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Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue.

Bache M, Taboryski R, Schmid S, Aamand J, Jakobsen MH - Nanoscale Res Lett (2011)

Bottom Line: The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout.The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface.The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, 2800 Kongens Lyngby, Denmark. Michael.bache@nanotech.dtu.dk.

ABSTRACT
The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

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Bending signal example. (Left) An example of a bending signal from cantilever A, B, C, D normalized to zero at t = 0 under the addition of BAM antibody; (Right) The differential values of A (signal) - B(reference), C(reference) - D(signal), B(signal) - C(signal), and A(reference) - D(reference) . The antibody is added at t = 200 and enters the cantilever chamber at t = 500, gradually causing a lasting differential signal of approximately 0.02 mV for the specific antibody.
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Figure 3: Bending signal example. (Left) An example of a bending signal from cantilever A, B, C, D normalized to zero at t = 0 under the addition of BAM antibody; (Right) The differential values of A (signal) - B(reference), C(reference) - D(signal), B(signal) - C(signal), and A(reference) - D(reference) . The antibody is added at t = 200 and enters the cantilever chamber at t = 500, gradually causing a lasting differential signal of approximately 0.02 mV for the specific antibody.

Mentions: Baseline noise was typically in the range of 0.004 to 0.002 mV. (Figure 3, left). As the absolute bending signal were not suited to evaluate the experiment, the differential values of A(signal) - B(reference), C(reference) - D(signal), B(signal) - C(signal), and A(reference) - D(reference) were plotted, a signal example from chip 117 is seen in Figure 3.


Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue.

Bache M, Taboryski R, Schmid S, Aamand J, Jakobsen MH - Nanoscale Res Lett (2011)

Bending signal example. (Left) An example of a bending signal from cantilever A, B, C, D normalized to zero at t = 0 under the addition of BAM antibody; (Right) The differential values of A (signal) - B(reference), C(reference) - D(signal), B(signal) - C(signal), and A(reference) - D(reference) . The antibody is added at t = 200 and enters the cantilever chamber at t = 500, gradually causing a lasting differential signal of approximately 0.02 mV for the specific antibody.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211479&req=5

Figure 3: Bending signal example. (Left) An example of a bending signal from cantilever A, B, C, D normalized to zero at t = 0 under the addition of BAM antibody; (Right) The differential values of A (signal) - B(reference), C(reference) - D(signal), B(signal) - C(signal), and A(reference) - D(reference) . The antibody is added at t = 200 and enters the cantilever chamber at t = 500, gradually causing a lasting differential signal of approximately 0.02 mV for the specific antibody.
Mentions: Baseline noise was typically in the range of 0.004 to 0.002 mV. (Figure 3, left). As the absolute bending signal were not suited to evaluate the experiment, the differential values of A(signal) - B(reference), C(reference) - D(signal), B(signal) - C(signal), and A(reference) - D(reference) were plotted, a signal example from chip 117 is seen in Figure 3.

Bottom Line: The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout.The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface.The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, 2800 Kongens Lyngby, Denmark. Michael.bache@nanotech.dtu.dk.

ABSTRACT
The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

No MeSH data available.


Related in: MedlinePlus