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Study on the visible-light-induced photokilling effect of nitrogen-doped TiO2 nanoparticles on cancer cells.

Li Z, Mi L, Wang PN, Chen JY - Nanoscale Res Lett (2011)

Bottom Line: However, the visible-light-induced photokilling effects on cells were observed.The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles.The reactive oxygen species was found to play an important role on the photokilling effect for cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Fudan University, Shanghai 200433, China. lanmi@fudan.edu.cn.

ABSTRACT
Nitrogen-doped TiO2 (N-TiO2) nanoparticles were prepared by calcining the anatase TiO2 nanoparticles under ammonia atmosphere. The N-TiO2 showed higher absorbance in the visible region than the pure TiO2. The cytotoxicity and visible-light-induced phototoxicity of the pure- and N-TiO2 were examined for three types of cancer cell lines. No significant cytotoxicity was detected. However, the visible-light-induced photokilling effects on cells were observed. The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles. The cancer cells incubated with N-TiO2 were killed more effectively than that with the pure TiO2. The reactive oxygen species was found to play an important role on the photokilling effect for cells. Furthermore, the intracellular distributions of N-TiO2 nanoparticles were examined by laser scanning confocal microscopy. The co-localization of N-TiO2 nanoparticles with nuclei or Golgi complexes was observed. The aberrant nuclear morphologies such as micronuclei were detected after the N-TiO2-treated cells were irradiated by the visible light.

No MeSH data available.


Related in: MedlinePlus

The cytotoxicities and the photokilling effects of pure TiO2 and N-550-2 samples. With the concentration of 200 μg/mL on HeLa, QGY, and KB cells. The control groups were also shown for comparison.
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Figure 3: The cytotoxicities and the photokilling effects of pure TiO2 and N-550-2 samples. With the concentration of 200 μg/mL on HeLa, QGY, and KB cells. The control groups were also shown for comparison.

Mentions: To evaluate the cytotoxicity of pure- and N-TiO2 nanoparticles, the TiO2-treated cells were further incubated in the dark for 28 h and the cell viability assays were then conducted. As shown in Figure 2a, all the surviving fractions of the treated HeLa cells were on the average values greater than 85% (with the concentration from 50 to 200 μg/mL). As shown in Figure 3, all the surviving fractions of the treated QGY or KB cells with the pure- or N-TiO2 concentration of 200 μg/mL in the dark were greater than 85%. These results indicated that the cytotoxicities of pure- and N-TiO2 nanoparticles were quite low. The cytotoxicities of these nanoparticles were quite similar, and there was no significant influence of the concentration on the cytotoxicity. Pure TiO2 is biocompatible with primary and cancer cells [4]. Nitrogen is an essential element of many biological molecules, such as proteins and nucleic acids. So, nitrogen is not toxic if it does not exceed the normal levels. It could be understood that a small amount of nitrogen doping would not lead to more cytotoxicity than pure TiO2.


Study on the visible-light-induced photokilling effect of nitrogen-doped TiO2 nanoparticles on cancer cells.

Li Z, Mi L, Wang PN, Chen JY - Nanoscale Res Lett (2011)

The cytotoxicities and the photokilling effects of pure TiO2 and N-550-2 samples. With the concentration of 200 μg/mL on HeLa, QGY, and KB cells. The control groups were also shown for comparison.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211446&req=5

Figure 3: The cytotoxicities and the photokilling effects of pure TiO2 and N-550-2 samples. With the concentration of 200 μg/mL on HeLa, QGY, and KB cells. The control groups were also shown for comparison.
Mentions: To evaluate the cytotoxicity of pure- and N-TiO2 nanoparticles, the TiO2-treated cells were further incubated in the dark for 28 h and the cell viability assays were then conducted. As shown in Figure 2a, all the surviving fractions of the treated HeLa cells were on the average values greater than 85% (with the concentration from 50 to 200 μg/mL). As shown in Figure 3, all the surviving fractions of the treated QGY or KB cells with the pure- or N-TiO2 concentration of 200 μg/mL in the dark were greater than 85%. These results indicated that the cytotoxicities of pure- and N-TiO2 nanoparticles were quite low. The cytotoxicities of these nanoparticles were quite similar, and there was no significant influence of the concentration on the cytotoxicity. Pure TiO2 is biocompatible with primary and cancer cells [4]. Nitrogen is an essential element of many biological molecules, such as proteins and nucleic acids. So, nitrogen is not toxic if it does not exceed the normal levels. It could be understood that a small amount of nitrogen doping would not lead to more cytotoxicity than pure TiO2.

Bottom Line: However, the visible-light-induced photokilling effects on cells were observed.The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles.The reactive oxygen species was found to play an important role on the photokilling effect for cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Fudan University, Shanghai 200433, China. lanmi@fudan.edu.cn.

ABSTRACT
Nitrogen-doped TiO2 (N-TiO2) nanoparticles were prepared by calcining the anatase TiO2 nanoparticles under ammonia atmosphere. The N-TiO2 showed higher absorbance in the visible region than the pure TiO2. The cytotoxicity and visible-light-induced phototoxicity of the pure- and N-TiO2 were examined for three types of cancer cell lines. No significant cytotoxicity was detected. However, the visible-light-induced photokilling effects on cells were observed. The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles. The cancer cells incubated with N-TiO2 were killed more effectively than that with the pure TiO2. The reactive oxygen species was found to play an important role on the photokilling effect for cells. Furthermore, the intracellular distributions of N-TiO2 nanoparticles were examined by laser scanning confocal microscopy. The co-localization of N-TiO2 nanoparticles with nuclei or Golgi complexes was observed. The aberrant nuclear morphologies such as micronuclei were detected after the N-TiO2-treated cells were irradiated by the visible light.

No MeSH data available.


Related in: MedlinePlus