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Spontaneous confocal Raman microscopy--a tool to study the uptake of nanoparticles and carbon nanotubes into cells.

Romero G, Rojas E, Estrela-Lopis I, Donath E, Moya SE - Nanoscale Res Lett (2011)

Bottom Line: Confocal Raman microscopy as a label-free technique was applied to study the uptake and internalization of poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) and carbon nanotubes (CNTs) into hepatocarcinoma human HepG2 cells.Spontaneous confocal Raman spectra was recorded from the cells exposed to oxidized CNTs and to PLGA NPs.For PLGA NPs, it was found that they preferentially co-localized with lipid bodies, while the oxidized CNTs are located in the cytoplasm.

View Article: PubMed Central - HTML - PubMed

Affiliation: CIC biomaGUNE, Paseo Miramón 182 C, 20009 San Sebastian, Spain. smoya@cicbiomagune.es.

ABSTRACT
Confocal Raman microscopy as a label-free technique was applied to study the uptake and internalization of poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) and carbon nanotubes (CNTs) into hepatocarcinoma human HepG2 cells. Spontaneous confocal Raman spectra was recorded from the cells exposed to oxidized CNTs and to PLGA NPs. The Raman spectra showed bands arising from the cellular environment: lipids, proteins, nucleic acids, as well as bands characteristic for either PLGA NPs or CNTs. The simultaneous generation of Raman bands from the cell and nanomaterials from the same spot proves internalization, and also indicates the cellular region, where the nanomaterial is located. For PLGA NPs, it was found that they preferentially co-localized with lipid bodies, while the oxidized CNTs are located in the cytoplasm.

No MeSH data available.


Related in: MedlinePlus

Characterization and confocal fluorescence imaging of PLGA NPs in cells. (a) TEM morphology of PLGA NPs stabilized with PEI and (b)CLSM images of HepG2 cells after being co-cultured with PLGA NPsstabilized with PEI.
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Figure 1: Characterization and confocal fluorescence imaging of PLGA NPs in cells. (a) TEM morphology of PLGA NPs stabilized with PEI and (b)CLSM images of HepG2 cells after being co-cultured with PLGA NPsstabilized with PEI.

Mentions: In Figure 1a, a characteristic TEM image of the PLGA NPs is shown--the size of the NPs ranges from approximately 250-400 nm. PLGA NPs were labelled with Rd6G for visualization in HepG2 cells with CLSM. In Figure 1b, the confocal images show that PLGA NPs are associated with the cells. This follows from the red colour indicating the Rd6G-labelled NPs, distributed around the blue-stained nucleus. The single confocal image does not unambiguously prove the internalization of the PLGA NPs in the cells, which could also be associated to the cell membrane. However, a scan in the z-direction (z-scan) could show the intracellular presence of the NPs, especially, if the plasma membrane had been also stained [9].


Spontaneous confocal Raman microscopy--a tool to study the uptake of nanoparticles and carbon nanotubes into cells.

Romero G, Rojas E, Estrela-Lopis I, Donath E, Moya SE - Nanoscale Res Lett (2011)

Characterization and confocal fluorescence imaging of PLGA NPs in cells. (a) TEM morphology of PLGA NPs stabilized with PEI and (b)CLSM images of HepG2 cells after being co-cultured with PLGA NPsstabilized with PEI.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211846&req=5

Figure 1: Characterization and confocal fluorescence imaging of PLGA NPs in cells. (a) TEM morphology of PLGA NPs stabilized with PEI and (b)CLSM images of HepG2 cells after being co-cultured with PLGA NPsstabilized with PEI.
Mentions: In Figure 1a, a characteristic TEM image of the PLGA NPs is shown--the size of the NPs ranges from approximately 250-400 nm. PLGA NPs were labelled with Rd6G for visualization in HepG2 cells with CLSM. In Figure 1b, the confocal images show that PLGA NPs are associated with the cells. This follows from the red colour indicating the Rd6G-labelled NPs, distributed around the blue-stained nucleus. The single confocal image does not unambiguously prove the internalization of the PLGA NPs in the cells, which could also be associated to the cell membrane. However, a scan in the z-direction (z-scan) could show the intracellular presence of the NPs, especially, if the plasma membrane had been also stained [9].

Bottom Line: Confocal Raman microscopy as a label-free technique was applied to study the uptake and internalization of poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) and carbon nanotubes (CNTs) into hepatocarcinoma human HepG2 cells.Spontaneous confocal Raman spectra was recorded from the cells exposed to oxidized CNTs and to PLGA NPs.For PLGA NPs, it was found that they preferentially co-localized with lipid bodies, while the oxidized CNTs are located in the cytoplasm.

View Article: PubMed Central - HTML - PubMed

Affiliation: CIC biomaGUNE, Paseo Miramón 182 C, 20009 San Sebastian, Spain. smoya@cicbiomagune.es.

ABSTRACT
Confocal Raman microscopy as a label-free technique was applied to study the uptake and internalization of poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) and carbon nanotubes (CNTs) into hepatocarcinoma human HepG2 cells. Spontaneous confocal Raman spectra was recorded from the cells exposed to oxidized CNTs and to PLGA NPs. The Raman spectra showed bands arising from the cellular environment: lipids, proteins, nucleic acids, as well as bands characteristic for either PLGA NPs or CNTs. The simultaneous generation of Raman bands from the cell and nanomaterials from the same spot proves internalization, and also indicates the cellular region, where the nanomaterial is located. For PLGA NPs, it was found that they preferentially co-localized with lipid bodies, while the oxidized CNTs are located in the cytoplasm.

No MeSH data available.


Related in: MedlinePlus