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A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA.

Yu-Hong W, Rui C, Ding L - Nanoscale Res Lett (2011)

Bottom Line: The statistic analysis suggests that there is no significant difference between these two methods.Furthermore, this method is much quicker and easier than the type-specific PCR method.The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center of Biological Diagnosis and Therapy, No, 261 Hospital of PLA, Beijing 100094, China. liding261@163.com.

ABSTRACT

Background: The recent advance in nanomaterial research field prompts the development of diagnostics of infectious diseases greatly. Many nanomaterials have been developed and applied to molecular diagnostics in labs. At present, the diagnostic test of human papillomavirus (HPV) relies exclusively on molecular test. Hereon, we report a rapid and facile quantum dots (QDs) and superparamagnetic nanoparticle-based hybridization assay for the detection of (HPV) 16 infections which combines the merits of superparamagnetic nanoparticles and QDs and wholly differs from a conventional hybridization assay at that the reaction occurs at homogeneous solution, and total time for detection is no more than 1 h.

Methods: The probes were labeled with superparamagnetic nanoparticles and QDs. Sixty cervical swab samples were used to perform a hybridization assay with these probes, and the results were compared with type-specific polymerase chain reaction (PCR) method.

Results: The statistic analysis suggests that there is no significant difference between these two methods. Furthermore, this method is much quicker and easier than the type-specific PCR method.

Conclusion: This study has successfully validated the clinical performance of our hybridization assay. The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

No MeSH data available.


Related in: MedlinePlus

Fluorescent spectrum of QDs.
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Figure 3: Fluorescent spectrum of QDs.

Mentions: The as-prepared quantum dots are red solution. According to the absorbance spectrum and emission spectrum measured by UV spectrophotometer and luminescence spectrometer, they could be excited effectively under ultraviolet band, and their maximum emission peak is about 530 nm, which means the resultant quantum dots is fluorescence-active and could be used as a fluorescent probe (Figures 2, 3). The X-Ray diffraction analysis indicates that the as-prepared QDs exhibit a zinc blende cubic structure (Figure 4A). The position and relative intensity of most peaks match well with standard CdTe powder diffraction data (JCPDS82-0474). The SDS-PAGE results under UV lamp indicate that probes have been conjugated to QDs (Figure 5A).


A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA.

Yu-Hong W, Rui C, Ding L - Nanoscale Res Lett (2011)

Fluorescent spectrum of QDs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211882&req=5

Figure 3: Fluorescent spectrum of QDs.
Mentions: The as-prepared quantum dots are red solution. According to the absorbance spectrum and emission spectrum measured by UV spectrophotometer and luminescence spectrometer, they could be excited effectively under ultraviolet band, and their maximum emission peak is about 530 nm, which means the resultant quantum dots is fluorescence-active and could be used as a fluorescent probe (Figures 2, 3). The X-Ray diffraction analysis indicates that the as-prepared QDs exhibit a zinc blende cubic structure (Figure 4A). The position and relative intensity of most peaks match well with standard CdTe powder diffraction data (JCPDS82-0474). The SDS-PAGE results under UV lamp indicate that probes have been conjugated to QDs (Figure 5A).

Bottom Line: The statistic analysis suggests that there is no significant difference between these two methods.Furthermore, this method is much quicker and easier than the type-specific PCR method.The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center of Biological Diagnosis and Therapy, No, 261 Hospital of PLA, Beijing 100094, China. liding261@163.com.

ABSTRACT

Background: The recent advance in nanomaterial research field prompts the development of diagnostics of infectious diseases greatly. Many nanomaterials have been developed and applied to molecular diagnostics in labs. At present, the diagnostic test of human papillomavirus (HPV) relies exclusively on molecular test. Hereon, we report a rapid and facile quantum dots (QDs) and superparamagnetic nanoparticle-based hybridization assay for the detection of (HPV) 16 infections which combines the merits of superparamagnetic nanoparticles and QDs and wholly differs from a conventional hybridization assay at that the reaction occurs at homogeneous solution, and total time for detection is no more than 1 h.

Methods: The probes were labeled with superparamagnetic nanoparticles and QDs. Sixty cervical swab samples were used to perform a hybridization assay with these probes, and the results were compared with type-specific polymerase chain reaction (PCR) method.

Results: The statistic analysis suggests that there is no significant difference between these two methods. Furthermore, this method is much quicker and easier than the type-specific PCR method.

Conclusion: This study has successfully validated the clinical performance of our hybridization assay. The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

No MeSH data available.


Related in: MedlinePlus