Limits...
CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation.

Zhang G, Shi L, Selke M, Wang X - Nanoscale Res Lett (2011)

Bottom Line: Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells.We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells.Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Department of Biological Science and Medical Engineering Southeast University, Nanjing, 210096, PR China. xuewang@seu.edu.cn.

ABSTRACT
Cadmium telluride quantum dots (Cdte QDs) have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery. In this study, we have investigated the interaction mechanism and synergistic effect of 3-mercaptopropionic acid-capped Cdte QDs with the anti-cancer drug daunorubicin (DNR) on the induction of apoptosis using drug-resistant human hepatoma HepG2/ADM cells. Electrochemical assay revealed that Cdte QDs readily facilitated the uptake of the DNR into HepG2/ADM cells. Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells. We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells. Moreover, our in vivo study indicated that the treatment of Cdte QDs together with DNR effectively inhibited the human hepatoma HepG2/ADM nude mice tumor growth. The increased cell apoptosis rate was closely correlated with the enhanced inhibition of tumor growth in the studied animals. Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

No MeSH data available.


Related in: MedlinePlus

Assay of cell apoptosis rate and morphological images: (A) Detection of apoptotic and normal cells by acridine orange staining. Control cell nuclei, apoptotic nuclei from HepG2/ADM cells ware observed. (B) HepG2/ADM cells detected by flow cytometry using Annexin-V-FITC method. (a) control treatment; (b) 4 × 10-6 mol/L DNR treatment; (c) 4 μM Cdte QDs treatment; and (d) 4 μM Cdte QDs + 4 × 10-6 mol/L DNR for 36 h. (C) Quantitative analysis of apoptotic cells after various treatments shown in (B). *p < 0.05, compared to the control treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3211514&req=5

Figure 4: Assay of cell apoptosis rate and morphological images: (A) Detection of apoptotic and normal cells by acridine orange staining. Control cell nuclei, apoptotic nuclei from HepG2/ADM cells ware observed. (B) HepG2/ADM cells detected by flow cytometry using Annexin-V-FITC method. (a) control treatment; (b) 4 × 10-6 mol/L DNR treatment; (c) 4 μM Cdte QDs treatment; and (d) 4 μM Cdte QDs + 4 × 10-6 mol/L DNR for 36 h. (C) Quantitative analysis of apoptotic cells after various treatments shown in (B). *p < 0.05, compared to the control treatment.

Mentions: Using acridine orange/ethidium bromide (AO/EB) dye mixture staining for apoptotic cells, apoptotic nuclei were identified by their distinctively marginated and fragmented appearance under the fluorescence microscope. The apoptotic nuclei of HepG2/ADM cells (Figure 4A, apoptosis nuclei) at 72 h could be identified by their distinctively marginated and fragmented appearance. For the control cells without treatment, cells nuclei were normal as shown in (Figure 4A, control nuclei). Figure 4B shows that Annexin-V-FITC apoptosis detection, Cdte QDs + DNR induced a much higher HepG2/ADM cell apoptosis rate than that of DNR, Cdte QDs, or untreated control. We found that the percentage of apoptotic cells was 67.4%, 26.8%, 15.2%, 8.5% for the treatment with Cdte QDs + DNR, Cdte QDs, DNR, untreatment, respectively (Figure 4C).


CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation.

Zhang G, Shi L, Selke M, Wang X - Nanoscale Res Lett (2011)

Assay of cell apoptosis rate and morphological images: (A) Detection of apoptotic and normal cells by acridine orange staining. Control cell nuclei, apoptotic nuclei from HepG2/ADM cells ware observed. (B) HepG2/ADM cells detected by flow cytometry using Annexin-V-FITC method. (a) control treatment; (b) 4 × 10-6 mol/L DNR treatment; (c) 4 μM Cdte QDs treatment; and (d) 4 μM Cdte QDs + 4 × 10-6 mol/L DNR for 36 h. (C) Quantitative analysis of apoptotic cells after various treatments shown in (B). *p < 0.05, compared to the control treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211514&req=5

Figure 4: Assay of cell apoptosis rate and morphological images: (A) Detection of apoptotic and normal cells by acridine orange staining. Control cell nuclei, apoptotic nuclei from HepG2/ADM cells ware observed. (B) HepG2/ADM cells detected by flow cytometry using Annexin-V-FITC method. (a) control treatment; (b) 4 × 10-6 mol/L DNR treatment; (c) 4 μM Cdte QDs treatment; and (d) 4 μM Cdte QDs + 4 × 10-6 mol/L DNR for 36 h. (C) Quantitative analysis of apoptotic cells after various treatments shown in (B). *p < 0.05, compared to the control treatment.
Mentions: Using acridine orange/ethidium bromide (AO/EB) dye mixture staining for apoptotic cells, apoptotic nuclei were identified by their distinctively marginated and fragmented appearance under the fluorescence microscope. The apoptotic nuclei of HepG2/ADM cells (Figure 4A, apoptosis nuclei) at 72 h could be identified by their distinctively marginated and fragmented appearance. For the control cells without treatment, cells nuclei were normal as shown in (Figure 4A, control nuclei). Figure 4B shows that Annexin-V-FITC apoptosis detection, Cdte QDs + DNR induced a much higher HepG2/ADM cell apoptosis rate than that of DNR, Cdte QDs, or untreated control. We found that the percentage of apoptotic cells was 67.4%, 26.8%, 15.2%, 8.5% for the treatment with Cdte QDs + DNR, Cdte QDs, DNR, untreatment, respectively (Figure 4C).

Bottom Line: Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells.We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells.Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Department of Biological Science and Medical Engineering Southeast University, Nanjing, 210096, PR China. xuewang@seu.edu.cn.

ABSTRACT
Cadmium telluride quantum dots (Cdte QDs) have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery. In this study, we have investigated the interaction mechanism and synergistic effect of 3-mercaptopropionic acid-capped Cdte QDs with the anti-cancer drug daunorubicin (DNR) on the induction of apoptosis using drug-resistant human hepatoma HepG2/ADM cells. Electrochemical assay revealed that Cdte QDs readily facilitated the uptake of the DNR into HepG2/ADM cells. Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells. We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells. Moreover, our in vivo study indicated that the treatment of Cdte QDs together with DNR effectively inhibited the human hepatoma HepG2/ADM nude mice tumor growth. The increased cell apoptosis rate was closely correlated with the enhanced inhibition of tumor growth in the studied animals. Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

No MeSH data available.


Related in: MedlinePlus