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A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA.

Yu-Hong W, Rui C, Ding L - Nanoscale Res Lett (2011)

Bottom Line: The statistic analysis suggests that there is no significant difference between these two methods.Furthermore, this method is much quicker and easier than the type-specific PCR method.The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center of Biological Diagnosis and Therapy, No, 261 Hospital of PLA, Beijing 100094, China. liding261@163.com.

ABSTRACT

Background: The recent advance in nanomaterial research field prompts the development of diagnostics of infectious diseases greatly. Many nanomaterials have been developed and applied to molecular diagnostics in labs. At present, the diagnostic test of human papillomavirus (HPV) relies exclusively on molecular test. Hereon, we report a rapid and facile quantum dots (QDs) and superparamagnetic nanoparticle-based hybridization assay for the detection of (HPV) 16 infections which combines the merits of superparamagnetic nanoparticles and QDs and wholly differs from a conventional hybridization assay at that the reaction occurs at homogeneous solution, and total time for detection is no more than 1 h.

Methods: The probes were labeled with superparamagnetic nanoparticles and QDs. Sixty cervical swab samples were used to perform a hybridization assay with these probes, and the results were compared with type-specific polymerase chain reaction (PCR) method.

Results: The statistic analysis suggests that there is no significant difference between these two methods. Furthermore, this method is much quicker and easier than the type-specific PCR method.

Conclusion: This study has successfully validated the clinical performance of our hybridization assay. The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

No MeSH data available.


Related in: MedlinePlus

The rationale of QDs and superparamagnetic nanoparticle-based hybridization.
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Figure 1: The rationale of QDs and superparamagnetic nanoparticle-based hybridization.

Mentions: Ten HPV-16-negative human DNA samples were used to determine the cutoff value of QDs and superparamagnetic nanoparticle-based hybridization. The detection procedure was described in detail in the next section (Figure 1). The cutoff value was defined as the mean fluorescence intensity of HPV-16-negative human DNA samples minus double standard deviations (CV). A result under cutoff value in succedent detection was determined as a positive result. The ten HPV-16-positive samples were used to validate our hybridization assay on the basis of the cutoff value.


A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA.

Yu-Hong W, Rui C, Ding L - Nanoscale Res Lett (2011)

The rationale of QDs and superparamagnetic nanoparticle-based hybridization.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211882&req=5

Figure 1: The rationale of QDs and superparamagnetic nanoparticle-based hybridization.
Mentions: Ten HPV-16-negative human DNA samples were used to determine the cutoff value of QDs and superparamagnetic nanoparticle-based hybridization. The detection procedure was described in detail in the next section (Figure 1). The cutoff value was defined as the mean fluorescence intensity of HPV-16-negative human DNA samples minus double standard deviations (CV). A result under cutoff value in succedent detection was determined as a positive result. The ten HPV-16-positive samples were used to validate our hybridization assay on the basis of the cutoff value.

Bottom Line: The statistic analysis suggests that there is no significant difference between these two methods.Furthermore, this method is much quicker and easier than the type-specific PCR method.The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center of Biological Diagnosis and Therapy, No, 261 Hospital of PLA, Beijing 100094, China. liding261@163.com.

ABSTRACT

Background: The recent advance in nanomaterial research field prompts the development of diagnostics of infectious diseases greatly. Many nanomaterials have been developed and applied to molecular diagnostics in labs. At present, the diagnostic test of human papillomavirus (HPV) relies exclusively on molecular test. Hereon, we report a rapid and facile quantum dots (QDs) and superparamagnetic nanoparticle-based hybridization assay for the detection of (HPV) 16 infections which combines the merits of superparamagnetic nanoparticles and QDs and wholly differs from a conventional hybridization assay at that the reaction occurs at homogeneous solution, and total time for detection is no more than 1 h.

Methods: The probes were labeled with superparamagnetic nanoparticles and QDs. Sixty cervical swab samples were used to perform a hybridization assay with these probes, and the results were compared with type-specific polymerase chain reaction (PCR) method.

Results: The statistic analysis suggests that there is no significant difference between these two methods. Furthermore, this method is much quicker and easier than the type-specific PCR method.

Conclusion: This study has successfully validated the clinical performance of our hybridization assay. The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

No MeSH data available.


Related in: MedlinePlus