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PLGA-based gene delivering nanoparticle enhance suppression effect of miRNA in HePG2 cells.

Liang GF, Zhu YL, Sun B, Hu FH, Tian T, Li SC, Xiao ZD - Nanoscale Res Lett (2011)

Bottom Line: The N/P ratio (ratio of the polymer nitrogen to the DNA phosphate) 6 of the PLGA/PEI/DNA nanocomplex displays the best property among various N/P proportions, yielding similar transfection efficiency when compared to Lipofectamine/DNA lipoplexes.Moreover, nanocomplex shows better serum compatibility than commercial liposome.Therefore, these results demonstrate that PLGA/PEI nanoparticles are promising non-viral vectors for gene delivery.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. zdxiao@seu.edu.

ABSTRACT
The biggest challenge in the field of gene therapy is how to effectively deliver target genes to special cells. This study aimed to develop a new type of poly(D,L-lactide-co-glycolide) (PLGA)-based nanoparticles for gene delivery, which are capable of overcoming the disadvantages of polyethylenimine (PEI)- or cationic liposome-based gene carrier, such as the cytotoxicity induced by excess positive charge, as well as the aggregation on the cell surface. The PLGA-based nanoparticles presented in this study were synthesized by emulsion evaporation method and characterized by transmission electron microscopy, dynamic light scattering, and energy dispersive spectroscopy. The size of PLGA/PEI nanoparticles in phosphate-buffered saline (PBS) was about 60 nm at the optimal charge ratio. Without observable aggregation, the nanoparticles showed a better monodispersity. The PLGA-based nanoparticles were used as vector carrier for miRNA transfection in HepG2 cells. It exhibited a higher transfection efficiency and lower cytotoxicity in HepG2 cells compared to the PEI/DNA complex. The N/P ratio (ratio of the polymer nitrogen to the DNA phosphate) 6 of the PLGA/PEI/DNA nanocomplex displays the best property among various N/P proportions, yielding similar transfection efficiency when compared to Lipofectamine/DNA lipoplexes. Moreover, nanocomplex shows better serum compatibility than commercial liposome. PLGA nanocomplexes obviously accumulate in tumor cells after transfection, which indicate that the complexes contribute to cellular uptake of pDNA and pronouncedly enhance the treatment effect of miR-26a by inducing cell cycle arrest. Therefore, these results demonstrate that PLGA/PEI nanoparticles are promising non-viral vectors for gene delivery.

No MeSH data available.


Related in: MedlinePlus

TEM images of free PLGA nanoparticle; PLGA/PEI, and PLGA/PEI/pDNA. Scale bars: 100, 50, and 200 nm, respectively.
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Figure 1: TEM images of free PLGA nanoparticle; PLGA/PEI, and PLGA/PEI/pDNA. Scale bars: 100, 50, and 200 nm, respectively.

Mentions: In this study, PLGA-based nanoparticle was developed and characterized using TEM, DLS, and EDS. Figure 1 showed different characteristics of PLGA nanoparticles with PEI or PEI/pDNA. The diameter of the nanoparticles/DNA complexes ranged from 45 to 70 nm at the determined nanoparticles/DNA N/P ratios. Slight size changes were observed following coating of the biodegradable PLGA nanoparticles with PEI or PEI/pDNA. The mean diameter of the sole PLGA nanoparticles was approximately 50 nm, whereas the sizes of complexed PLGA nanoparticles containing PEI or PEI/pDNA are increased to about 57 or 60 nm, respectively. These results are consistent with our DLS observations and indicate that the PLGA nanoparticle size may be increased with PEI or polyplexed PEI/pDNA coating.


PLGA-based gene delivering nanoparticle enhance suppression effect of miRNA in HePG2 cells.

Liang GF, Zhu YL, Sun B, Hu FH, Tian T, Li SC, Xiao ZD - Nanoscale Res Lett (2011)

TEM images of free PLGA nanoparticle; PLGA/PEI, and PLGA/PEI/pDNA. Scale bars: 100, 50, and 200 nm, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211866&req=5

Figure 1: TEM images of free PLGA nanoparticle; PLGA/PEI, and PLGA/PEI/pDNA. Scale bars: 100, 50, and 200 nm, respectively.
Mentions: In this study, PLGA-based nanoparticle was developed and characterized using TEM, DLS, and EDS. Figure 1 showed different characteristics of PLGA nanoparticles with PEI or PEI/pDNA. The diameter of the nanoparticles/DNA complexes ranged from 45 to 70 nm at the determined nanoparticles/DNA N/P ratios. Slight size changes were observed following coating of the biodegradable PLGA nanoparticles with PEI or PEI/pDNA. The mean diameter of the sole PLGA nanoparticles was approximately 50 nm, whereas the sizes of complexed PLGA nanoparticles containing PEI or PEI/pDNA are increased to about 57 or 60 nm, respectively. These results are consistent with our DLS observations and indicate that the PLGA nanoparticle size may be increased with PEI or polyplexed PEI/pDNA coating.

Bottom Line: The N/P ratio (ratio of the polymer nitrogen to the DNA phosphate) 6 of the PLGA/PEI/DNA nanocomplex displays the best property among various N/P proportions, yielding similar transfection efficiency when compared to Lipofectamine/DNA lipoplexes.Moreover, nanocomplex shows better serum compatibility than commercial liposome.Therefore, these results demonstrate that PLGA/PEI nanoparticles are promising non-viral vectors for gene delivery.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. zdxiao@seu.edu.

ABSTRACT
The biggest challenge in the field of gene therapy is how to effectively deliver target genes to special cells. This study aimed to develop a new type of poly(D,L-lactide-co-glycolide) (PLGA)-based nanoparticles for gene delivery, which are capable of overcoming the disadvantages of polyethylenimine (PEI)- or cationic liposome-based gene carrier, such as the cytotoxicity induced by excess positive charge, as well as the aggregation on the cell surface. The PLGA-based nanoparticles presented in this study were synthesized by emulsion evaporation method and characterized by transmission electron microscopy, dynamic light scattering, and energy dispersive spectroscopy. The size of PLGA/PEI nanoparticles in phosphate-buffered saline (PBS) was about 60 nm at the optimal charge ratio. Without observable aggregation, the nanoparticles showed a better monodispersity. The PLGA-based nanoparticles were used as vector carrier for miRNA transfection in HepG2 cells. It exhibited a higher transfection efficiency and lower cytotoxicity in HepG2 cells compared to the PEI/DNA complex. The N/P ratio (ratio of the polymer nitrogen to the DNA phosphate) 6 of the PLGA/PEI/DNA nanocomplex displays the best property among various N/P proportions, yielding similar transfection efficiency when compared to Lipofectamine/DNA lipoplexes. Moreover, nanocomplex shows better serum compatibility than commercial liposome. PLGA nanocomplexes obviously accumulate in tumor cells after transfection, which indicate that the complexes contribute to cellular uptake of pDNA and pronouncedly enhance the treatment effect of miR-26a by inducing cell cycle arrest. Therefore, these results demonstrate that PLGA/PEI nanoparticles are promising non-viral vectors for gene delivery.

No MeSH data available.


Related in: MedlinePlus