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Phylogenetic analysis of strains of Orf virus isolated from two outbreaks of the disease in sheep in Greece.

Billinis C, Mavrogianni VS, Spyrou V, Fthenakis GC - Virol. J. (2012)

Bottom Line: The homology of the nucleotide and amino-acid sequences between the two Greek isolates was 99.0% and 98.8%, respectively.We suggest that there can be differences between strains based on their geographical origin.However, differences in the origin of strains or in the clinical presentation of the disease may not be associated with their pathogenicity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Veterinary Faculty, University of Thessaly, Karditsa, Greece.

ABSTRACT

Background: Although orf is endemic around the world, there are few descriptions of Orf virus strains and comparisons of these strains. We report the sequence and phylogenetic analysis of the partial B2L gene of Orf virus from two outbreaks of the disease in Greece. The first was an outbreak of genital form of the disease in a flock imported from France, whilst the second was an outbreak of the disease in the udder skin of ewes and around the mouth of lambs in an indigenous flock.

Results: Phylogenetic analysis was performed on a part (498 bp) of the B2L gene of 35 Parapoxvirus isolates, including the two Orf virus isolates recovered from each of the two outbreaks in the present study. This analysis revealed that the maximum nucleotide and amino-acid variation amongst Orf virus strains worldwide (n = 33) was 8.1% and 9.6%, respectively. The homology of the nucleotide and amino-acid sequences between the two Greek isolates was 99.0% and 98.8%, respectively. The two Greek isolates clustered only with Orf virus strains.

Conclusions: We suggest that there can be differences between strains based on their geographical origin. However, differences in the origin of strains or in the clinical presentation of the disease may not be associated with their pathogenicity. More work is required to determine if differing clinical presentations are linked to viral strain differences or if other factors, e.g., flock immunity, method of exposure or genetic susceptibility, are more important to determine the clinical presentation of the infection.

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A neighbour-joining phylogenetic tree, using Kimura-2 parameter distance matrix, from 35 (including those in present study) Parapoxvirus strain sequences (partial sequences of B2L gene). GenBank accession numbers and geographic origin of strains are shown. Bootstrap values (%) are represented at each tree node. Node support was assessed with 1,000 bootstrap pseudo-replicates.
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Figure 3: A neighbour-joining phylogenetic tree, using Kimura-2 parameter distance matrix, from 35 (including those in present study) Parapoxvirus strain sequences (partial sequences of B2L gene). GenBank accession numbers and geographic origin of strains are shown. Bootstrap values (%) are represented at each tree node. Node support was assessed with 1,000 bootstrap pseudo-replicates.

Mentions: The partial sequence of the major envelope gene of the two strains of Orf virus, i.e. 'GRE-1 genital 2003' and 'GRE-2 teat 2004', were submitted to GenBank with accession numbers JN368482 and JN368483, respectively. Nucleotide sequences from other Parapoxvirus isolates were retrieved from Genbank (NCBI). Phylogenetic and molecular evolutionary analyses were conducted using program MEGA 3.1 [21]. A neighbour-joining phylogenetic tree using Kimura-2 parameter distance matrix [22] was inferred from sequence of 35 Parapoxvirus strains; these included a Bovine papular stomatitis virus strain, Pseudocowpox virus strain, 31 Orf virus strains previously isolated by other researchers and the two strains isolated in the present study (Figure. 3). Node support was assessed with 1,000 bootstrap pseudo-replicates. Detailed information of Parapoxvirus strains analysed is in Table 1.


Phylogenetic analysis of strains of Orf virus isolated from two outbreaks of the disease in sheep in Greece.

Billinis C, Mavrogianni VS, Spyrou V, Fthenakis GC - Virol. J. (2012)

A neighbour-joining phylogenetic tree, using Kimura-2 parameter distance matrix, from 35 (including those in present study) Parapoxvirus strain sequences (partial sequences of B2L gene). GenBank accession numbers and geographic origin of strains are shown. Bootstrap values (%) are represented at each tree node. Node support was assessed with 1,000 bootstrap pseudo-replicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3275544&req=5

Figure 3: A neighbour-joining phylogenetic tree, using Kimura-2 parameter distance matrix, from 35 (including those in present study) Parapoxvirus strain sequences (partial sequences of B2L gene). GenBank accession numbers and geographic origin of strains are shown. Bootstrap values (%) are represented at each tree node. Node support was assessed with 1,000 bootstrap pseudo-replicates.
Mentions: The partial sequence of the major envelope gene of the two strains of Orf virus, i.e. 'GRE-1 genital 2003' and 'GRE-2 teat 2004', were submitted to GenBank with accession numbers JN368482 and JN368483, respectively. Nucleotide sequences from other Parapoxvirus isolates were retrieved from Genbank (NCBI). Phylogenetic and molecular evolutionary analyses were conducted using program MEGA 3.1 [21]. A neighbour-joining phylogenetic tree using Kimura-2 parameter distance matrix [22] was inferred from sequence of 35 Parapoxvirus strains; these included a Bovine papular stomatitis virus strain, Pseudocowpox virus strain, 31 Orf virus strains previously isolated by other researchers and the two strains isolated in the present study (Figure. 3). Node support was assessed with 1,000 bootstrap pseudo-replicates. Detailed information of Parapoxvirus strains analysed is in Table 1.

Bottom Line: The homology of the nucleotide and amino-acid sequences between the two Greek isolates was 99.0% and 98.8%, respectively.We suggest that there can be differences between strains based on their geographical origin.However, differences in the origin of strains or in the clinical presentation of the disease may not be associated with their pathogenicity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Veterinary Faculty, University of Thessaly, Karditsa, Greece.

ABSTRACT

Background: Although orf is endemic around the world, there are few descriptions of Orf virus strains and comparisons of these strains. We report the sequence and phylogenetic analysis of the partial B2L gene of Orf virus from two outbreaks of the disease in Greece. The first was an outbreak of genital form of the disease in a flock imported from France, whilst the second was an outbreak of the disease in the udder skin of ewes and around the mouth of lambs in an indigenous flock.

Results: Phylogenetic analysis was performed on a part (498 bp) of the B2L gene of 35 Parapoxvirus isolates, including the two Orf virus isolates recovered from each of the two outbreaks in the present study. This analysis revealed that the maximum nucleotide and amino-acid variation amongst Orf virus strains worldwide (n = 33) was 8.1% and 9.6%, respectively. The homology of the nucleotide and amino-acid sequences between the two Greek isolates was 99.0% and 98.8%, respectively. The two Greek isolates clustered only with Orf virus strains.

Conclusions: We suggest that there can be differences between strains based on their geographical origin. However, differences in the origin of strains or in the clinical presentation of the disease may not be associated with their pathogenicity. More work is required to determine if differing clinical presentations are linked to viral strain differences or if other factors, e.g., flock immunity, method of exposure or genetic susceptibility, are more important to determine the clinical presentation of the infection.

Show MeSH
Related in: MedlinePlus