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Study on the visible-light-induced photokilling effect of nitrogen-doped TiO2 nanoparticles on cancer cells.

Li Z, Mi L, Wang PN, Chen JY - Nanoscale Res Lett (2011)

Bottom Line: However, the visible-light-induced photokilling effects on cells were observed.The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles.The reactive oxygen species was found to play an important role on the photokilling effect for cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Fudan University, Shanghai 200433, China. lanmi@fudan.edu.cn.

ABSTRACT
Nitrogen-doped TiO2 (N-TiO2) nanoparticles were prepared by calcining the anatase TiO2 nanoparticles under ammonia atmosphere. The N-TiO2 showed higher absorbance in the visible region than the pure TiO2. The cytotoxicity and visible-light-induced phototoxicity of the pure- and N-TiO2 were examined for three types of cancer cell lines. No significant cytotoxicity was detected. However, the visible-light-induced photokilling effects on cells were observed. The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles. The cancer cells incubated with N-TiO2 were killed more effectively than that with the pure TiO2. The reactive oxygen species was found to play an important role on the photokilling effect for cells. Furthermore, the intracellular distributions of N-TiO2 nanoparticles were examined by laser scanning confocal microscopy. The co-localization of N-TiO2 nanoparticles with nuclei or Golgi complexes was observed. The aberrant nuclear morphologies such as micronuclei were detected after the N-TiO2-treated cells were irradiated by the visible light.

No MeSH data available.


Related in: MedlinePlus

Micrographs of the distributions of Golgi complexes and TiO2 nanoparticles in HeLa cells. (a) The distribution of Golgi complexes (green), (b) the distribution of TiO2 nanoparticles (red), (c) differential interference contrast (DIC) micrograph, and (d) the merged image of (a), (b), and (c), in which the yellow color denotes the co-localization of TiO2 nanoparticles with Golgi bodies. The images displayed at the bottom and right side of (d) were the X-Z and Y-Z profiles measured along the lines marked in the main image, showing the 3D distributions of TiO2 and Golgi bodies.
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Figure 7: Micrographs of the distributions of Golgi complexes and TiO2 nanoparticles in HeLa cells. (a) The distribution of Golgi complexes (green), (b) the distribution of TiO2 nanoparticles (red), (c) differential interference contrast (DIC) micrograph, and (d) the merged image of (a), (b), and (c), in which the yellow color denotes the co-localization of TiO2 nanoparticles with Golgi bodies. The images displayed at the bottom and right side of (d) were the X-Z and Y-Z profiles measured along the lines marked in the main image, showing the 3D distributions of TiO2 and Golgi bodies.

Mentions: Figure 7 is the confocal micrographs to show the distributions of Golgi complexes (fluorescence image) and TiO2 nanoparticles (reflection image) in HeLa cells. As shown in the merged image in Figure 7d, the TiO2 particles were not only found on the cell membrane but also in the cytoplasm. Some TiO2 nanoparticles aggregated around or in Golgi complexes. The co-localizations of TiO2 with Golgi complexes (yellow color) were observed. The cell viability might be influenced by the localization of TiO2 in Golgi complexes or other cell organelles, although there is no direct evidence found in this work.


Study on the visible-light-induced photokilling effect of nitrogen-doped TiO2 nanoparticles on cancer cells.

Li Z, Mi L, Wang PN, Chen JY - Nanoscale Res Lett (2011)

Micrographs of the distributions of Golgi complexes and TiO2 nanoparticles in HeLa cells. (a) The distribution of Golgi complexes (green), (b) the distribution of TiO2 nanoparticles (red), (c) differential interference contrast (DIC) micrograph, and (d) the merged image of (a), (b), and (c), in which the yellow color denotes the co-localization of TiO2 nanoparticles with Golgi bodies. The images displayed at the bottom and right side of (d) were the X-Z and Y-Z profiles measured along the lines marked in the main image, showing the 3D distributions of TiO2 and Golgi bodies.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211446&req=5

Figure 7: Micrographs of the distributions of Golgi complexes and TiO2 nanoparticles in HeLa cells. (a) The distribution of Golgi complexes (green), (b) the distribution of TiO2 nanoparticles (red), (c) differential interference contrast (DIC) micrograph, and (d) the merged image of (a), (b), and (c), in which the yellow color denotes the co-localization of TiO2 nanoparticles with Golgi bodies. The images displayed at the bottom and right side of (d) were the X-Z and Y-Z profiles measured along the lines marked in the main image, showing the 3D distributions of TiO2 and Golgi bodies.
Mentions: Figure 7 is the confocal micrographs to show the distributions of Golgi complexes (fluorescence image) and TiO2 nanoparticles (reflection image) in HeLa cells. As shown in the merged image in Figure 7d, the TiO2 particles were not only found on the cell membrane but also in the cytoplasm. Some TiO2 nanoparticles aggregated around or in Golgi complexes. The co-localizations of TiO2 with Golgi complexes (yellow color) were observed. The cell viability might be influenced by the localization of TiO2 in Golgi complexes or other cell organelles, although there is no direct evidence found in this work.

Bottom Line: However, the visible-light-induced photokilling effects on cells were observed.The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles.The reactive oxygen species was found to play an important role on the photokilling effect for cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Fudan University, Shanghai 200433, China. lanmi@fudan.edu.cn.

ABSTRACT
Nitrogen-doped TiO2 (N-TiO2) nanoparticles were prepared by calcining the anatase TiO2 nanoparticles under ammonia atmosphere. The N-TiO2 showed higher absorbance in the visible region than the pure TiO2. The cytotoxicity and visible-light-induced phototoxicity of the pure- and N-TiO2 were examined for three types of cancer cell lines. No significant cytotoxicity was detected. However, the visible-light-induced photokilling effects on cells were observed. The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles. The cancer cells incubated with N-TiO2 were killed more effectively than that with the pure TiO2. The reactive oxygen species was found to play an important role on the photokilling effect for cells. Furthermore, the intracellular distributions of N-TiO2 nanoparticles were examined by laser scanning confocal microscopy. The co-localization of N-TiO2 nanoparticles with nuclei or Golgi complexes was observed. The aberrant nuclear morphologies such as micronuclei were detected after the N-TiO2-treated cells were irradiated by the visible light.

No MeSH data available.


Related in: MedlinePlus