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CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation.

Zhang G, Shi L, Selke M, Wang X - Nanoscale Res Lett (2011)

Bottom Line: Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells.We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells.Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Department of Biological Science and Medical Engineering Southeast University, Nanjing, 210096, PR China. xuewang@seu.edu.cn.

ABSTRACT
Cadmium telluride quantum dots (Cdte QDs) have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery. In this study, we have investigated the interaction mechanism and synergistic effect of 3-mercaptopropionic acid-capped Cdte QDs with the anti-cancer drug daunorubicin (DNR) on the induction of apoptosis using drug-resistant human hepatoma HepG2/ADM cells. Electrochemical assay revealed that Cdte QDs readily facilitated the uptake of the DNR into HepG2/ADM cells. Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells. We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells. Moreover, our in vivo study indicated that the treatment of Cdte QDs together with DNR effectively inhibited the human hepatoma HepG2/ADM nude mice tumor growth. The increased cell apoptosis rate was closely correlated with the enhanced inhibition of tumor growth in the studied animals. Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

No MeSH data available.


Related in: MedlinePlus

MTT assay of the growth inhibition rate of HepG2/ADM cells after various cellular treatments. The HepG2/ADM cells were treated with 1 × 10-6, 4 × 10-6, 16 × 10-6, 64 × 10-6, 12.8 × 10-5, and 51.2 × 10-5 mol/L of DNR; 1, 2.5, 5, 10, 20, and 40 μM Cdte QDs; or 4 μM Cdte QDs with 1 × 10-6, 4 × 10-6, 16 × 10-6, 64 × 10-6, 12.8 × 10-5, and 51.2 × 10-5 mol/L of DNR, respectively. *p < 0.05, indicates the significant difference in comparison to no treatment.
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Figure 2: MTT assay of the growth inhibition rate of HepG2/ADM cells after various cellular treatments. The HepG2/ADM cells were treated with 1 × 10-6, 4 × 10-6, 16 × 10-6, 64 × 10-6, 12.8 × 10-5, and 51.2 × 10-5 mol/L of DNR; 1, 2.5, 5, 10, 20, and 40 μM Cdte QDs; or 4 μM Cdte QDs with 1 × 10-6, 4 × 10-6, 16 × 10-6, 64 × 10-6, 12.8 × 10-5, and 51.2 × 10-5 mol/L of DNR, respectively. *p < 0.05, indicates the significant difference in comparison to no treatment.

Mentions: The MTT assay was carried out to explore the relative inhibition for the proliferation of the cells. The cells were treated with different concentrations of DNR or Cdte QDs, or treated by different concentrations of DNR combined with Cdte QDs for 36 h. Since HepG2/ADM cells are drug-resistant cell line, the high-concentration DNR treatment only causes low growth inhibition for HepG2/ADM cells (as shown in Figure 2). However, the growth inhibition rate was significantly increased when HepG2/ADM cells were treated by DNR combined with Cdte QDs. Therefore, it is evident that the significant enhancement of the cell proliferation inhibition may be facilitated due to a synergistic effect of Cdte QDs with DNR to the drug-resistant HepG2/ADM cells.


CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation.

Zhang G, Shi L, Selke M, Wang X - Nanoscale Res Lett (2011)

MTT assay of the growth inhibition rate of HepG2/ADM cells after various cellular treatments. The HepG2/ADM cells were treated with 1 × 10-6, 4 × 10-6, 16 × 10-6, 64 × 10-6, 12.8 × 10-5, and 51.2 × 10-5 mol/L of DNR; 1, 2.5, 5, 10, 20, and 40 μM Cdte QDs; or 4 μM Cdte QDs with 1 × 10-6, 4 × 10-6, 16 × 10-6, 64 × 10-6, 12.8 × 10-5, and 51.2 × 10-5 mol/L of DNR, respectively. *p < 0.05, indicates the significant difference in comparison to no treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211514&req=5

Figure 2: MTT assay of the growth inhibition rate of HepG2/ADM cells after various cellular treatments. The HepG2/ADM cells were treated with 1 × 10-6, 4 × 10-6, 16 × 10-6, 64 × 10-6, 12.8 × 10-5, and 51.2 × 10-5 mol/L of DNR; 1, 2.5, 5, 10, 20, and 40 μM Cdte QDs; or 4 μM Cdte QDs with 1 × 10-6, 4 × 10-6, 16 × 10-6, 64 × 10-6, 12.8 × 10-5, and 51.2 × 10-5 mol/L of DNR, respectively. *p < 0.05, indicates the significant difference in comparison to no treatment.
Mentions: The MTT assay was carried out to explore the relative inhibition for the proliferation of the cells. The cells were treated with different concentrations of DNR or Cdte QDs, or treated by different concentrations of DNR combined with Cdte QDs for 36 h. Since HepG2/ADM cells are drug-resistant cell line, the high-concentration DNR treatment only causes low growth inhibition for HepG2/ADM cells (as shown in Figure 2). However, the growth inhibition rate was significantly increased when HepG2/ADM cells were treated by DNR combined with Cdte QDs. Therefore, it is evident that the significant enhancement of the cell proliferation inhibition may be facilitated due to a synergistic effect of Cdte QDs with DNR to the drug-resistant HepG2/ADM cells.

Bottom Line: Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells.We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells.Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Department of Biological Science and Medical Engineering Southeast University, Nanjing, 210096, PR China. xuewang@seu.edu.cn.

ABSTRACT
Cadmium telluride quantum dots (Cdte QDs) have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery. In this study, we have investigated the interaction mechanism and synergistic effect of 3-mercaptopropionic acid-capped Cdte QDs with the anti-cancer drug daunorubicin (DNR) on the induction of apoptosis using drug-resistant human hepatoma HepG2/ADM cells. Electrochemical assay revealed that Cdte QDs readily facilitated the uptake of the DNR into HepG2/ADM cells. Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells. We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells. Moreover, our in vivo study indicated that the treatment of Cdte QDs together with DNR effectively inhibited the human hepatoma HepG2/ADM nude mice tumor growth. The increased cell apoptosis rate was closely correlated with the enhanced inhibition of tumor growth in the studied animals. Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

No MeSH data available.


Related in: MedlinePlus