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Surface properties of glass micropipettes and their effect on biological studies.

Malboubi M, Gu Y, Jiang K - Nanoscale Res Lett (2011)

Bottom Line: It is found that surface roughness parameters are strongly related on the tip size.The results of the experiments show that polished pipettes make significantly better seals.The results of this work are of important reference value for achieving pipettes with desired surface properties and can be used to explain biological phenomenon such as giga-seal formation.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Mechanical Engineering, The University of Birmingham, Edgbaston, Birmingham, B15 2TT UK. mlb@contacts.bham.ac.uk.

ABSTRACT
In this paper, an investigation on surface properties of glass micropipettes and their effect on biological applications is reported. Pipettes were pulled under different pulling conditions and the effect of each pulling parameter was analyzed. SEM stereoscopic technique was used to reveal the surface roughness properties of pipette tip and pipette inner wall in 3D. More than 20 pipettes were reconstructed. Pipette heads were split open using focused ion beam (FIB) milling for access to the inner walls. It is found that surface roughness parameters are strongly related on the tip size. Bigger pipettes have higher average surface roughness and lower developed interfacial area ratio. Furthermore, the autocorrelation of roughness model of the inner surface shows that the inner surface does not have any tendency of orientation and is not affected by pulling direction. To investigate the effect of surface roughness properties on biological applications, patch-clamping tests were carried out by conventional and FIB-polished pipettes. The results of the experiments show that polished pipettes make significantly better seals. The results of this work are of important reference value for achieving pipettes with desired surface properties and can be used to explain biological phenomenon such as giga-seal formation.

No MeSH data available.


Related in: MedlinePlus

The effect of pressure on tip diameter and average surface roughness. This control sets the pressure generated by the air compressor during the active cooling phase of the pull cycle. The unit of pressure is psi. Changes of less than 10 units will not be noticeable. Pressure is another way of controlling tip length and does not change the size of pipette tip significantly.
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Figure 7: The effect of pressure on tip diameter and average surface roughness. This control sets the pressure generated by the air compressor during the active cooling phase of the pull cycle. The unit of pressure is psi. Changes of less than 10 units will not be noticeable. Pressure is another way of controlling tip length and does not change the size of pipette tip significantly.

Mentions: Over 20 pipettes have been reconstructed in the study. The effect of each parameter is studied by investigating at least three reconstructions. Tip diameter (Dt) and average surface roughness (Sa) of all pipettes have been measured. Figures 3, 4, 5, 6 and 7 show correlations between pulling parameters and Dt and Sa.


Surface properties of glass micropipettes and their effect on biological studies.

Malboubi M, Gu Y, Jiang K - Nanoscale Res Lett (2011)

The effect of pressure on tip diameter and average surface roughness. This control sets the pressure generated by the air compressor during the active cooling phase of the pull cycle. The unit of pressure is psi. Changes of less than 10 units will not be noticeable. Pressure is another way of controlling tip length and does not change the size of pipette tip significantly.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211496&req=5

Figure 7: The effect of pressure on tip diameter and average surface roughness. This control sets the pressure generated by the air compressor during the active cooling phase of the pull cycle. The unit of pressure is psi. Changes of less than 10 units will not be noticeable. Pressure is another way of controlling tip length and does not change the size of pipette tip significantly.
Mentions: Over 20 pipettes have been reconstructed in the study. The effect of each parameter is studied by investigating at least three reconstructions. Tip diameter (Dt) and average surface roughness (Sa) of all pipettes have been measured. Figures 3, 4, 5, 6 and 7 show correlations between pulling parameters and Dt and Sa.

Bottom Line: It is found that surface roughness parameters are strongly related on the tip size.The results of the experiments show that polished pipettes make significantly better seals.The results of this work are of important reference value for achieving pipettes with desired surface properties and can be used to explain biological phenomenon such as giga-seal formation.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Mechanical Engineering, The University of Birmingham, Edgbaston, Birmingham, B15 2TT UK. mlb@contacts.bham.ac.uk.

ABSTRACT
In this paper, an investigation on surface properties of glass micropipettes and their effect on biological applications is reported. Pipettes were pulled under different pulling conditions and the effect of each pulling parameter was analyzed. SEM stereoscopic technique was used to reveal the surface roughness properties of pipette tip and pipette inner wall in 3D. More than 20 pipettes were reconstructed. Pipette heads were split open using focused ion beam (FIB) milling for access to the inner walls. It is found that surface roughness parameters are strongly related on the tip size. Bigger pipettes have higher average surface roughness and lower developed interfacial area ratio. Furthermore, the autocorrelation of roughness model of the inner surface shows that the inner surface does not have any tendency of orientation and is not affected by pulling direction. To investigate the effect of surface roughness properties on biological applications, patch-clamping tests were carried out by conventional and FIB-polished pipettes. The results of the experiments show that polished pipettes make significantly better seals. The results of this work are of important reference value for achieving pipettes with desired surface properties and can be used to explain biological phenomenon such as giga-seal formation.

No MeSH data available.


Related in: MedlinePlus