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CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation.

Zhang G, Shi L, Selke M, Wang X - Nanoscale Res Lett (2011)

Bottom Line: Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells.We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells.Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Department of Biological Science and Medical Engineering Southeast University, Nanjing, 210096, PR China. xuewang@seu.edu.cn.

ABSTRACT
Cadmium telluride quantum dots (Cdte QDs) have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery. In this study, we have investigated the interaction mechanism and synergistic effect of 3-mercaptopropionic acid-capped Cdte QDs with the anti-cancer drug daunorubicin (DNR) on the induction of apoptosis using drug-resistant human hepatoma HepG2/ADM cells. Electrochemical assay revealed that Cdte QDs readily facilitated the uptake of the DNR into HepG2/ADM cells. Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells. We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells. Moreover, our in vivo study indicated that the treatment of Cdte QDs together with DNR effectively inhibited the human hepatoma HepG2/ADM nude mice tumor growth. The increased cell apoptosis rate was closely correlated with the enhanced inhibition of tumor growth in the studied animals. Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

No MeSH data available.


Related in: MedlinePlus

DNA fragmentation in HepG2/ADM cells after different treatments. Genomic DNA was isolated from HepG2/ADM cells. DNA ladders were visualized under UV light with ethidium bromide staining. HepG2/ADM cells treated with: control treatment; 4 × 10-6 mol/L DNR; 4 μM Cdte QDs; and 4 μM Cdte QDs + 4 × 10-6 mol/L DNR for 72 h.
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Figure 5: DNA fragmentation in HepG2/ADM cells after different treatments. Genomic DNA was isolated from HepG2/ADM cells. DNA ladders were visualized under UV light with ethidium bromide staining. HepG2/ADM cells treated with: control treatment; 4 × 10-6 mol/L DNR; 4 μM Cdte QDs; and 4 μM Cdte QDs + 4 × 10-6 mol/L DNR for 72 h.

Mentions: The DNA fragmentations were examined. When HepG2/ADM cells were treated with Cdte QDs + DNR, the intensity of fragmented chromosomal DNA bands was much higher than that observed from cells treated with Cdte QDs, or DNR alone (Figure 5). These results provide evidence that the remarkable enhancement of apoptosis was induced by synergistic effects of Cdte QDs and DNR on HepG2/ADM cells.


CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation.

Zhang G, Shi L, Selke M, Wang X - Nanoscale Res Lett (2011)

DNA fragmentation in HepG2/ADM cells after different treatments. Genomic DNA was isolated from HepG2/ADM cells. DNA ladders were visualized under UV light with ethidium bromide staining. HepG2/ADM cells treated with: control treatment; 4 × 10-6 mol/L DNR; 4 μM Cdte QDs; and 4 μM Cdte QDs + 4 × 10-6 mol/L DNR for 72 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211514&req=5

Figure 5: DNA fragmentation in HepG2/ADM cells after different treatments. Genomic DNA was isolated from HepG2/ADM cells. DNA ladders were visualized under UV light with ethidium bromide staining. HepG2/ADM cells treated with: control treatment; 4 × 10-6 mol/L DNR; 4 μM Cdte QDs; and 4 μM Cdte QDs + 4 × 10-6 mol/L DNR for 72 h.
Mentions: The DNA fragmentations were examined. When HepG2/ADM cells were treated with Cdte QDs + DNR, the intensity of fragmented chromosomal DNA bands was much higher than that observed from cells treated with Cdte QDs, or DNR alone (Figure 5). These results provide evidence that the remarkable enhancement of apoptosis was induced by synergistic effects of Cdte QDs and DNR on HepG2/ADM cells.

Bottom Line: Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells.We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells.Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Department of Biological Science and Medical Engineering Southeast University, Nanjing, 210096, PR China. xuewang@seu.edu.cn.

ABSTRACT
Cadmium telluride quantum dots (Cdte QDs) have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery. In this study, we have investigated the interaction mechanism and synergistic effect of 3-mercaptopropionic acid-capped Cdte QDs with the anti-cancer drug daunorubicin (DNR) on the induction of apoptosis using drug-resistant human hepatoma HepG2/ADM cells. Electrochemical assay revealed that Cdte QDs readily facilitated the uptake of the DNR into HepG2/ADM cells. Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells. We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells. Moreover, our in vivo study indicated that the treatment of Cdte QDs together with DNR effectively inhibited the human hepatoma HepG2/ADM nude mice tumor growth. The increased cell apoptosis rate was closely correlated with the enhanced inhibition of tumor growth in the studied animals. Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

No MeSH data available.


Related in: MedlinePlus