Limits...
Correlation of group C meningococcal conjugate vaccine response with B- and T-lymphocyte activity.

Wing JB, Smart L, Borrow R, Findlow J, Findlow H, Lees A, Foster RA, Carlring J, Read RC, Heath AW - PLoS ONE (2012)

Bottom Line: Despite the success of conjugate vaccination against meningococcal group C (MenC) disease, post-vaccination, some individuals still exhibit rapid waning of initially protective bactericidal antibody levels.The mechanism of this relative loss of humoral protection remains undetermined.We have found that healthy volunteers who lose protective MenC antibody levels one year after receipt of glycoconjugate vaccine exhibit no detectable cellular defect in polyclonal B- or T-cell activation, proliferation or the B-memory pool.

View Article: PubMed Central - PubMed

Affiliation: Department of Infection and Immunity, University of Sheffield Medical School, Sheffield, United Kingdom.

ABSTRACT
Despite the success of conjugate vaccination against meningococcal group C (MenC) disease, post-vaccination, some individuals still exhibit rapid waning of initially protective bactericidal antibody levels. The mechanism of this relative loss of humoral protection remains undetermined. In this report we have investigated the relationship between T- and B-cell activation and co-stimulation and the loss of protective antibody titers. We have found that healthy volunteers who lose protective MenC antibody levels one year after receipt of glycoconjugate vaccine exhibit no detectable cellular defect in polyclonal B- or T-cell activation, proliferation or the B-memory pool. This suggests that the processes underlying the more rapid loss of antibody levels are independent of defects in either initial T- or B-cell activation.

Show MeSH

Related in: MedlinePlus

T-cell CD3 and CD28 expression and B-cell memory pool distribution.PBMCs were extracted from subjects with a MenC SBA titer <8 and matched controls one year post vaccination and levels of T-cell CD3 and CD28 expression B-cell memory subsets were examined by flowcytometry. n = 11, One-way ANOVA+ Bonferroni selected pairs post-test (SBA low vs. SBA high).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3275607&req=5

pone-0031160-g001: T-cell CD3 and CD28 expression and B-cell memory pool distribution.PBMCs were extracted from subjects with a MenC SBA titer <8 and matched controls one year post vaccination and levels of T-cell CD3 and CD28 expression B-cell memory subsets were examined by flowcytometry. n = 11, One-way ANOVA+ Bonferroni selected pairs post-test (SBA low vs. SBA high).

Mentions: To determine if any observed differences in anti-CD3 and anti-CD28 response were due to differences in CD3 and CD28 expression by CD4+ cells we analysed the level of surface expression of these markers. No significant difference was seen between the SBA low group (SBA titer <8) and the matched controls (Figure 1a).


Correlation of group C meningococcal conjugate vaccine response with B- and T-lymphocyte activity.

Wing JB, Smart L, Borrow R, Findlow J, Findlow H, Lees A, Foster RA, Carlring J, Read RC, Heath AW - PLoS ONE (2012)

T-cell CD3 and CD28 expression and B-cell memory pool distribution.PBMCs were extracted from subjects with a MenC SBA titer <8 and matched controls one year post vaccination and levels of T-cell CD3 and CD28 expression B-cell memory subsets were examined by flowcytometry. n = 11, One-way ANOVA+ Bonferroni selected pairs post-test (SBA low vs. SBA high).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3275607&req=5

pone-0031160-g001: T-cell CD3 and CD28 expression and B-cell memory pool distribution.PBMCs were extracted from subjects with a MenC SBA titer <8 and matched controls one year post vaccination and levels of T-cell CD3 and CD28 expression B-cell memory subsets were examined by flowcytometry. n = 11, One-way ANOVA+ Bonferroni selected pairs post-test (SBA low vs. SBA high).
Mentions: To determine if any observed differences in anti-CD3 and anti-CD28 response were due to differences in CD3 and CD28 expression by CD4+ cells we analysed the level of surface expression of these markers. No significant difference was seen between the SBA low group (SBA titer <8) and the matched controls (Figure 1a).

Bottom Line: Despite the success of conjugate vaccination against meningococcal group C (MenC) disease, post-vaccination, some individuals still exhibit rapid waning of initially protective bactericidal antibody levels.The mechanism of this relative loss of humoral protection remains undetermined.We have found that healthy volunteers who lose protective MenC antibody levels one year after receipt of glycoconjugate vaccine exhibit no detectable cellular defect in polyclonal B- or T-cell activation, proliferation or the B-memory pool.

View Article: PubMed Central - PubMed

Affiliation: Department of Infection and Immunity, University of Sheffield Medical School, Sheffield, United Kingdom.

ABSTRACT
Despite the success of conjugate vaccination against meningococcal group C (MenC) disease, post-vaccination, some individuals still exhibit rapid waning of initially protective bactericidal antibody levels. The mechanism of this relative loss of humoral protection remains undetermined. In this report we have investigated the relationship between T- and B-cell activation and co-stimulation and the loss of protective antibody titers. We have found that healthy volunteers who lose protective MenC antibody levels one year after receipt of glycoconjugate vaccine exhibit no detectable cellular defect in polyclonal B- or T-cell activation, proliferation or the B-memory pool. This suggests that the processes underlying the more rapid loss of antibody levels are independent of defects in either initial T- or B-cell activation.

Show MeSH
Related in: MedlinePlus