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High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene.

Iqbal A, Lim YA, Surin J, Sim BL - PLoS ONE (2012)

Bottom Line: C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates).These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals.The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

ABSTRACT

Background: Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive patients.

Methodology/principal findings: In this study, 346 faecal samples collected from Malaysian HIV positive patients were genetically analysed via PCR targeting the 60 kDa glycoprotein (gp60) gene. Eighteen (5.2% of 346) isolates were determined as Cryptosporidium positive with 72.2% (of 18) identified as Cryptosporidium parvum whilst 27.7% as Cryptosporidium hominis. Further gp60 analysis revealed C. parvum belonging to subgenotypes IIaA13G1R1 (2 isolates), IIaA13G2R1 (2 isolates), IIaA14G2R1 (3 isolates), IIaA15G2R1 (5 isolates) and IIdA15G1R1 (1 isolate). C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates).

Conclusions/significance: These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals. The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals.

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Related in: MedlinePlus

Phylogenetic analysis of gp60 sequence data representing Cryptosporidium hominis from HIV patients using Bayesian inference (BI).Sequence from the present study as well as reference sequences representing C. hominis subtypes (acquired from GenBank) are indicated. Posterior probabilities are indicated at all major nodes. C. hominis genotype Ia accession numbers HQ631406 and HQ631408 and genotype Ib accession numbers HQ631409 and HQ631410 have been reported in another publication by our group [12].
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pone-0031139-g003: Phylogenetic analysis of gp60 sequence data representing Cryptosporidium hominis from HIV patients using Bayesian inference (BI).Sequence from the present study as well as reference sequences representing C. hominis subtypes (acquired from GenBank) are indicated. Posterior probabilities are indicated at all major nodes. C. hominis genotype Ia accession numbers HQ631406 and HQ631408 and genotype Ib accession numbers HQ631409 and HQ631410 have been reported in another publication by our group [12].

Mentions: Phylogenetic analysis of sequence data of gp60 C. parvum genotypes IIa and IId isolates of HIV patients were conducted by Bayesian Interferance (BI) (Figure 2). The relationship of gp60 genotypes of Cryptosporidium isolates and reference sequences of C. parvum genotypes recognized the presence of genotypes IIa and IId in HIV patients. Cryptosporidium isolates of C. parvum genotypes IIa (HQ631411, HQ631412, HQ631413, HQ631414, HQ631415, HQ631416, HQ631417, HQ631418, HQ631419, HQ631420, HQ631421 and HQ631422) were clustered with reference sequence of C. parvum genotypes IIa (A14G2R1, A17G1R1, A19G3R1, A20G3R1, A22G3R1 and A23G3R1) with high internode value (i.e., 0.93). Whilst isolate of C. parvum genotypes IId (HQ631423) was grouped with reference sequences of C. parvum genotypes IId (A17G2R1, A18G2R1, A19G2R1, A21G2R1, A22G2R1) with highest internode value (i.e., 1.00). Analysis of the gp60 C. hominis genotypes Ia and Ib isolates of HIV patients were built by means of Bayesian Interferance (BI) (Figure 3). The association of gp60 genotypes of Cryptosporidium isolates and reference sequences of gp60 C. hominis genotypes predicted the presence of genotypes Ia and Ib in this study. Cryptosporidium isolates of C. hominis genotypes Ia (HQ631406, HQ631407 and HQ631408) were grouped with reference sequences of C. hominis genotype Ia (A12G1, A12G1R1, A14R1, A15R1, A19R1, A21R1, A22R1, A23R1, A23R2, A24R1 and A27R1) with high internode value (i.e., 1.00). Furthermore, isolates of C. hominis genotype Ib (HQ631409 and HQ631410) were clustered with reference sequences of C. hominis genotype Ib (A9G3R2, A18G1R4, A23G2R2) with 0.88 internode value.


High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene.

Iqbal A, Lim YA, Surin J, Sim BL - PLoS ONE (2012)

Phylogenetic analysis of gp60 sequence data representing Cryptosporidium hominis from HIV patients using Bayesian inference (BI).Sequence from the present study as well as reference sequences representing C. hominis subtypes (acquired from GenBank) are indicated. Posterior probabilities are indicated at all major nodes. C. hominis genotype Ia accession numbers HQ631406 and HQ631408 and genotype Ib accession numbers HQ631409 and HQ631410 have been reported in another publication by our group [12].
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3275556&req=5

pone-0031139-g003: Phylogenetic analysis of gp60 sequence data representing Cryptosporidium hominis from HIV patients using Bayesian inference (BI).Sequence from the present study as well as reference sequences representing C. hominis subtypes (acquired from GenBank) are indicated. Posterior probabilities are indicated at all major nodes. C. hominis genotype Ia accession numbers HQ631406 and HQ631408 and genotype Ib accession numbers HQ631409 and HQ631410 have been reported in another publication by our group [12].
Mentions: Phylogenetic analysis of sequence data of gp60 C. parvum genotypes IIa and IId isolates of HIV patients were conducted by Bayesian Interferance (BI) (Figure 2). The relationship of gp60 genotypes of Cryptosporidium isolates and reference sequences of C. parvum genotypes recognized the presence of genotypes IIa and IId in HIV patients. Cryptosporidium isolates of C. parvum genotypes IIa (HQ631411, HQ631412, HQ631413, HQ631414, HQ631415, HQ631416, HQ631417, HQ631418, HQ631419, HQ631420, HQ631421 and HQ631422) were clustered with reference sequence of C. parvum genotypes IIa (A14G2R1, A17G1R1, A19G3R1, A20G3R1, A22G3R1 and A23G3R1) with high internode value (i.e., 0.93). Whilst isolate of C. parvum genotypes IId (HQ631423) was grouped with reference sequences of C. parvum genotypes IId (A17G2R1, A18G2R1, A19G2R1, A21G2R1, A22G2R1) with highest internode value (i.e., 1.00). Analysis of the gp60 C. hominis genotypes Ia and Ib isolates of HIV patients were built by means of Bayesian Interferance (BI) (Figure 3). The association of gp60 genotypes of Cryptosporidium isolates and reference sequences of gp60 C. hominis genotypes predicted the presence of genotypes Ia and Ib in this study. Cryptosporidium isolates of C. hominis genotypes Ia (HQ631406, HQ631407 and HQ631408) were grouped with reference sequences of C. hominis genotype Ia (A12G1, A12G1R1, A14R1, A15R1, A19R1, A21R1, A22R1, A23R1, A23R2, A24R1 and A27R1) with high internode value (i.e., 1.00). Furthermore, isolates of C. hominis genotype Ib (HQ631409 and HQ631410) were clustered with reference sequences of C. hominis genotype Ib (A9G3R2, A18G1R4, A23G2R2) with 0.88 internode value.

Bottom Line: C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates).These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals.The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

ABSTRACT

Background: Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive patients.

Methodology/principal findings: In this study, 346 faecal samples collected from Malaysian HIV positive patients were genetically analysed via PCR targeting the 60 kDa glycoprotein (gp60) gene. Eighteen (5.2% of 346) isolates were determined as Cryptosporidium positive with 72.2% (of 18) identified as Cryptosporidium parvum whilst 27.7% as Cryptosporidium hominis. Further gp60 analysis revealed C. parvum belonging to subgenotypes IIaA13G1R1 (2 isolates), IIaA13G2R1 (2 isolates), IIaA14G2R1 (3 isolates), IIaA15G2R1 (5 isolates) and IIdA15G1R1 (1 isolate). C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates).

Conclusions/significance: These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals. The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals.

Show MeSH
Related in: MedlinePlus