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Peptide immobilisation on porous silicon surface for metal ions detection.

Sam SS, Chazalviel JN, Gouget-Laemmel AC, Ozanam FF, Etcheberry AA, Gabouze NE - Nanoscale Res Lett (2011)

Bottom Line: The recorded cyclic voltammograms show a quasi-irreversible process corresponding to the Cu(II)/Cu(I) couple.The kinetic factors (the heterogeneous rate constant and the transfer coefficient) and the stability constant of the complex formed on the porous silicon surface are determined.These results demonstrate the potential role of peptides grafted on porous silicon in developing strategies for simple and fast detection of metal ions in solution.

View Article: PubMed Central - HTML - PubMed

Affiliation: UDTS, 2 bd Frantz Fanon, BP 140, Alger-7 Merveilles, Algiers, Algeria. sabrina.sam@polytechnique.edu.

ABSTRACT
In this work, a Glycyl-Histidyl-Glycyl-Histidine (GlyHisGlyHis) peptide is covalently anchored to the porous silicon PSi surface using a multi-step reaction scheme compatible with the mild conditions required for preserving the probe activity. In a first step, alkene precursors are grafted onto the hydrogenated PSi surface using the hydrosilylation route, allowing for the formation of a carboxyl-terminated monolayer which is activated by reaction with N-hydroxysuccinimide in the presence of a peptide-coupling carbodiimide N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide and subsequently reacted with the amino linker of the peptide to form a covalent amide bond. Infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy are used to investigate the different steps of functionalization.The property of peptides to form stable complexes with metal ions is exploited to achieve metal-ion recognition by the peptide-modified PSi-based biosensor. An electrochemical study of the GlyHisGlyHis-modified PSi electrode is achieved in the presence of copper ions. The recorded cyclic voltammograms show a quasi-irreversible process corresponding to the Cu(II)/Cu(I) couple. The kinetic factors (the heterogeneous rate constant and the transfer coefficient) and the stability constant of the complex formed on the porous silicon surface are determined. These results demonstrate the potential role of peptides grafted on porous silicon in developing strategies for simple and fast detection of metal ions in solution.

No MeSH data available.


Related in: MedlinePlus

Reaction scheme of the transition metal complexation on a porous silicon sensor modified with peptide. In this case, Gly-His-Gly-His chelating Cu(II) cations.
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Figure 3: Reaction scheme of the transition metal complexation on a porous silicon sensor modified with peptide. In this case, Gly-His-Gly-His chelating Cu(II) cations.

Mentions: Figure 3 shows the reaction scheme of copper complexation on the GlyHisGlyHis-modified PSi during the accumulation step. The GlyHisGlyHis-modified PSi electrode is electrochemically inactive (Figure 4a) in the absence of copper (II). After copper accumulation for 15 min in a 0.1 mM Cu2+ solution and washing, the voltammogram recorded in a buffer solution that was free of copper exhibits cathodic and anodic peaks attributed to the quasi-reversible process of the Cu(I)/Cu(II) couple of copper chelated by the GlyHisGlyHis peptide immobilised on the PSi surface (Figure 4b).


Peptide immobilisation on porous silicon surface for metal ions detection.

Sam SS, Chazalviel JN, Gouget-Laemmel AC, Ozanam FF, Etcheberry AA, Gabouze NE - Nanoscale Res Lett (2011)

Reaction scheme of the transition metal complexation on a porous silicon sensor modified with peptide. In this case, Gly-His-Gly-His chelating Cu(II) cations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211508&req=5

Figure 3: Reaction scheme of the transition metal complexation on a porous silicon sensor modified with peptide. In this case, Gly-His-Gly-His chelating Cu(II) cations.
Mentions: Figure 3 shows the reaction scheme of copper complexation on the GlyHisGlyHis-modified PSi during the accumulation step. The GlyHisGlyHis-modified PSi electrode is electrochemically inactive (Figure 4a) in the absence of copper (II). After copper accumulation for 15 min in a 0.1 mM Cu2+ solution and washing, the voltammogram recorded in a buffer solution that was free of copper exhibits cathodic and anodic peaks attributed to the quasi-reversible process of the Cu(I)/Cu(II) couple of copper chelated by the GlyHisGlyHis peptide immobilised on the PSi surface (Figure 4b).

Bottom Line: The recorded cyclic voltammograms show a quasi-irreversible process corresponding to the Cu(II)/Cu(I) couple.The kinetic factors (the heterogeneous rate constant and the transfer coefficient) and the stability constant of the complex formed on the porous silicon surface are determined.These results demonstrate the potential role of peptides grafted on porous silicon in developing strategies for simple and fast detection of metal ions in solution.

View Article: PubMed Central - HTML - PubMed

Affiliation: UDTS, 2 bd Frantz Fanon, BP 140, Alger-7 Merveilles, Algiers, Algeria. sabrina.sam@polytechnique.edu.

ABSTRACT
In this work, a Glycyl-Histidyl-Glycyl-Histidine (GlyHisGlyHis) peptide is covalently anchored to the porous silicon PSi surface using a multi-step reaction scheme compatible with the mild conditions required for preserving the probe activity. In a first step, alkene precursors are grafted onto the hydrogenated PSi surface using the hydrosilylation route, allowing for the formation of a carboxyl-terminated monolayer which is activated by reaction with N-hydroxysuccinimide in the presence of a peptide-coupling carbodiimide N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide and subsequently reacted with the amino linker of the peptide to form a covalent amide bond. Infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy are used to investigate the different steps of functionalization.The property of peptides to form stable complexes with metal ions is exploited to achieve metal-ion recognition by the peptide-modified PSi-based biosensor. An electrochemical study of the GlyHisGlyHis-modified PSi electrode is achieved in the presence of copper ions. The recorded cyclic voltammograms show a quasi-irreversible process corresponding to the Cu(II)/Cu(I) couple. The kinetic factors (the heterogeneous rate constant and the transfer coefficient) and the stability constant of the complex formed on the porous silicon surface are determined. These results demonstrate the potential role of peptides grafted on porous silicon in developing strategies for simple and fast detection of metal ions in solution.

No MeSH data available.


Related in: MedlinePlus