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Porous silicon nanoparticles for cancer photothermotherapy.

Hong C, Lee J, Zheng H, Hong SS, Lee C - Nanoscale Res Lett (2011)

Bottom Line: Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis.Tumors have not recurred at all in the PSi/NIR treatment groups thereafter.Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Materials Science and Engineering, Inha University, 253 Yonghyeon-dong, Incheon, 402-751, Republic of Korea. cmlee@inha.ac.kr.

ABSTRACT
The in vitro cell tests and in vivo animal tests were performed to investigate the feasibility of the photothermal therapy based on porous silicon (PSi) in combination with near-infrared (NIR) laser. According to the Annexin V- fluorescein isothiocyanate Apoptosis assay test results, the untreated cells and the cells exposed to NIR laser without PSi treatment had a cell viability of 95.6 and 91.3%, respectively. Likewise, the cells treated with PSi but not with NIR irradiation also had a cell viability of 74.4%. Combination of these two techniques, however, showed a cell viability of 6.7%. Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis. The in vivo animal test results showed that the Murine colon carcinoma (CT-26) tumors were completely resorbed without nearly giving damage to surrounding healthy tissue within 5 days of PSi and NIR laser treatment. Tumors have not recurred at all in the PSi/NIR treatment groups thereafter. Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

No MeSH data available.


Related in: MedlinePlus

Change of body weight. Change in the body weight of the mice injected with a PSi/EtOH:PEG solution followed by NIR laser treatments (4 times for 2 min at 1.5 W/cm2 each time with a time interval of 2 min). There is no significant body weight loss for apparent side effects.
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Figure 7: Change of body weight. Change in the body weight of the mice injected with a PSi/EtOH:PEG solution followed by NIR laser treatments (4 times for 2 min at 1.5 W/cm2 each time with a time interval of 2 min). There is no significant body weight loss for apparent side effects.

Mentions: One of the important issues in PTT using inorganic nanomaterials is the toxicity of the nanomaterials to the organs of human body. We investigated the toxicity of PSi on the organs of mouse bodies by an indirect method of measuring the change in weight after laser treatment. It is widely accepted that the animals given toxic treatment lose weight. The body weight of the mouse treated with PSi followed by laser treatment increased slightly in a pattern similar to the normal mouse without tumors (Figure 7), indicating that the mouse continued to mature without any significant toxic effect. Another important issue in PTT using inorganic nanomaterials is harmless elimination of the nanomaterials from the human body in a reasonable period of time. Park et al. [31] reported that the PSi nanoparticles used for drug delivery accumulated in the organs are noticeably cleared from the body within a period of 1 week and completely cleared in 4 weeks. The experiments on this issue are also ongoing in our lab.


Porous silicon nanoparticles for cancer photothermotherapy.

Hong C, Lee J, Zheng H, Hong SS, Lee C - Nanoscale Res Lett (2011)

Change of body weight. Change in the body weight of the mice injected with a PSi/EtOH:PEG solution followed by NIR laser treatments (4 times for 2 min at 1.5 W/cm2 each time with a time interval of 2 min). There is no significant body weight loss for apparent side effects.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211409&req=5

Figure 7: Change of body weight. Change in the body weight of the mice injected with a PSi/EtOH:PEG solution followed by NIR laser treatments (4 times for 2 min at 1.5 W/cm2 each time with a time interval of 2 min). There is no significant body weight loss for apparent side effects.
Mentions: One of the important issues in PTT using inorganic nanomaterials is the toxicity of the nanomaterials to the organs of human body. We investigated the toxicity of PSi on the organs of mouse bodies by an indirect method of measuring the change in weight after laser treatment. It is widely accepted that the animals given toxic treatment lose weight. The body weight of the mouse treated with PSi followed by laser treatment increased slightly in a pattern similar to the normal mouse without tumors (Figure 7), indicating that the mouse continued to mature without any significant toxic effect. Another important issue in PTT using inorganic nanomaterials is harmless elimination of the nanomaterials from the human body in a reasonable period of time. Park et al. [31] reported that the PSi nanoparticles used for drug delivery accumulated in the organs are noticeably cleared from the body within a period of 1 week and completely cleared in 4 weeks. The experiments on this issue are also ongoing in our lab.

Bottom Line: Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis.Tumors have not recurred at all in the PSi/NIR treatment groups thereafter.Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Materials Science and Engineering, Inha University, 253 Yonghyeon-dong, Incheon, 402-751, Republic of Korea. cmlee@inha.ac.kr.

ABSTRACT
The in vitro cell tests and in vivo animal tests were performed to investigate the feasibility of the photothermal therapy based on porous silicon (PSi) in combination with near-infrared (NIR) laser. According to the Annexin V- fluorescein isothiocyanate Apoptosis assay test results, the untreated cells and the cells exposed to NIR laser without PSi treatment had a cell viability of 95.6 and 91.3%, respectively. Likewise, the cells treated with PSi but not with NIR irradiation also had a cell viability of 74.4%. Combination of these two techniques, however, showed a cell viability of 6.7%. Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis. The in vivo animal test results showed that the Murine colon carcinoma (CT-26) tumors were completely resorbed without nearly giving damage to surrounding healthy tissue within 5 days of PSi and NIR laser treatment. Tumors have not recurred at all in the PSi/NIR treatment groups thereafter. Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

No MeSH data available.


Related in: MedlinePlus