass production of the seedlings. LW treatment significantly decreased biomass production and leaf N content, but they showed marked increases in N addition. N addition could enhance the photosynthetic capability under HW and CK conditions. Leaf chlorophyll content and the initial activity of Rubisco were dramatically increased by N addition regardless of soil water condition. The positive relationships were found between photosynthetic capacity, leaf N content, and SLA in response to water and N supply in the seedling. Rubisco expression was up-regulated by N addition with decreasing soil water content. Immunofluorescent staining showed that the labeling for Rubisco was relatively low in leaves of the seedlings under LW condition. The accumulation of Rubisco was increased in leaf tissues of LW by N addition.

Conclusions/significance: Our study has presented better understanding of the interactions between soil water and N on the growth and photosynthetic response in F. mandschurica seedlings, which may provide novel insights on the potential responses of the forest ecosystem to climate change associated with increasing N deposition.

Show MeSH
Related in: MedlinePlus