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Baicalein antagonizes rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to Parkinsonism.

Song JX, Choi MY, Wong KC, Chung WW, Sze SC, Ng TB, Zhang KY - Chin Med (2012)

Bottom Line: Baicalein significantly increased viability and decreased rotenone-induced death of SH-SY5Y cells in a dose-dependent manner.Baicalein antagonized rotenone-induced overproduction of ROS, loss of ΔΨm, the increased expression of Bax, cleaved caspase-3 and phosphorylated ERK1/2 and the decreased expression of Bcl-2.The antioxidative effect, mitochondrial protection and modulation of anti-and pro-apoptotic proteins are related to the neuroprotective effects of baicalein against rotenone induced cell death in SH-SY5Y cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Chinese Medicine, The University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong SAR, China. zhang.yanbo@yahoo.com.

ABSTRACT

Background: Two active compounds, baicalein and its glycoside baicalin were found in the dried root of Scutellaria baicalensis Georgi, and reported to be neuroprotective in vitro and in vivo. This study aims to evaluate the protective effects of baicalein on the rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to parkinsonism.

Methods: Cell viability and cytotoxicity were determined by MTT assay. The degree of nuclear apoptosis was evaluated with a fluorescent DNA-binding probe Hoechst 33258. The production of reactive oxidative species (ROS) and loss of mitochondrial membrane potential (ΔΨm) were determined by fluorescent staining with DCFH-DA and Rhodanmine 123, respectively. The expression of Bax, Bcl-2, cleaved caspase-3 and phosphorylated ERK1/2 was determined by the Western blots.

Results: Baicalein significantly increased viability and decreased rotenone-induced death of SH-SY5Y cells in a dose-dependent manner. Pre- and subsequent co-treatment with baicalein preserved the cell morphology and attenuated the nuclear apoptotic characteristics triggered by rotenone. Baicalein antagonized rotenone-induced overproduction of ROS, loss of ΔΨm, the increased expression of Bax, cleaved caspase-3 and phosphorylated ERK1/2 and the decreased expression of Bcl-2.

Conclusion: The antioxidative effect, mitochondrial protection and modulation of anti-and pro-apoptotic proteins are related to the neuroprotective effects of baicalein against rotenone induced cell death in SH-SY5Y cells.

No MeSH data available.


Related in: MedlinePlus

Effects of baicalein and baicalin on rotenone-induced cell death in SH-SY5Y cells. Cells were incubated with increasing concentrations of rotenone (A), baicalein and baicalin (B) respectively for 24 hours in serum-free medium (n = 6, *P < 0.01 versus control). Cells were pretreated with baicalein (C) or baicalin (D) for 1 hour and then cotreated with 20 μM rotenone for 24 hours in serum-free medium (n = 6, #P < 0.01 versus control, *P < 0.01 versus rotenone treatment). E: The morphological change was visualized by phase-contrast imaging. Scale bar: 50 μm.
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Figure 2: Effects of baicalein and baicalin on rotenone-induced cell death in SH-SY5Y cells. Cells were incubated with increasing concentrations of rotenone (A), baicalein and baicalin (B) respectively for 24 hours in serum-free medium (n = 6, *P < 0.01 versus control). Cells were pretreated with baicalein (C) or baicalin (D) for 1 hour and then cotreated with 20 μM rotenone for 24 hours in serum-free medium (n = 6, #P < 0.01 versus control, *P < 0.01 versus rotenone treatment). E: The morphological change was visualized by phase-contrast imaging. Scale bar: 50 μm.

Mentions: The cytotoxicity of rotenone, baicalein and baicalin were determined by MTT assay, Figure 2A shows that the cell viability was decreased in a dose-dependent manner (P < 0.01) by the treatment with rotenone for 24 hours. Rotenone (20 μM) triggered about 50% cell death and this concentration was chosen for subsequent experiments. Both baicalein and baicalin showed no cytotoxicity at the concentrations ranged 10-100 μM. Figure 2B shows that baicalein increased cell viability by 20-40% (P < 0.01) at all the tested concentrations, compared with the control.


Baicalein antagonizes rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to Parkinsonism.

Song JX, Choi MY, Wong KC, Chung WW, Sze SC, Ng TB, Zhang KY - Chin Med (2012)

Effects of baicalein and baicalin on rotenone-induced cell death in SH-SY5Y cells. Cells were incubated with increasing concentrations of rotenone (A), baicalein and baicalin (B) respectively for 24 hours in serum-free medium (n = 6, *P < 0.01 versus control). Cells were pretreated with baicalein (C) or baicalin (D) for 1 hour and then cotreated with 20 μM rotenone for 24 hours in serum-free medium (n = 6, #P < 0.01 versus control, *P < 0.01 versus rotenone treatment). E: The morphological change was visualized by phase-contrast imaging. Scale bar: 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3275529&req=5

Figure 2: Effects of baicalein and baicalin on rotenone-induced cell death in SH-SY5Y cells. Cells were incubated with increasing concentrations of rotenone (A), baicalein and baicalin (B) respectively for 24 hours in serum-free medium (n = 6, *P < 0.01 versus control). Cells were pretreated with baicalein (C) or baicalin (D) for 1 hour and then cotreated with 20 μM rotenone for 24 hours in serum-free medium (n = 6, #P < 0.01 versus control, *P < 0.01 versus rotenone treatment). E: The morphological change was visualized by phase-contrast imaging. Scale bar: 50 μm.
Mentions: The cytotoxicity of rotenone, baicalein and baicalin were determined by MTT assay, Figure 2A shows that the cell viability was decreased in a dose-dependent manner (P < 0.01) by the treatment with rotenone for 24 hours. Rotenone (20 μM) triggered about 50% cell death and this concentration was chosen for subsequent experiments. Both baicalein and baicalin showed no cytotoxicity at the concentrations ranged 10-100 μM. Figure 2B shows that baicalein increased cell viability by 20-40% (P < 0.01) at all the tested concentrations, compared with the control.

Bottom Line: Baicalein significantly increased viability and decreased rotenone-induced death of SH-SY5Y cells in a dose-dependent manner.Baicalein antagonized rotenone-induced overproduction of ROS, loss of ΔΨm, the increased expression of Bax, cleaved caspase-3 and phosphorylated ERK1/2 and the decreased expression of Bcl-2.The antioxidative effect, mitochondrial protection and modulation of anti-and pro-apoptotic proteins are related to the neuroprotective effects of baicalein against rotenone induced cell death in SH-SY5Y cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Chinese Medicine, The University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong SAR, China. zhang.yanbo@yahoo.com.

ABSTRACT

Background: Two active compounds, baicalein and its glycoside baicalin were found in the dried root of Scutellaria baicalensis Georgi, and reported to be neuroprotective in vitro and in vivo. This study aims to evaluate the protective effects of baicalein on the rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to parkinsonism.

Methods: Cell viability and cytotoxicity were determined by MTT assay. The degree of nuclear apoptosis was evaluated with a fluorescent DNA-binding probe Hoechst 33258. The production of reactive oxidative species (ROS) and loss of mitochondrial membrane potential (ΔΨm) were determined by fluorescent staining with DCFH-DA and Rhodanmine 123, respectively. The expression of Bax, Bcl-2, cleaved caspase-3 and phosphorylated ERK1/2 was determined by the Western blots.

Results: Baicalein significantly increased viability and decreased rotenone-induced death of SH-SY5Y cells in a dose-dependent manner. Pre- and subsequent co-treatment with baicalein preserved the cell morphology and attenuated the nuclear apoptotic characteristics triggered by rotenone. Baicalein antagonized rotenone-induced overproduction of ROS, loss of ΔΨm, the increased expression of Bax, cleaved caspase-3 and phosphorylated ERK1/2 and the decreased expression of Bcl-2.

Conclusion: The antioxidative effect, mitochondrial protection and modulation of anti-and pro-apoptotic proteins are related to the neuroprotective effects of baicalein against rotenone induced cell death in SH-SY5Y cells.

No MeSH data available.


Related in: MedlinePlus