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Revisiting the effect of acute P. falciparum malaria on Epstein-Barr virus: host balance in the setting of reduced malaria endemicity.

Jayasooriya S, Hislop A, Peng Y, Croom-Carter D, Jankey Y, Bell A, Dong T, Rowland-Jones S, Rickinson A, Walther M, Whittle H - PLoS ONE (2012)

Bottom Line: Previous studies in one such population suggested that acute P.falciparum infection impairs EBV-specific T-cell surveillance, allowing expansion of EBV infected B-cells from which BL derives.No significant changes were seen in EBV genome loads, percentage of EBV-specific CD8(+) T-cells and IFNγ producing T-cells in acute versus convalescent samples, nor any difference versus controls.Regression assays performed also no longer detected any impairment of EBV-specific T-cell surveillance.

View Article: PubMed Central - PubMed

Affiliation: Medical Research Council Laboratories, Fajara, The Gambia.

ABSTRACT
Burkitt's lymphoma (BL), an EBV-associated tumour, occurs at high incidence in populations where malaria is holoendemic. Previous studies in one such population suggested that acute P.falciparum infection impairs EBV-specific T-cell surveillance, allowing expansion of EBV infected B-cells from which BL derives. We re-examined the situation in the same area, The Gambia, after a reduction in malaria endemicity. Cellular immune responses to EBV were measured in children with uncomplicated malaria before (day 0) and after treatment (day 28), comparing EBV genome loads in blood and EBV-specific CD8(+) T-cell numbers (assayed by MHC Class I tetramers and IFNγ ELISPOTS) with those seen in age- and sex-matched healthy controls. No significant changes were seen in EBV genome loads, percentage of EBV-specific CD8(+) T-cells and IFNγ producing T-cells in acute versus convalescent samples, nor any difference versus controls. Regression assays performed also no longer detected any impairment of EBV-specific T-cell surveillance. Acute uncomplicated malaria infection no longer alters EBV-specific immune responses in children in The Gambia. Given the recent decline in malaria incidence in that country, we hypothesise that gross disturbance of the EBV-host balance may be a specific effect of acute malaria only in children with a history of chronic/recurrent malaria challenge.

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Related in: MedlinePlus

PD-1 expression correlates with CD38 expression during acute uncomplicated P. falciparum infection.PD-1 expression is shown on the y-axis and CD38 expression on the x- axis, each dot represents a single donor during acute (Day 0) uncomplicated P. falciparum malaria. There is a significant correlation between CD38 and PD-1 expression on CD8+ T-cells, A, and CD4+ T-cells, B.
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pone-0031142-g004: PD-1 expression correlates with CD38 expression during acute uncomplicated P. falciparum infection.PD-1 expression is shown on the y-axis and CD38 expression on the x- axis, each dot represents a single donor during acute (Day 0) uncomplicated P. falciparum malaria. There is a significant correlation between CD38 and PD-1 expression on CD8+ T-cells, A, and CD4+ T-cells, B.

Mentions: Consistent with recent data [22], we found that during acute disease (D0) CD38 expression, a marker of activation, significantly correlated with PD-1 expression on CD8+ T-cells (Spearman's correlation coefficient Rho = 0.5018, p = 0.0286, Figure 4A) and also CD4+ T-cells (Spearman's correlations coefficient Rho = 0.4881, p = 0.0399), supporting the hypothesis that PD-1 expression is associated with cellular activation and is not purely a marker of functional exhaustion in these cells. However, this was not found to be the case for CD19+ cells (Spearman's correlation Rho = 0.4298, and p = 0.0663), data not shown.


Revisiting the effect of acute P. falciparum malaria on Epstein-Barr virus: host balance in the setting of reduced malaria endemicity.

Jayasooriya S, Hislop A, Peng Y, Croom-Carter D, Jankey Y, Bell A, Dong T, Rowland-Jones S, Rickinson A, Walther M, Whittle H - PLoS ONE (2012)

PD-1 expression correlates with CD38 expression during acute uncomplicated P. falciparum infection.PD-1 expression is shown on the y-axis and CD38 expression on the x- axis, each dot represents a single donor during acute (Day 0) uncomplicated P. falciparum malaria. There is a significant correlation between CD38 and PD-1 expression on CD8+ T-cells, A, and CD4+ T-cells, B.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3275582&req=5

pone-0031142-g004: PD-1 expression correlates with CD38 expression during acute uncomplicated P. falciparum infection.PD-1 expression is shown on the y-axis and CD38 expression on the x- axis, each dot represents a single donor during acute (Day 0) uncomplicated P. falciparum malaria. There is a significant correlation between CD38 and PD-1 expression on CD8+ T-cells, A, and CD4+ T-cells, B.
Mentions: Consistent with recent data [22], we found that during acute disease (D0) CD38 expression, a marker of activation, significantly correlated with PD-1 expression on CD8+ T-cells (Spearman's correlation coefficient Rho = 0.5018, p = 0.0286, Figure 4A) and also CD4+ T-cells (Spearman's correlations coefficient Rho = 0.4881, p = 0.0399), supporting the hypothesis that PD-1 expression is associated with cellular activation and is not purely a marker of functional exhaustion in these cells. However, this was not found to be the case for CD19+ cells (Spearman's correlation Rho = 0.4298, and p = 0.0663), data not shown.

Bottom Line: Previous studies in one such population suggested that acute P.falciparum infection impairs EBV-specific T-cell surveillance, allowing expansion of EBV infected B-cells from which BL derives.No significant changes were seen in EBV genome loads, percentage of EBV-specific CD8(+) T-cells and IFNγ producing T-cells in acute versus convalescent samples, nor any difference versus controls.Regression assays performed also no longer detected any impairment of EBV-specific T-cell surveillance.

View Article: PubMed Central - PubMed

Affiliation: Medical Research Council Laboratories, Fajara, The Gambia.

ABSTRACT
Burkitt's lymphoma (BL), an EBV-associated tumour, occurs at high incidence in populations where malaria is holoendemic. Previous studies in one such population suggested that acute P.falciparum infection impairs EBV-specific T-cell surveillance, allowing expansion of EBV infected B-cells from which BL derives. We re-examined the situation in the same area, The Gambia, after a reduction in malaria endemicity. Cellular immune responses to EBV were measured in children with uncomplicated malaria before (day 0) and after treatment (day 28), comparing EBV genome loads in blood and EBV-specific CD8(+) T-cell numbers (assayed by MHC Class I tetramers and IFNγ ELISPOTS) with those seen in age- and sex-matched healthy controls. No significant changes were seen in EBV genome loads, percentage of EBV-specific CD8(+) T-cells and IFNγ producing T-cells in acute versus convalescent samples, nor any difference versus controls. Regression assays performed also no longer detected any impairment of EBV-specific T-cell surveillance. Acute uncomplicated malaria infection no longer alters EBV-specific immune responses in children in The Gambia. Given the recent decline in malaria incidence in that country, we hypothesise that gross disturbance of the EBV-host balance may be a specific effect of acute malaria only in children with a history of chronic/recurrent malaria challenge.

Show MeSH
Related in: MedlinePlus