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Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue.

Bache M, Taboryski R, Schmid S, Aamand J, Jakobsen MH - Nanoscale Res Lett (2011)

Bottom Line: The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout.The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface.The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, 2800 Kongens Lyngby, Denmark. Michael.bache@nanotech.dtu.dk.

ABSTRACT
The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

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Comparison of bending signals from 10 experiments. The differential signal between the two signal BAM-coated and two reference ovalbumine-coated cantilevers is shown. Plotted as signal (mV) of (B + C) - (A + D) as a function of time (s) during the addition of BAM antibody (left) and unspecific antibody (right).
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Figure 2: Comparison of bending signals from 10 experiments. The differential signal between the two signal BAM-coated and two reference ovalbumine-coated cantilevers is shown. Plotted as signal (mV) of (B + C) - (A + D) as a function of time (s) during the addition of BAM antibody (left) and unspecific antibody (right).

Mentions: Twenty chips were selected for the BAM assay based on signal stability while running in air mode and a buffer flow. Of 20 experiments, only ten gave a signal when adding BAM antibody (with five experiments giving a differential signal above 0.01 mV) (Figure 2). Seven experiments gave no differential signal, and three chips were discarded after functionalization, due to too high initial voltage difference between the cantilevers. A signal from the addition of specific BAM antibody, as well as from the addition of unspecific antibody appeared on all 10 successful experiments. The differential signals show a very diverse and distinct signal profile in between experiments, but has a similar signal profile between the specific and the unspecific antibody on each experiment (Figure 2).


Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue.

Bache M, Taboryski R, Schmid S, Aamand J, Jakobsen MH - Nanoscale Res Lett (2011)

Comparison of bending signals from 10 experiments. The differential signal between the two signal BAM-coated and two reference ovalbumine-coated cantilevers is shown. Plotted as signal (mV) of (B + C) - (A + D) as a function of time (s) during the addition of BAM antibody (left) and unspecific antibody (right).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211479&req=5

Figure 2: Comparison of bending signals from 10 experiments. The differential signal between the two signal BAM-coated and two reference ovalbumine-coated cantilevers is shown. Plotted as signal (mV) of (B + C) - (A + D) as a function of time (s) during the addition of BAM antibody (left) and unspecific antibody (right).
Mentions: Twenty chips were selected for the BAM assay based on signal stability while running in air mode and a buffer flow. Of 20 experiments, only ten gave a signal when adding BAM antibody (with five experiments giving a differential signal above 0.01 mV) (Figure 2). Seven experiments gave no differential signal, and three chips were discarded after functionalization, due to too high initial voltage difference between the cantilevers. A signal from the addition of specific BAM antibody, as well as from the addition of unspecific antibody appeared on all 10 successful experiments. The differential signals show a very diverse and distinct signal profile in between experiments, but has a similar signal profile between the specific and the unspecific antibody on each experiment (Figure 2).

Bottom Line: The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout.The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface.The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, 2800 Kongens Lyngby, Denmark. Michael.bache@nanotech.dtu.dk.

ABSTRACT
The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.

No MeSH data available.


Related in: MedlinePlus