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Correlation of group C meningococcal conjugate vaccine response with B- and T-lymphocyte activity.

Wing JB, Smart L, Borrow R, Findlow J, Findlow H, Lees A, Foster RA, Carlring J, Read RC, Heath AW - PLoS ONE (2012)

Bottom Line: Despite the success of conjugate vaccination against meningococcal group C (MenC) disease, post-vaccination, some individuals still exhibit rapid waning of initially protective bactericidal antibody levels.The mechanism of this relative loss of humoral protection remains undetermined.We have found that healthy volunteers who lose protective MenC antibody levels one year after receipt of glycoconjugate vaccine exhibit no detectable cellular defect in polyclonal B- or T-cell activation, proliferation or the B-memory pool.

View Article: PubMed Central - PubMed

Affiliation: Department of Infection and Immunity, University of Sheffield Medical School, Sheffield, United Kingdom.

ABSTRACT
Despite the success of conjugate vaccination against meningococcal group C (MenC) disease, post-vaccination, some individuals still exhibit rapid waning of initially protective bactericidal antibody levels. The mechanism of this relative loss of humoral protection remains undetermined. In this report we have investigated the relationship between T- and B-cell activation and co-stimulation and the loss of protective antibody titers. We have found that healthy volunteers who lose protective MenC antibody levels one year after receipt of glycoconjugate vaccine exhibit no detectable cellular defect in polyclonal B- or T-cell activation, proliferation or the B-memory pool. This suggests that the processes underlying the more rapid loss of antibody levels are independent of defects in either initial T- or B-cell activation.

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Related in: MedlinePlus

T-cell and B-cell activation/proliferation in response to polyclonal stimuli.PBMCs were extracted from subjects with a MenC SBA titer <8 and from matched controls one year post vaccination and stimulated for 96 hours with plate bound αCD3±αCD28 and/or the TI-II antigen mimic, α-δ-dex. Activation and proliferation was measured by expression of A) CD25 Median Fluorescence Intensity (MFI) of CD4+ cells, B) CD27 MFI of CD4+ cells, C) Proliferation of CD4+ cells, D) CD25 MFI CD19+ cells, E) CD86 MFI of CD19+ cells and F) Proliferation of CD19+ cells. n = 11, data square rooted for normality, One-way ANOVA+ Bonferroni selected pairs post-test (SBA low vs. SBA high).
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pone-0031160-g002: T-cell and B-cell activation/proliferation in response to polyclonal stimuli.PBMCs were extracted from subjects with a MenC SBA titer <8 and from matched controls one year post vaccination and stimulated for 96 hours with plate bound αCD3±αCD28 and/or the TI-II antigen mimic, α-δ-dex. Activation and proliferation was measured by expression of A) CD25 Median Fluorescence Intensity (MFI) of CD4+ cells, B) CD27 MFI of CD4+ cells, C) Proliferation of CD4+ cells, D) CD25 MFI CD19+ cells, E) CD86 MFI of CD19+ cells and F) Proliferation of CD19+ cells. n = 11, data square rooted for normality, One-way ANOVA+ Bonferroni selected pairs post-test (SBA low vs. SBA high).

Mentions: To investigate the possibility that poor retention of anti-MenC SBA concentrations may be related to an observable defect in cellular activation of B- and T-cells we compared the response to polyclonal stimulation of SBA low donors (at 12 months) to age and sex matched controls with protective SBA titers. There was a trend towards increased T-cell activation/proliferation with concurrent lower B-cell activation seen in the SBA low donors in comparison to the SBA high donors but this was not statistically significant (Figure 2). In addition to analysis of the total B-cell population we also examined any differences in the CD27+ memory population alone, again we did not see any significant differences between the SBA low and high groups, in B-cell proliferation or activation marker expression (Figure S1).


Correlation of group C meningococcal conjugate vaccine response with B- and T-lymphocyte activity.

Wing JB, Smart L, Borrow R, Findlow J, Findlow H, Lees A, Foster RA, Carlring J, Read RC, Heath AW - PLoS ONE (2012)

T-cell and B-cell activation/proliferation in response to polyclonal stimuli.PBMCs were extracted from subjects with a MenC SBA titer <8 and from matched controls one year post vaccination and stimulated for 96 hours with plate bound αCD3±αCD28 and/or the TI-II antigen mimic, α-δ-dex. Activation and proliferation was measured by expression of A) CD25 Median Fluorescence Intensity (MFI) of CD4+ cells, B) CD27 MFI of CD4+ cells, C) Proliferation of CD4+ cells, D) CD25 MFI CD19+ cells, E) CD86 MFI of CD19+ cells and F) Proliferation of CD19+ cells. n = 11, data square rooted for normality, One-way ANOVA+ Bonferroni selected pairs post-test (SBA low vs. SBA high).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3275607&req=5

pone-0031160-g002: T-cell and B-cell activation/proliferation in response to polyclonal stimuli.PBMCs were extracted from subjects with a MenC SBA titer <8 and from matched controls one year post vaccination and stimulated for 96 hours with plate bound αCD3±αCD28 and/or the TI-II antigen mimic, α-δ-dex. Activation and proliferation was measured by expression of A) CD25 Median Fluorescence Intensity (MFI) of CD4+ cells, B) CD27 MFI of CD4+ cells, C) Proliferation of CD4+ cells, D) CD25 MFI CD19+ cells, E) CD86 MFI of CD19+ cells and F) Proliferation of CD19+ cells. n = 11, data square rooted for normality, One-way ANOVA+ Bonferroni selected pairs post-test (SBA low vs. SBA high).
Mentions: To investigate the possibility that poor retention of anti-MenC SBA concentrations may be related to an observable defect in cellular activation of B- and T-cells we compared the response to polyclonal stimulation of SBA low donors (at 12 months) to age and sex matched controls with protective SBA titers. There was a trend towards increased T-cell activation/proliferation with concurrent lower B-cell activation seen in the SBA low donors in comparison to the SBA high donors but this was not statistically significant (Figure 2). In addition to analysis of the total B-cell population we also examined any differences in the CD27+ memory population alone, again we did not see any significant differences between the SBA low and high groups, in B-cell proliferation or activation marker expression (Figure S1).

Bottom Line: Despite the success of conjugate vaccination against meningococcal group C (MenC) disease, post-vaccination, some individuals still exhibit rapid waning of initially protective bactericidal antibody levels.The mechanism of this relative loss of humoral protection remains undetermined.We have found that healthy volunteers who lose protective MenC antibody levels one year after receipt of glycoconjugate vaccine exhibit no detectable cellular defect in polyclonal B- or T-cell activation, proliferation or the B-memory pool.

View Article: PubMed Central - PubMed

Affiliation: Department of Infection and Immunity, University of Sheffield Medical School, Sheffield, United Kingdom.

ABSTRACT
Despite the success of conjugate vaccination against meningococcal group C (MenC) disease, post-vaccination, some individuals still exhibit rapid waning of initially protective bactericidal antibody levels. The mechanism of this relative loss of humoral protection remains undetermined. In this report we have investigated the relationship between T- and B-cell activation and co-stimulation and the loss of protective antibody titers. We have found that healthy volunteers who lose protective MenC antibody levels one year after receipt of glycoconjugate vaccine exhibit no detectable cellular defect in polyclonal B- or T-cell activation, proliferation or the B-memory pool. This suggests that the processes underlying the more rapid loss of antibody levels are independent of defects in either initial T- or B-cell activation.

Show MeSH
Related in: MedlinePlus