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A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA.

Yu-Hong W, Rui C, Ding L - Nanoscale Res Lett (2011)

Bottom Line: The statistic analysis suggests that there is no significant difference between these two methods.Furthermore, this method is much quicker and easier than the type-specific PCR method.The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center of Biological Diagnosis and Therapy, No, 261 Hospital of PLA, Beijing 100094, China. liding261@163.com.

ABSTRACT

Background: The recent advance in nanomaterial research field prompts the development of diagnostics of infectious diseases greatly. Many nanomaterials have been developed and applied to molecular diagnostics in labs. At present, the diagnostic test of human papillomavirus (HPV) relies exclusively on molecular test. Hereon, we report a rapid and facile quantum dots (QDs) and superparamagnetic nanoparticle-based hybridization assay for the detection of (HPV) 16 infections which combines the merits of superparamagnetic nanoparticles and QDs and wholly differs from a conventional hybridization assay at that the reaction occurs at homogeneous solution, and total time for detection is no more than 1 h.

Methods: The probes were labeled with superparamagnetic nanoparticles and QDs. Sixty cervical swab samples were used to perform a hybridization assay with these probes, and the results were compared with type-specific polymerase chain reaction (PCR) method.

Results: The statistic analysis suggests that there is no significant difference between these two methods. Furthermore, this method is much quicker and easier than the type-specific PCR method.

Conclusion: This study has successfully validated the clinical performance of our hybridization assay. The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

No MeSH data available.


Related in: MedlinePlus

TEM characterization of superparamagnetic Fe3O4 nanoparticles.
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Figure 6: TEM characterization of superparamagnetic Fe3O4 nanoparticles.

Mentions: To demonstrate the formation of superparamagnetic nanoparticles, the as-prepared Fe3O4 solution was dropped on the copper grid coated with carbon film and characterized by transmission electron microscopy (JEOL, Tokyo, Japan. As seen in Figure 6, the size of Fe3O4 nanoparticles is about 20 nm. The power XRD pattern also shows that the as-prepared magnetite nanoclusters have an inverse spinel type structure (Figure 4B). The position and relative intensity of most peaks match well with standard Fe3O4 powder diffraction data (JCPDS89-0688), indicating that the magnetite nanocrystals in nanoclusters are crystalline. In addition, the nanoparticles could be enriched in 2 min by a NdFeB magnet, which means they have good magnetic property. After the removal of external magnetic field, these particles could be easily dispersed, suggesting their paramagnetism. The vibrating sample magnetometer (VSM) results of as-synthesized superparamagnetic nanoparticles indicate that they exhibit superparamagnetic behavior with a saturation moment of about 42.5 emu/g at 300 K, as shown in Figure 7. The SDS-PAGE results under silver staining indicate that probes have been conjugated to superparamagnetic nanoparticles (Figure 5B).


A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA.

Yu-Hong W, Rui C, Ding L - Nanoscale Res Lett (2011)

TEM characterization of superparamagnetic Fe3O4 nanoparticles.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211882&req=5

Figure 6: TEM characterization of superparamagnetic Fe3O4 nanoparticles.
Mentions: To demonstrate the formation of superparamagnetic nanoparticles, the as-prepared Fe3O4 solution was dropped on the copper grid coated with carbon film and characterized by transmission electron microscopy (JEOL, Tokyo, Japan. As seen in Figure 6, the size of Fe3O4 nanoparticles is about 20 nm. The power XRD pattern also shows that the as-prepared magnetite nanoclusters have an inverse spinel type structure (Figure 4B). The position and relative intensity of most peaks match well with standard Fe3O4 powder diffraction data (JCPDS89-0688), indicating that the magnetite nanocrystals in nanoclusters are crystalline. In addition, the nanoparticles could be enriched in 2 min by a NdFeB magnet, which means they have good magnetic property. After the removal of external magnetic field, these particles could be easily dispersed, suggesting their paramagnetism. The vibrating sample magnetometer (VSM) results of as-synthesized superparamagnetic nanoparticles indicate that they exhibit superparamagnetic behavior with a saturation moment of about 42.5 emu/g at 300 K, as shown in Figure 7. The SDS-PAGE results under silver staining indicate that probes have been conjugated to superparamagnetic nanoparticles (Figure 5B).

Bottom Line: The statistic analysis suggests that there is no significant difference between these two methods.Furthermore, this method is much quicker and easier than the type-specific PCR method.The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center of Biological Diagnosis and Therapy, No, 261 Hospital of PLA, Beijing 100094, China. liding261@163.com.

ABSTRACT

Background: The recent advance in nanomaterial research field prompts the development of diagnostics of infectious diseases greatly. Many nanomaterials have been developed and applied to molecular diagnostics in labs. At present, the diagnostic test of human papillomavirus (HPV) relies exclusively on molecular test. Hereon, we report a rapid and facile quantum dots (QDs) and superparamagnetic nanoparticle-based hybridization assay for the detection of (HPV) 16 infections which combines the merits of superparamagnetic nanoparticles and QDs and wholly differs from a conventional hybridization assay at that the reaction occurs at homogeneous solution, and total time for detection is no more than 1 h.

Methods: The probes were labeled with superparamagnetic nanoparticles and QDs. Sixty cervical swab samples were used to perform a hybridization assay with these probes, and the results were compared with type-specific polymerase chain reaction (PCR) method.

Results: The statistic analysis suggests that there is no significant difference between these two methods. Furthermore, this method is much quicker and easier than the type-specific PCR method.

Conclusion: This study has successfully validated the clinical performance of our hybridization assay. The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

No MeSH data available.


Related in: MedlinePlus