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Complete genome sequence of Staphylothermus hellenicus P8.

Anderson I, Wirth R, Lucas S, Copeland A, Lapidus A, Cheng JF, Goodwin L, Pitluck S, Davenport K, Detter JC, Han C, Tapia R, Land M, Hauser L, Pati A, Mikhailova N, Woyke T, Klenk HP, Kyrpides N, Ivanova N - Stand Genomic Sci (2011)

Bottom Line: Staphylothermus hellenicus belongs to the order Desulfurococcales within the archaeal phylum Crenarchaeota.It is a hyperthermophilic, anaerobic heterotroph.Here we describe the features of this organism together with the complete genome sequence and annotation.

View Article: PubMed Central - PubMed

ABSTRACT
Staphylothermus hellenicus belongs to the order Desulfurococcales within the archaeal phylum Crenarchaeota. Strain P8(T) is the type strain of the species and was isolated from a shallow hydrothermal vent system at Palaeochori Bay, Milos, Greece. It is a hyperthermophilic, anaerobic heterotroph. Here we describe the features of this organism together with the complete genome sequence and annotation. The 1,580,347 bp genome with its 1,668 protein-coding and 48 RNA genes was sequenced as part of a DOE Joint Genome Institute (JGI) Laboratory Sequencing Program (LSP) project.

No MeSH data available.


Related in: MedlinePlus

Scanning electron micrograph of S. hellenicus P8T.
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f2: Scanning electron micrograph of S. hellenicus P8T.

Mentions: S. hellenicus was isolated from sediment at Palaeochori Bay, Milos, Greece [1]. For isolation, 1 ml of sediment was added to half-strength SME medium [11] with 2% elemental sulfur and incubated at 90°C under H2/CO2. Colonies were isolated on plates with the same medium and with 1% Phytagel and 2-3% sodium alginate added [1]. S. hellenicus is a regular-shaped coccus (Figure 2) which can form large aggregates of up to fifty cells, similar to S. marinus [1,12]. No flagella were observed and cells were nonmotile. The temperature range for growth of S. hellenicus is 70-90°C, with an optimum at 85°C [1]. The salinity range was from 2% to 8% NaCl, and the optimum was 4% NaCl [1]. The pH range for growth was from 4.5 to 7.5. The optimum pH was 6.0 [1]. S. hellenicus is a strict anaerobe, and can grow under H2/CO2 or N2/CO2 [1]. It is a heterotroph which grows well on yeast extract but poorly on peptone [1]. Many carbon sources were tested, but no growth was observed, showing that a complex nutrient source is required [1]. Elemental sulfur was required for growth [1]. The features of the organism are listed in Table 1.


Complete genome sequence of Staphylothermus hellenicus P8.

Anderson I, Wirth R, Lucas S, Copeland A, Lapidus A, Cheng JF, Goodwin L, Pitluck S, Davenport K, Detter JC, Han C, Tapia R, Land M, Hauser L, Pati A, Mikhailova N, Woyke T, Klenk HP, Kyrpides N, Ivanova N - Stand Genomic Sci (2011)

Scanning electron micrograph of S. hellenicus P8T.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3236042&req=5

f2: Scanning electron micrograph of S. hellenicus P8T.
Mentions: S. hellenicus was isolated from sediment at Palaeochori Bay, Milos, Greece [1]. For isolation, 1 ml of sediment was added to half-strength SME medium [11] with 2% elemental sulfur and incubated at 90°C under H2/CO2. Colonies were isolated on plates with the same medium and with 1% Phytagel and 2-3% sodium alginate added [1]. S. hellenicus is a regular-shaped coccus (Figure 2) which can form large aggregates of up to fifty cells, similar to S. marinus [1,12]. No flagella were observed and cells were nonmotile. The temperature range for growth of S. hellenicus is 70-90°C, with an optimum at 85°C [1]. The salinity range was from 2% to 8% NaCl, and the optimum was 4% NaCl [1]. The pH range for growth was from 4.5 to 7.5. The optimum pH was 6.0 [1]. S. hellenicus is a strict anaerobe, and can grow under H2/CO2 or N2/CO2 [1]. It is a heterotroph which grows well on yeast extract but poorly on peptone [1]. Many carbon sources were tested, but no growth was observed, showing that a complex nutrient source is required [1]. Elemental sulfur was required for growth [1]. The features of the organism are listed in Table 1.

Bottom Line: Staphylothermus hellenicus belongs to the order Desulfurococcales within the archaeal phylum Crenarchaeota.It is a hyperthermophilic, anaerobic heterotroph.Here we describe the features of this organism together with the complete genome sequence and annotation.

View Article: PubMed Central - PubMed

ABSTRACT
Staphylothermus hellenicus belongs to the order Desulfurococcales within the archaeal phylum Crenarchaeota. Strain P8(T) is the type strain of the species and was isolated from a shallow hydrothermal vent system at Palaeochori Bay, Milos, Greece. It is a hyperthermophilic, anaerobic heterotroph. Here we describe the features of this organism together with the complete genome sequence and annotation. The 1,580,347 bp genome with its 1,668 protein-coding and 48 RNA genes was sequenced as part of a DOE Joint Genome Institute (JGI) Laboratory Sequencing Program (LSP) project.

No MeSH data available.


Related in: MedlinePlus