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Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue.

Bache M, Taboryski R, Schmid S, Aamand J, Jakobsen MH - Nanoscale Res Lett (2011)

Bottom Line: The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout.The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface.The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, 2800 Kongens Lyngby, Denmark. Michael.bache@nanotech.dtu.dk.

ABSTRACT
The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

No MeSH data available.


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Cantilever resonance frequency and bending. (Left) Mass/stiffness ratios of each of the four cantilevers divided in three groups: clean chip, after functionalization by micro-spotting, and after the addition of BAM antibody . The values are an average of eight experiments (104, 108, 112, 116, 117, 118, 119, and 120). (Right) Average bending values (μm) of cantilever tip relative to the chip body surface. Values are averages obtained from five experiments (116, 117, 118, 119, and 120).
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Figure 5: Cantilever resonance frequency and bending. (Left) Mass/stiffness ratios of each of the four cantilevers divided in three groups: clean chip, after functionalization by micro-spotting, and after the addition of BAM antibody . The values are an average of eight experiments (104, 108, 112, 116, 117, 118, 119, and 120). (Right) Average bending values (μm) of cantilever tip relative to the chip body surface. Values are averages obtained from five experiments (116, 117, 118, 119, and 120).

Mentions: The deflection values showed a clear bending of all cantilevers after the functionalization step (Figure 5, right). This was probably caused by salt deposits from the PBS buffer used in the micro-spotting of BAM-ovalbumine conjugate and ovalbumine. The cantilevers returned to their initial state after the experiment, probably caused by the removal of these deposited salts from the functionalization step. A large variation on the resonance frequency could explain the diverse signal variations obtained. The cantilevers showed a slight increase in variation of the resonance frequency after the functionalization step; but no significant difference could be seen after the experiment was performed (Figure 5, left).


Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue.

Bache M, Taboryski R, Schmid S, Aamand J, Jakobsen MH - Nanoscale Res Lett (2011)

Cantilever resonance frequency and bending. (Left) Mass/stiffness ratios of each of the four cantilevers divided in three groups: clean chip, after functionalization by micro-spotting, and after the addition of BAM antibody . The values are an average of eight experiments (104, 108, 112, 116, 117, 118, 119, and 120). (Right) Average bending values (μm) of cantilever tip relative to the chip body surface. Values are averages obtained from five experiments (116, 117, 118, 119, and 120).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211479&req=5

Figure 5: Cantilever resonance frequency and bending. (Left) Mass/stiffness ratios of each of the four cantilevers divided in three groups: clean chip, after functionalization by micro-spotting, and after the addition of BAM antibody . The values are an average of eight experiments (104, 108, 112, 116, 117, 118, 119, and 120). (Right) Average bending values (μm) of cantilever tip relative to the chip body surface. Values are averages obtained from five experiments (116, 117, 118, 119, and 120).
Mentions: The deflection values showed a clear bending of all cantilevers after the functionalization step (Figure 5, right). This was probably caused by salt deposits from the PBS buffer used in the micro-spotting of BAM-ovalbumine conjugate and ovalbumine. The cantilevers returned to their initial state after the experiment, probably caused by the removal of these deposited salts from the functionalization step. A large variation on the resonance frequency could explain the diverse signal variations obtained. The cantilevers showed a slight increase in variation of the resonance frequency after the functionalization step; but no significant difference could be seen after the experiment was performed (Figure 5, left).

Bottom Line: The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout.The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface.The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, 2800 Kongens Lyngby, Denmark. Michael.bache@nanotech.dtu.dk.

ABSTRACT
The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

No MeSH data available.


Related in: MedlinePlus