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Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility.

Szczepańska M, Wirstlein P, Skrzypczak J, Jagodziński PP - Reprod. Biol. Endocrinol. (2012)

Bottom Line: A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility.However, we did not observe significant differences in DNMT1 and DNMT3B transcript levels between these investigated groups of women.We confirmed that reduced HOXA11 expression may contribute to endometriosis-associated infertility.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Obstetrics, Gynecology and Gynecological Oncology, Division of Reproduction, Poznan Medical University of Sciences Poland.

ABSTRACT

Background: A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility.

Methods: Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from eighteen infertile women with minimal endometriosis, sixteen healthy fertile women and sixteen infertile women with fallopian tubal occlusion from the Polish population. We also evaluated transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in these groups of women.

Results: There were significantly lower levels of HOXA11 transcripts (p = 0.003, p = 0.041) and protein (p = 0.004, p = 0.001) in women with endometriosis as compared to fertile women and infertile women with tubal occlusion. Moreover, we found significantly higher methylation levels of the CpG region in the first exon of HOXA11 in infertile women with endometriosis compared with fertile women (p < 0.001) and infertile women with tubal occlusion (p < 0.001). We also observed significantly increased levels of DNMT3A transcript in women with endometriosis than fertile women (p = 0.044) and infertile women with tubal occlusion (p = 0.047). However, we did not observe significant differences in DNMT1 and DNMT3B transcript levels between these investigated groups of women.

Conclusions: We confirmed that reduced HOXA11 expression may contribute to endometriosis-associated infertility. Moreover, we found that DNA hypermethylation can be one of the possible molecular mechanisms causing a decrease in HOXA11 expression in the eutopic mid-secretory endometrium in infertile women with endometriosis.

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DNMT1 (A), DNMT3A (B) and DNMT3B (C) transcript levels in eutopic mid-luteal endometrium from infertile women with endometriosis, fertile women and infertile women with tubal occlusion. The eutopic endometrial tissue from women with endometriosis, fertile women and infertile women with tubal occlusion was respectively obtained by pipelle or hysteroscopic biopsy during the implantation window, followed by total RNA isolation. RNA was reverse-transcribed and DNMT1, DNMT3A and DNMT3B cDNAs were investigated by RQ-PCR relative quantification analysis. To normalize the quantity of transcripts in each sample, DNMTs mRNA levels were normalised to the amount of GAPDH and ACTB cDNA. The amounts of DNMTs mRNA were expressed as the multiplicity of these cDNA copies in the calibrator. The boxes and the middle lines correspond to the values from the lower to upper quartiles and medians, respectively.
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Figure 2: DNMT1 (A), DNMT3A (B) and DNMT3B (C) transcript levels in eutopic mid-luteal endometrium from infertile women with endometriosis, fertile women and infertile women with tubal occlusion. The eutopic endometrial tissue from women with endometriosis, fertile women and infertile women with tubal occlusion was respectively obtained by pipelle or hysteroscopic biopsy during the implantation window, followed by total RNA isolation. RNA was reverse-transcribed and DNMT1, DNMT3A and DNMT3B cDNAs were investigated by RQ-PCR relative quantification analysis. To normalize the quantity of transcripts in each sample, DNMTs mRNA levels were normalised to the amount of GAPDH and ACTB cDNA. The amounts of DNMTs mRNA were expressed as the multiplicity of these cDNA copies in the calibrator. The boxes and the middle lines correspond to the values from the lower to upper quartiles and medians, respectively.

Mentions: RQ-PCR analysis showed significantly increased levels of DNMT3A transcript in eutopic mid-secretory endometrium from women with endometriosis compared to fertile women (p = 0.044) and women with tubal occlusion (p = 0.047) (Table 3 and Figure 2A). However, we did not observe significant differences in DNMT1 (p = 0.621, p = 0.470) and DNMT3B (p = 0.717, p = 0.100) transcript levels between the investigated groups (Table 3 and Figures 2B and 2C).


Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility.

Szczepańska M, Wirstlein P, Skrzypczak J, Jagodziński PP - Reprod. Biol. Endocrinol. (2012)

DNMT1 (A), DNMT3A (B) and DNMT3B (C) transcript levels in eutopic mid-luteal endometrium from infertile women with endometriosis, fertile women and infertile women with tubal occlusion. The eutopic endometrial tissue from women with endometriosis, fertile women and infertile women with tubal occlusion was respectively obtained by pipelle or hysteroscopic biopsy during the implantation window, followed by total RNA isolation. RNA was reverse-transcribed and DNMT1, DNMT3A and DNMT3B cDNAs were investigated by RQ-PCR relative quantification analysis. To normalize the quantity of transcripts in each sample, DNMTs mRNA levels were normalised to the amount of GAPDH and ACTB cDNA. The amounts of DNMTs mRNA were expressed as the multiplicity of these cDNA copies in the calibrator. The boxes and the middle lines correspond to the values from the lower to upper quartiles and medians, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3275521&req=5

Figure 2: DNMT1 (A), DNMT3A (B) and DNMT3B (C) transcript levels in eutopic mid-luteal endometrium from infertile women with endometriosis, fertile women and infertile women with tubal occlusion. The eutopic endometrial tissue from women with endometriosis, fertile women and infertile women with tubal occlusion was respectively obtained by pipelle or hysteroscopic biopsy during the implantation window, followed by total RNA isolation. RNA was reverse-transcribed and DNMT1, DNMT3A and DNMT3B cDNAs were investigated by RQ-PCR relative quantification analysis. To normalize the quantity of transcripts in each sample, DNMTs mRNA levels were normalised to the amount of GAPDH and ACTB cDNA. The amounts of DNMTs mRNA were expressed as the multiplicity of these cDNA copies in the calibrator. The boxes and the middle lines correspond to the values from the lower to upper quartiles and medians, respectively.
Mentions: RQ-PCR analysis showed significantly increased levels of DNMT3A transcript in eutopic mid-secretory endometrium from women with endometriosis compared to fertile women (p = 0.044) and women with tubal occlusion (p = 0.047) (Table 3 and Figure 2A). However, we did not observe significant differences in DNMT1 (p = 0.621, p = 0.470) and DNMT3B (p = 0.717, p = 0.100) transcript levels between the investigated groups (Table 3 and Figures 2B and 2C).

Bottom Line: A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility.However, we did not observe significant differences in DNMT1 and DNMT3B transcript levels between these investigated groups of women.We confirmed that reduced HOXA11 expression may contribute to endometriosis-associated infertility.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Obstetrics, Gynecology and Gynecological Oncology, Division of Reproduction, Poznan Medical University of Sciences Poland.

ABSTRACT

Background: A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility.

Methods: Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from eighteen infertile women with minimal endometriosis, sixteen healthy fertile women and sixteen infertile women with fallopian tubal occlusion from the Polish population. We also evaluated transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in these groups of women.

Results: There were significantly lower levels of HOXA11 transcripts (p = 0.003, p = 0.041) and protein (p = 0.004, p = 0.001) in women with endometriosis as compared to fertile women and infertile women with tubal occlusion. Moreover, we found significantly higher methylation levels of the CpG region in the first exon of HOXA11 in infertile women with endometriosis compared with fertile women (p < 0.001) and infertile women with tubal occlusion (p < 0.001). We also observed significantly increased levels of DNMT3A transcript in women with endometriosis than fertile women (p = 0.044) and infertile women with tubal occlusion (p = 0.047). However, we did not observe significant differences in DNMT1 and DNMT3B transcript levels between these investigated groups of women.

Conclusions: We confirmed that reduced HOXA11 expression may contribute to endometriosis-associated infertility. Moreover, we found that DNA hypermethylation can be one of the possible molecular mechanisms causing a decrease in HOXA11 expression in the eutopic mid-secretory endometrium in infertile women with endometriosis.

Show MeSH
Related in: MedlinePlus