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Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse.

Wang Y, Osterbur DL, Megaw PL, Tosini G, Fukuhara C, Green CB, Besharse JC - BMC Dev. Biol. (2001)

Bottom Line: Nocturnin was originally identified by differential display as a circadian clock regulated gene with high expression at night in photoreceptors of the African clawed frog, Xenopus laevis.Furthermore, mNoc exhibits circadian rhythmicity of mRNA abundance with peak levels at the time of light offset in the retina, spleen, heart, kidney and liver.The widespread expression and rhythmicity of mNoc mRNA parallels the widespread expression of other circadian clock genes in mammalian tissues, and suggests that nocturnin plays an important role in clock function or as a circadian clock effector.

View Article: PubMed Central - HTML - PubMed

Affiliation: PEL-FREEZ Clinical Systems, LLC, 9099 North Deerbrook Trail, Brown Deer, WI 53223, USA. ywang@pel-freez.com

ABSTRACT

Background: Nocturnin was originally identified by differential display as a circadian clock regulated gene with high expression at night in photoreceptors of the African clawed frog, Xenopus laevis. Although encoding a novel protein, the nocturnin cDNA had strong sequence similarity with a C-terminal domain of the yeast transcription factor CCR4, and with mouse and human ESTs. Since its original identification others have cloned mouse and human homologues of nocturnin/CCR4, and we have cloned a full-length cDNA from mouse retina, along with partial cDNAs from human, cow and chicken. The goal of this study was to determine the temporal pattern of nocturnin mRNA expression in multiple tissues of the mouse.

Results: cDNA sequence analysis revealed a high degree of conservation among vertebrate nocturnin/CCR4 homologues along with a possible homologue in Drosophila. Northern analysis of mRNA in C3H/He and C57/Bl6 mice revealed that the mNoc gene is expressed in a broad range of tissues, with greatest abundance in liver, kidney and testis. mNoc is also expressed in multiple brain regions including suprachiasmatic nucleus and pineal gland. Furthermore, mNoc exhibits circadian rhythmicity of mRNA abundance with peak levels at the time of light offset in the retina, spleen, heart, kidney and liver.

Conclusion: The widespread expression and rhythmicity of mNoc mRNA parallels the widespread expression of other circadian clock genes in mammalian tissues, and suggests that nocturnin plays an important role in clock function or as a circadian clock effector.

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Comparison of the conceptual amino acid sequences of nocturnin homologues from chicken (CNOC), cow (BNOC), rat, (RNOC), Xenopus (XNOC), mouse (MNOC), human (hNoc) and Drosophila (DNOC). Sequences were analyzed using the Clustal W Alignment procedure. Gaps indicated with dots are inserted to achieve optimum alignment. Dark gray and light gray highlights indicate amino acid identities and similarities respectively. The horizontal bar marks the position of the heptad leucine repeat in Xenopus and the asterisks indicate the position of the leucines. The positions of introns 1 and 2 based on the Xenopus gene [36] are indicated by arrows. Note that both mouse [39] and human (from data in public databases of the National Library of Medicine) appear to have a similar gene structure based on 3 exons. The XNOC sequence is from GenBank accession number U74761, HNOC is from NP036250.1 [39], and DNOC is from AAF54601.1 [40]. Our complete mNoc cDNA from retina (accession number AF199491) has the same coding sequence as that reported earlier from liver [38,39]. The cNoc (AF199498), bNoc (AF199497), and rNoc (AF199495) partial sequences are from PCR amplified DNA segments.
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Figure 1: Comparison of the conceptual amino acid sequences of nocturnin homologues from chicken (CNOC), cow (BNOC), rat, (RNOC), Xenopus (XNOC), mouse (MNOC), human (hNoc) and Drosophila (DNOC). Sequences were analyzed using the Clustal W Alignment procedure. Gaps indicated with dots are inserted to achieve optimum alignment. Dark gray and light gray highlights indicate amino acid identities and similarities respectively. The horizontal bar marks the position of the heptad leucine repeat in Xenopus and the asterisks indicate the position of the leucines. The positions of introns 1 and 2 based on the Xenopus gene [36] are indicated by arrows. Note that both mouse [39] and human (from data in public databases of the National Library of Medicine) appear to have a similar gene structure based on 3 exons. The XNOC sequence is from GenBank accession number U74761, HNOC is from NP036250.1 [39], and DNOC is from AAF54601.1 [40]. Our complete mNoc cDNA from retina (accession number AF199491) has the same coding sequence as that reported earlier from liver [38,39]. The cNoc (AF199498), bNoc (AF199497), and rNoc (AF199495) partial sequences are from PCR amplified DNA segments.

Mentions: Blast analysis of public databases reveals a large number of coding sequences with significant similarity to Xenopus nocturnin (xNoc). As we originally reported [36], XNOC protein is similar to a large, C-terminal domain of the transcriptional regulator, CCR4, but appears to be lacking in several regulatory domains critical to CCR4 function. Recently, however, mouse and human cDNAs encoding homologues (Fig. 1A) of the same domain of CCR4, but comparable in size to XNOC [38,39] have been reported (Accession number # AAD56547 and AAD56548). Furthermore, availability of the complete genome ofDrosophila melanogaster [40] has revealed a coding sequence (AAF54601.1) with significant similarity to XNOC. In addition to these, we have recently added a complete coding sequence of mouse nocturnin cDNA from retina (mNoc) along with partial coding sequences for human (hNoc), bovine (bNoc), rat (rNoc) and chicken (cNoc).


Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse.

Wang Y, Osterbur DL, Megaw PL, Tosini G, Fukuhara C, Green CB, Besharse JC - BMC Dev. Biol. (2001)

Comparison of the conceptual amino acid sequences of nocturnin homologues from chicken (CNOC), cow (BNOC), rat, (RNOC), Xenopus (XNOC), mouse (MNOC), human (hNoc) and Drosophila (DNOC). Sequences were analyzed using the Clustal W Alignment procedure. Gaps indicated with dots are inserted to achieve optimum alignment. Dark gray and light gray highlights indicate amino acid identities and similarities respectively. The horizontal bar marks the position of the heptad leucine repeat in Xenopus and the asterisks indicate the position of the leucines. The positions of introns 1 and 2 based on the Xenopus gene [36] are indicated by arrows. Note that both mouse [39] and human (from data in public databases of the National Library of Medicine) appear to have a similar gene structure based on 3 exons. The XNOC sequence is from GenBank accession number U74761, HNOC is from NP036250.1 [39], and DNOC is from AAF54601.1 [40]. Our complete mNoc cDNA from retina (accession number AF199491) has the same coding sequence as that reported earlier from liver [38,39]. The cNoc (AF199498), bNoc (AF199497), and rNoc (AF199495) partial sequences are from PCR amplified DNA segments.
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Related In: Results  -  Collection

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Figure 1: Comparison of the conceptual amino acid sequences of nocturnin homologues from chicken (CNOC), cow (BNOC), rat, (RNOC), Xenopus (XNOC), mouse (MNOC), human (hNoc) and Drosophila (DNOC). Sequences were analyzed using the Clustal W Alignment procedure. Gaps indicated with dots are inserted to achieve optimum alignment. Dark gray and light gray highlights indicate amino acid identities and similarities respectively. The horizontal bar marks the position of the heptad leucine repeat in Xenopus and the asterisks indicate the position of the leucines. The positions of introns 1 and 2 based on the Xenopus gene [36] are indicated by arrows. Note that both mouse [39] and human (from data in public databases of the National Library of Medicine) appear to have a similar gene structure based on 3 exons. The XNOC sequence is from GenBank accession number U74761, HNOC is from NP036250.1 [39], and DNOC is from AAF54601.1 [40]. Our complete mNoc cDNA from retina (accession number AF199491) has the same coding sequence as that reported earlier from liver [38,39]. The cNoc (AF199498), bNoc (AF199497), and rNoc (AF199495) partial sequences are from PCR amplified DNA segments.
Mentions: Blast analysis of public databases reveals a large number of coding sequences with significant similarity to Xenopus nocturnin (xNoc). As we originally reported [36], XNOC protein is similar to a large, C-terminal domain of the transcriptional regulator, CCR4, but appears to be lacking in several regulatory domains critical to CCR4 function. Recently, however, mouse and human cDNAs encoding homologues (Fig. 1A) of the same domain of CCR4, but comparable in size to XNOC [38,39] have been reported (Accession number # AAD56547 and AAD56548). Furthermore, availability of the complete genome ofDrosophila melanogaster [40] has revealed a coding sequence (AAF54601.1) with significant similarity to XNOC. In addition to these, we have recently added a complete coding sequence of mouse nocturnin cDNA from retina (mNoc) along with partial coding sequences for human (hNoc), bovine (bNoc), rat (rNoc) and chicken (cNoc).

Bottom Line: Nocturnin was originally identified by differential display as a circadian clock regulated gene with high expression at night in photoreceptors of the African clawed frog, Xenopus laevis.Furthermore, mNoc exhibits circadian rhythmicity of mRNA abundance with peak levels at the time of light offset in the retina, spleen, heart, kidney and liver.The widespread expression and rhythmicity of mNoc mRNA parallels the widespread expression of other circadian clock genes in mammalian tissues, and suggests that nocturnin plays an important role in clock function or as a circadian clock effector.

View Article: PubMed Central - HTML - PubMed

Affiliation: PEL-FREEZ Clinical Systems, LLC, 9099 North Deerbrook Trail, Brown Deer, WI 53223, USA. ywang@pel-freez.com

ABSTRACT

Background: Nocturnin was originally identified by differential display as a circadian clock regulated gene with high expression at night in photoreceptors of the African clawed frog, Xenopus laevis. Although encoding a novel protein, the nocturnin cDNA had strong sequence similarity with a C-terminal domain of the yeast transcription factor CCR4, and with mouse and human ESTs. Since its original identification others have cloned mouse and human homologues of nocturnin/CCR4, and we have cloned a full-length cDNA from mouse retina, along with partial cDNAs from human, cow and chicken. The goal of this study was to determine the temporal pattern of nocturnin mRNA expression in multiple tissues of the mouse.

Results: cDNA sequence analysis revealed a high degree of conservation among vertebrate nocturnin/CCR4 homologues along with a possible homologue in Drosophila. Northern analysis of mRNA in C3H/He and C57/Bl6 mice revealed that the mNoc gene is expressed in a broad range of tissues, with greatest abundance in liver, kidney and testis. mNoc is also expressed in multiple brain regions including suprachiasmatic nucleus and pineal gland. Furthermore, mNoc exhibits circadian rhythmicity of mRNA abundance with peak levels at the time of light offset in the retina, spleen, heart, kidney and liver.

Conclusion: The widespread expression and rhythmicity of mNoc mRNA parallels the widespread expression of other circadian clock genes in mammalian tissues, and suggests that nocturnin plays an important role in clock function or as a circadian clock effector.

Show MeSH
Related in: MedlinePlus